FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488014

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488014
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences466590
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN40630.8707859148288647No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG10870.2329668445530337No Hit
CATCTAAACCCTACTAAAACTAAACGTTGGGGCTACTCTCTTAACTTCAT10500.2250369703594162No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT9150.19610364559891985No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG8250.17681476242525557No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCATTGACATTGCTGAGAGTCCAA7550.16181229773462785No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG7410.15881180479650228No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT7330.15709723740328768No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT7290.1562399537066804No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG7110.15238217707194754No Hit
GTTTAGATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA6700.1435950191817227No Hit
CCCTTTACACAATGTGGTTACCCAGCATTGATGCCTTTGTATGCATGTAT6680.14316637733341905No Hit
CTCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG6630.14209477271265994No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC6560.14059452624359717No Hit
CTATAAGGGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTC6550.14038020531944534No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG6170.132236010201676No Hit
TCTCCTGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG6120.13116440558091688No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA5790.12409181508390664No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGA5740.12302021046314753No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC5590.11980539660087014No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG5560.11916243382841467No Hit
AGTTAAGAGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAGATGTATA5470.11723354551104823No Hit
CTCCAAAAGACACCAAATACTCAAGAACCGTTTCTCTTCCAAAAGTGAGA5460.11701922458689641No Hit
GTAGTAGGCTATGTCAATGTTAATATGGGCCTGAAATTCAGACAACTATT5390.11551897811783365No Hit
ACACAGAATAGCTTGCCTTAGTGCTGTATGGTGAGGTGAACAATGTTCCG5380.11530465719368183No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT5230.11208984333140445No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATG5140.11016095501403803No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC5090.10908935039327891No Hit
GTTTCGCTCCAGACCGGCTGCGAGCAAAACAAGCTGCTAGGAGTTCCGCA5060.10844638762082341No Hit
ATTTACATACTCTGTGGAAAGCTGGTATTTTATATAAGAGAGAAACTACT5030.10780342484836794No Hit
ACATATAATAGCTTGCCTTAGTGCTGTATGGTGAGGTGAACAATGTTCCG4970.10651749930345701No Hit
GGTCCTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC4930.10566021560684968No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG4920.10544589468269787No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT4880.10458861098609057No Hit
GTATACATGCATACAAAGGCATCAATGCTGGGTAACCACATTGTGTAAAG4880.10458861098609057No Hit
GACCCAGGATCCAGGGAATTAGTAGTAGGCTATGTCAATGTTAATATGGG4850.10394564821363511No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC4830.10351700636533145No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCT4820.10330268544117961No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC4780.10244540174457233No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTGTTCG4710.10094515527550955No Hit
TTAACCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACACAGTCT4710.10094515527550955No Hit
CTCCTACCTCGTGTGCCGCTGGCCATTGATCCTTGTTGGGGTTGAAGTCC4680.10030219250305407No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)