FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences466590
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN40560.8692856683598019No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG10970.23511005379455197No Hit
CATCTAAACCCTACTAAAACTAAACGTTGGGGCTACTCTCTTAACTTCAT10060.21560684969673588No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCATTGACATTGCTGAGAGTCCAA8360.17917229259092565No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG8210.17595747872864828No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT8110.17381426948713002No Hit
CCCTTTACACAATGTGGTTACCCAGCATTGATGCCTTTGTATGCATGTAT7440.15945476756895777No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT7230.15495402816176942No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG6960.14916736320967017No Hit
GTTTAGATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA6720.14402366103002637No Hit
CTCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG6670.14295205640926725No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT6660.14273773548511542No Hit
CTATAAGGGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTC6640.14230909363681177No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG6510.13952292162283805No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC6090.1305214428084614No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6050.12966415911185408No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG6040.12944983818770225No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA5960.12773527079448765No Hit
CTCCAAAAGACACCAAATACTCAAGAACCGTTTCTCTTCCAAAAGTGAGA5730.1228058895389957No Hit
CTCCTACCTCGTGTGCCGCTGGCCATTGATCCTTGTTGGGGTTGAAGTCC5730.1228058895389957No Hit
AGTTAAGAGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAGATGTATA5720.12259156861484385No Hit
TCTCCTGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG5700.12216292676654021No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC5540.11873379198011101No Hit
GTAGTAGGCTATGTCAATGTTAATATGGGCCTGAAATTCAGACAACTATT5300.11359008980046721No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC5190.11123255963479714No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT5160.11058959686234167No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG5120.10973231316573437No Hit
ATTTACATACTCTGTGGAAAGCTGGTATTTTATATAAGAGAGAAACTACT5110.10951799224158255No Hit
GTATACATGCATACAAAGGCATCAATGCTGGGTAACCACATTGTGTAAAG5080.10887502946912705No Hit
GTTTCGCTCCAGACCGGCTGCGAGCAAAACAAGCTGCTAGGAGTTCCGCA4970.10651749930345701No Hit
ACATATAATAGCTTGCCTTAGTGCTGTATGGTGAGGTGAACAATGTTCCG4940.10587453653100151No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC4910.10523157375854604No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAAT4900.10501725283439423No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCT4830.10351700636533145No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG4820.10330268544117961No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTGTTCG4790.10265972266872414No Hit
GACCCAGGATCCAGGGAATTAGTAGTAGGCTATGTCAATGTTAATATGGG4750.10180243897211685No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATG4710.10094515527550955No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)