FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488012

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488012
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences528905
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN41100.7770771688677551No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA16310.3083729592270824No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAGCATT16100.30440249194089675No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT15330.2898441118915495No Hit
CTACTGTACCTTGCTTTAATCCTAACTGGCAAACTCCTTCTTTTCCTGAC14730.27849991964530496No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA14600.2760420113252852No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA12990.24560176213119558No Hit
GATGTAAGCAATTTGTGGGGCCACTTACAGTAAATGAAACCAGGAGACTA12780.24163129484500995No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA12070.2282073340202872No Hit
GTGCTGGTGGACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC11320.21402709371248144No Hit
CATGTATACAAGCCAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC10690.2021156918539246No Hit
CGTTAATATGGGCCTAAAATTTAGACAACTATTGTGGTTTCACATTTCCT10590.2002249931462172No Hit
GTATACATGCATATAAAGGCTTTAAAGCAGGATATCCACATTGCGTGAAA10350.19568731624771937No Hit
TTCTTCTACTGTACCTTGCTTTAATCCTAACTGGCAAACTCCTTCTTTTC10270.1941747572815534No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTTGATCTTC10250.1937966175400119No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA9980.18869173102920184No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATACTCTGGAT9850.18623382270918218No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG9820.18566661309686996No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGAGACATTATT9760.18453219387224548No Hit
GCACCGGACCATGCAAAACCTGCACAACGCCTGCTCAAGGAACCTCTATG9530.1801835868445184No Hit
GCTTACATCTATCCACAATATCCTCCTGCAAATGAATGTCAGGAAAAGAA9020.1705410234352105No Hit
GTCCGGTGCTGGTCGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC9000.170162883693669No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT8970.16959567408135676No Hit
GTATAAGATTCCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTCTGGA8490.16052032028436108No Hit
GCTTTATTCTTCTACTGTACCTTGCTTTAATCCTAACTGGCAAACTCCTT8410.15900776131819513No Hit
AGTCTAGATTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC8390.15862962157665364No Hit
CCTCTAAGGGATACTCATCCTCAGGCCATGCATTGGAACTCCACCAAGTT8380.1584405517058829No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTAATGTTCG8130.15371380493661432No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA7810.14766356907195055No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTGTGGCTAATATGTT7740.14634007997655535No Hit
CCCTTATCTCCACCTCTAAGGGATACTCATCCTCAGGCCATGCATTGGAA7660.1448275210103894No Hit
TCCTCAGGCCATGCATTGGAACTCCACCAAGTTCCACCAAACTCTTCAAG7600.14369310178576494No Hit
AGTCTGTACAACACCTTGAGTCCGTTTATGCCTCTGTTACCAATTTTCTT7530.14236961269036974No Hit
ATCCAGAGTATGTAGTTAATCATTACTTCCAGACGAGACATTATTTACAC7520.142180542819599No Hit
GTTTTATCCCAATGCTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC7360.1391554248872671No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAGTCTCCTGGTTTCATTTAC7150.13518495760108148No Hit
ACTAAATATTTGCCCTTAGATAAAGGGATCAAACCGTATTATCCAGAGTA7040.1331051890226033No Hit
ATCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACACCCGTG6990.13215983966874958No Hit
CTCTTTGGAAGGCGGGAATCTTATACAAAAGAGAGTCCACACGTAGCGCC6960.13159263005643734No Hit
GGATAGGGGCATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACT6910.13064728070258366No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCATCATACGG6880.1300800710902714No Hit
ATTGTGGATAGATGTAAGCAATTTGTGGGGCCACTTACAGTAAATGAAAC6850.12951286147795918No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT6740.12743309289948102No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTGTGG6580.1244079749671491No Hit
ATATTTAGTAGCATTGGGATAAAACCTAGCAGGCATAATTAATTTTAGTC6430.12157192690558796No Hit
GGATAGATGTAAGCAATTTGTGGGGCCACTTACAGTAAATGAAACCAGGA6280.11873587884402681No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAGGT6230.1177905294901731No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG6130.11589983078246567No Hit
CTTTTGTATAAGATTCCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT6120.11571076091169492No Hit
CCCCTATCCTATCCACACTTCCGGAAACTGCTGTTGTTAGATCAAGATGC6030.11400913207475824No Hit
GTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATACTCTGG6010.11363099233321673No Hit
GTATGTAGTTAATCATTACTTCCAGACGAGACATTATTTACACACTCTTT5990.11325285259167525No Hit
AGGATATTGTGGATAGATGTAAGCAATTTGTGGGGCCACTTACAGTAAAT5880.11117308401319707No Hit
GCTTTAGGGCATGGACATCGACCCATATAAAGAATTTGGAGCTTCTGTGG5870.11098401414242633No Hit
GTACAACACCTTGAGTCCGTTTATGCCTCTGTTACCAATTTTCTTTTGTC5830.11022773465934337No Hit
CATCTATCCACAATATCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGT5820.11003866478857263No Hit
GTGTTGGGATGAGTTGATGAATTTAGCCACCTGGGTGGGAGGTAATTTGG5800.10966052504703112No Hit
CAGTAAATGAAACCAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTAT5780.10928238530548964No Hit
GCCTTAGAGTCTCCGGAACATTGTACACCTCATCATACGGCTCTCAGGCA5690.10758075646855295No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG5640.10663540711469924No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA5630.1064463372439285No Hit
ATCTAAGGGCAAATATTTAGTAGCATTGGGATAAAACCTAGCAGGCATAA5540.10474470840699181No Hit
TTGTACACCTCATCATACGGCTCTCAGGCAAGCTATTCTGTGTTGGGATG5520.10436656866545033No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACAAAATGCC5510.10417749879467957No Hit
CACTTACAGTAAATGAAACCAGGAGACTAAAATTAATTATGCCTGCTAGG5510.10417749879467957No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5420.1024758699577429No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCCTATCCACACTTCCGGA5400.10209773021620139No Hit
GAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAATTTAGACA5390.10190866034543065No Hit
ATCCACAATATCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTTGCC5370.10153052060388916No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGGTGACGC5360.10134145073311843No Hit
CTCTTTTGTATAAGATTCCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC5310.10039610137926469No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5300.10020703150849396No Hit
CCACAATAGTTGTCTAAATTTTAGGCCCATATTAACGTTGACATAGCTGA5300.10020703150849396No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)