FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488017

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488017
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences512103
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATATAGGATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGTTT15980.31204660000039053No Hit
GTCTAGAGGCAAGTATTTAGTGCTGTTAGGATAAAATCTAGCAGGCATAA15030.29349564443090553No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14940.29173818548221747No Hit
GGATAATAAGGTTTAATCCCTTTGTCTAGAGGCAAGTATTTAGTGCTGTT14680.28666108185267414No Hit
GCTAATAGGTGTCAGCAATTTGTAGGCCCTCTTACTGTAAATGAAAAGAG14360.2804123389240055No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA12930.25248826896151755No Hit
GGATCATATTGTACAAAAAATCAAACACTGTTTTAGAAAACTTCCTGTCA12670.24741116533197421No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCTACAATTCTTTGACATACT12130.2368664116398459No Hit
GTTGACACAGAAAGGCCTTATAAGTTGGCGAGAAAGTAAAAGCCTGTTTC12090.23608531877376232No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12070.2356947723407205No Hit
TTCCTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACTCCTTCTTTTC11840.23120348836073992No Hit
ATATGATCCTGTGGCAATGACCCCCAACTTCCAATTACATACCCCATGAA11840.23120348836073992No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCCCTGAATTTCAT11110.21694854355471457No Hit
CTCTTACTGTAAATGAAAAGAGAAGACTGAAATTAATTATGCCTGCTAGA11010.2149958113895056No Hit
TGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACATGATCAGGATA10580.20659906307910714No Hit
TCCTCAAATTCATTTACATGAAGACATTGCTAATAGGTGTCAGCAATTTG9780.19097720575743551No Hit
ATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACATGATCAGGAT9480.18511900926180866No Hit
GACCACTTTATGCCTACAGCCTCCTAATACATAAATCATTAACCTAATCT9090.17750335381749374No Hit
GTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACATGATCAGG9040.17652698773488926No Hit
CCTATTAGCAATGTCTTCATGTAAATGAATTTGAGGAAAAGAAGGAGTTT8750.1708640644557833No Hit
CATGAAGACATTGCTAATAGGTGTCAGCAATTTGTAGGCCCTCTTACTGT8550.1669586001253654No Hit
GTATTTAGTGCTGTTAGGATAAAATCTAGCAGGCATAATTAATTTCAGTC8550.1669586001253654No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACACCCGTG8330.1626625893619057No Hit
CCTCTAGACAAAGGGATTAAACCTTATTATCCTGATCATGTAGTTAATCA8260.16129567684625945No Hit
GGCCAAGACTGTGCAACATCTTGAGTCCCTTTATACCGCTGTTACCAATT8000.15621857321671617No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA7750.1513367428036938No Hit
GTCTTGGCCCCCAGAACCACATCATCCATATAACTGAAAGCCAAACAGTG7720.1507509231541311No Hit
ACTACATGATCAGGATAATAAGGTTTAATCCCTTTGTCTAGAGGCAAGTA7600.14840764455588035No Hit
ATTTAGTGCTGTTAGGATAAAATCTAGCAGGCATAATTAATTTCAGTCTT7580.14801709812283856No Hit
CTCTTATATAGGATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGT7280.1421589016272117No Hit
GTATAAAGGGACTCAAGATGTTGCACAGTCTTGGCCCCCAGAACCACATC7180.14020616946200276No Hit
CTTTAATCCTGAATGGCAAACTCCTTCTTTTCCTCAAATTCATTTACATG7150.1396203498124401No Hit
CCATTTACACAATGTGGTTACCCTGCCTTAATGCCTTTGTATGCAAGTAT6770.13219996758464606No Hit
CTATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATA6750.13180942115160427No Hit
CCTCCACCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6480.1265370443055401No Hit
ATTCCTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACTCCTTCTTTT6470.1263417710890192No Hit
CAGTAAGAGGGCCTACAAATTGCTGACACCTATTAGCAATGTCTTCATGT6460.12614649787249832No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTCCTCTACTG6280.1226315799751222No Hit
CTCATAAGGTGGGAAATTTTACTGGGCTTTATTCCTCTACTGTCCCTATC6050.11814029599514161No Hit
GCTTTATTCCTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACTCCTT6050.11814029599514161No Hit
GTATAAGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAG5980.11677338347949534No Hit
ATCCAGCATCCAGGGATCTAGTAGTCAATTATGTTAATACTCACATGGGC5980.11677338347949534No Hit
TCCTCAGGCCATGCAGTGGAATTCCACAGCTTTCCACCAAGCTCTGCAAG5870.11462537809776548No Hit
TTCTTATACTGTTGACACAGAAAGGCCTTATAAGTTGGCGAGAAAGTAAA5860.1144301048812446No Hit
GCACTAAATACTTGCCTCTAGACAAAGGGATTAAACCTTATTATCCTGAT5770.11267264593255655No Hit
GTAGAGGAATAAAGCCCAGTAAAATTTCCCACCTTATGAGTCCAAGGAAT5650.11032936733430579No Hit
CTCCGAAAGAGACCAAATACTCAAGGACAGTTGGTGTTCCAAAAGTAAGG5630.109938820901264No Hit
TCCAAAGAGTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACA5510.10759554230301326No Hit
CTACATGATCAGGATAATAAGGTTTAATCCCTTTGTCTAGAGGCAAGTAT5480.10700972265345057No Hit
GTTTTAGAAAACTTCCTGTCAATAGGCCTATTGATTGGAAAGTATGTCAA5440.106228629787367No Hit
CAATAGGCCTATTGACAGGAAGTTTTCTAAAACAGTGTTTGATTTTTTGT5330.10408062440563715No Hit
ATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATATT5300.10349480475607446No Hit
GTCCCTATCTTTAATCCTGAATGGCAAACTCCTTCTTTTCCTCAAATTCA5280.10310425832303267No Hit
GATCAGGATAATAAGGTTTAATCCCTTTGTCTAGAGGCAAGTATTTAGTG5240.1023231654569491No Hit
CTGTATCAACAGTATAAGAACCTTTACCCCGTTGCCCGGCAACGGCCTGG5240.1023231654569491No Hit
TTATTATCCTGATCATGTAGTTAATCATTACTTTCAAACCCGACATTATT5230.10212789224042819No Hit
GGTGTTTCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTG5130.10017516007521925No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)