FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences528905
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN41020.7755646099015892No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAGCATT14860.2809578279653246No Hit
CTACTGTACCTTGCTTTAATCCTAACTGGCAAACTCCTTCTTTTCCTGAC14620.27642015106682677No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT14140.26734479726983107No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA13800.2609164216636258No Hit
GATGTAAGCAATTTGTGGGGCCACTTACAGTAAATGAAACCAGGAGACTA13730.2595929325682306No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA13080.24730339096813228No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA12100.22877454363259944No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA11730.22177895841408193No Hit
CATGTATACAAGCCAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC11090.20967848668475433No Hit
GTATACATGCATATAAAGGCTTTAAAGCAGGATATCCACATTGCGTGAAA10910.20627522901088097No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTTGATCTTC10750.2032501110785491No Hit
GTGCTGGTGGACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC10460.19776708482619754No Hit
TTCTTCTACTGTACCTTGCTTTAATCCTAACTGGCAAACTCCTTCTTTTC10110.19114963934922152No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGAGACATTATT9920.18755731180457738No Hit
CGTTAATATGGGCCTAAAATTTAGACAACTATTGTGGTTTCACATTTCCT9860.18642289257995293No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG9700.18339777464762103No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA9430.17829288813681096No Hit
GCACCGGACCATGCAAAACCTGCACAACGCCTGCTCAAGGAACCTCTATG9430.17829288813681096No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATACTCTGGAT9170.17337707149677162No Hit
CCTCTAAGGGATACTCATCCTCAGGCCATGCATTGGAACTCCACCAAGTT8900.16827218498596158No Hit
GTATAAGATTCCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTCTGGA8850.16732683563210785No Hit
GTCCGGTGCTGGTCGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC8740.16524706705362968No Hit
CCCTTATCTCCACCTCTAAGGGATACTCATCCTCAGGCCATGCATTGGAA8700.1644907875705467No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA8580.16222194912129778No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT8540.1614656696382148No Hit
GCTTACATCTATCCACAATATCCTCCTGCAAATGAATGTCAGGAAAAGAA8200.15503729403200953No Hit
ATCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACACCCGTG8020.15163403635813616No Hit
CTCTTTGGAAGGCGGGAATCTTATACAAAAGAGAGTCCACACGTAGCGCC7810.14766356907195055No Hit
AGTCTGTACAACACCTTGAGTCCGTTTATGCCTCTGTTACCAATTTTCTT7730.1461510101057846No Hit
GGATAGGGGCATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACT7630.14426031139807716No Hit
GCTTTATTCTTCTACTGTACCTTGCTTTAATCCTAACTGGCAAACTCCTT7560.14293682230268195No Hit
AGTCTAGATTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC7460.1410461235949745No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCATCATACGG7270.1374537960503304No Hit
GTTTTATCCCAATGCTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC7100.13423960824722775No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAGTCTCCTGGTTTCATTTAC7030.13291611915183257No Hit
TCCTCAGGCCATGCATTGGAACTCCACCAAGTTCCACCAAACTCTTCAAG7000.13234890953952033No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTAATGTTCG6820.12894565186564697No Hit
ATCCAGAGTATGTAGTTAATCATTACTTCCAGACGAGACATTATTTACAC6800.12856751212410544No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT6710.12686588328716877No Hit
GTGTTGGGATGAGTTGATGAATTTAGCCACCTGGGTGGGAGGTAATTTGG6660.12592053393331507No Hit
GGATAGATGTAAGCAATTTGTGGGGCCACTTACAGTAAATGAAACCAGGA6630.12535332432100282No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTGTGGCTAATATGTT6620.1251642544502321No Hit
ATATTTAGTAGCATTGGGATAAAACCTAGCAGGCATAATTAATTTTAGTC6490.12270634613021242No Hit
ATTGTGGATAGATGTAAGCAATTTGTGGGGCCACTTACAGTAAATGAAAC6390.12081564742250497No Hit
CTTTTGTATAAGATTCCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT6390.12081564742250497No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG6350.12005936793942201No Hit
ACTAAATATTTGCCCTTAGATAAAGGGATCAAACCGTATTATCCAGAGTA6330.11968122819788053No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTGTGG6280.11873587884402681No Hit
GTACAACACCTTGAGTCCGTTTATGCCTCTGTTACCAATTTTCTTTTGTC6240.11797959936094383No Hit
GTATGTAGTTAATCATTACTTCCAGACGAGACATTATTTACACACTCTTT6180.11684518013631937No Hit
CCCCTATCCTATCCACACTTCCGGAAACTGCTGTTGTTAGATCAAGATGC6150.11627797052400715No Hit
GTTTATGCCTCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA6140.1160889006532364No Hit
GCCTTAGAGTCTCCGGAACATTGTACACCTCATCATACGGCTCTCAGGCA6030.11400913207475824No Hit
GTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATACTCTGG5920.11192936349628006No Hit
GCTTTAGGGCATGGACATCGACCCATATAAAGAATTTGGAGCTTCTGTGG5910.1117402936255093No Hit
GTGGATATCCTGCTTTAAAGCCTTTATATGCATGTATACAAGCCAAACAG5830.11022773465934337No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT5770.1090933154347189No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG5700.1077698263393237No Hit
CATCTATCCACAATATCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGT5690.10758075646855295No Hit
CTCTTTTGTATAAGATTCCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC5610.106068197502387No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT5610.106068197502387No Hit
AGGATATTGTGGATAGATGTAAGCAATTTGTGGGGCCACTTACAGTAAAT5600.10587912763161625No Hit
GTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAT5560.10512284814853329No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCCTATCCACACTTCCGGA5450.10304307957005512No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGGTTA5380.10171959047465991No Hit
GTACAGGGCCCTGACTCTGGGATCTTGAAGAGTTTGGTGGAACTTGGTGG5350.10115238086234768No Hit
CCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCTTCCTGACTGCCGATTGG5340.10096331099157695No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACAAAATGCC5330.1007742411208062No Hit
ATCTAAGGGCAAATATTTAGTAGCATTGGGATAAAACCTAGCAGGCATAA5300.10020703150849396No Hit
CAGTAAATGAAACCAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTAT5290.10001796163772321No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)