FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences506583
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24130.4763286569032123No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG19200.37900995493334755No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG14760.2913639028550109No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12360.24398765848834247No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC10080.19898022634000745No Hit
GGTTAGGATAAAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG9420.18595175913917364No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT9120.18002972859334007No Hit
GTAGTAAGCTATGTCAATGTGAATATGGGCCTAAAGATCAGACAACTATT8830.174305099065701No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8630.17035707870181194No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT8610.16996227666542305No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA7960.15713121048278367No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATCCCTTGGACTCATAAGGTG7660.1512091799369501No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7620.15041957586417232No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTAAATTTCAT7440.14686635753667218No Hit
ATGTGGTATTGGGGGCCAAATCTGTACAACATCTTGAATCCCTTTTTACC6770.1336404893176439No Hit
GGATATTATCGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG6730.13285088524486607No Hit
GGAATATGATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAATT6020.11883541295306002No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA5990.11824320989847666No Hit
GTATAGGCCTCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG5950.11745360582569886No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5930.11705880378930994No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTCTCGAGGGGAAGCACCCGCG5910.11666400175292103No Hit
GGAACATATTGTACAAAAAATCAAACAATGTTTTCGGAAACTTCCCATAA5880.11607179869833768No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCA5710.11271598138903201No Hit
GGACATTATCGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG5710.11271598138903201No Hit
CTATATGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATAT5700.11251858037083756No Hit
CTTATATAAAATACCCGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT5690.1123211793526431No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5640.11133417426167085No Hit
CCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTG5610.11074197120708748No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA5590.11034716917069859No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG5480.1081757579705596No Hit
GTGTAAAGGGAGCGGCAAAGCCTAGAAGACCCACAATTCTTTGACAGACT5320.10501734167944839No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT5270.10403033658847613No Hit
GCTTACTACTGCTTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC5240.10343813353389276No Hit
GTGGTGGAGTGAACCCTGGCCCGAATGATCCCACTCCTACCTGGTTTGCC5210.10284593047930943No Hit
ATTTACATACTCTGTGGAAGGCGGGTATTTTATATAAGAGAGAAACTACT5210.10284593047930943No Hit
CTCTTATATAAAATACCCGCCTTCCACAGAGTATGTAAATAATGCCTAGT5200.10264852946111497No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC5160.10185892538833714No Hit
AATCTGTACAACATCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTT5080.10027971724278155No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)