FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences511342
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28710.5614637561553716No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA21460.4196799793484595No Hit
ATATGATCCTGTGGCAATGTACCCCAACTTCCAATTACATACCCCATGAA19140.3743091707702477No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA17920.35045038350067076No Hit
GTTCATATACTGATTACGTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA16120.31524889408654094No Hit
GGATCATATTGTACAAAAAATCAAACACTGTTTTAGGAAACTTCCTGTCA14930.29197679830719947No Hit
GTGTAAAAGGAGCGGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT14920.291781234477121No Hit
CTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGTTT14600.285523191914609No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCCCTGAATTTCAT13880.27144259614895705No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA13000.2542329791020491No Hit
GTATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAG12200.23858787269576917No Hit
CCTTTTACACAATGTGGTTACCCTGCATTAATGCCTTTGTATGCATGTAT12170.23800118120553365No Hit
GGATAATAAGGTTTAATCCCTTTGTCTAGAGGCAAATATTTAGTGCTGTT11830.2313520109828647No Hit
GTACGGGACCCTGCAAAACCTGCACGACTGCTGCTCAAGGCAACTCTATG11620.2272451705512162No Hit
TCCTAAAACAGTGTTTGATTTTTTGTACAATATGATCCTGTGGCAATGTA11420.2233338939496462No Hit
GTATACATGCATACAAAGGCATTAATGCAGGGTAACCACATTGTGTAAAA11100.21707585138713426No Hit
AGTCTGTACAACACCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT10950.21414239393595674No Hit
CTCCGAAAGAGACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGG9450.18480781942418187No Hit
GGGTAAAGGTTCATATACTGATTACGTAGAAAGGCCTTGTAAGTTGGCGA9300.18187436197300436No Hit
TTCTTCTACTGTCCCTATCTTTAATCCTGAATGGAAGACTCCTTCTTTTC9270.18128767048276886No Hit
GTTTTAGGAAACTTCCTGTCAATCGGCCTATTGATTGGAAAGTATGTCAG8770.1715094789788439No Hit
ATGTGGTACTGGGGGCCAAGTCTGTACAACACCTTGAGTCCCTTTATACC8520.1666203832268814No Hit
GTATAAAGGGACTCAAGGTGTTGTACAGACTTGGCCCCCAGTACCACATC8310.16251354279523295No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGTCACCCGTG8100.15840670236358445No Hit
TTATTATCCTGATCATGTAGTTAATCATTACTTTCAAACCCGACATTATT7950.15547324491240697No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7800.15253978746122945No Hit
GTACAACACCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC7720.15097527682060147No Hit
CTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGT7610.148824074689738No Hit
GTACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA7520.1470640002190315No Hit
CTCAAGGACAGTTTCTCTTCCAAAAGTAAGGCAAGAAATATGAAACCACA7270.142174904467069No Hit
ATATAGAGCTGAGGCTGTATCTAGTAGATCCCGGACGGAAGGAAAGAAGT7210.14100152148659803No Hit
CTCTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGCCTTGGATGGC7210.14100152148659803No Hit
TCTGTGGAGTTACTCTCGTTTTTACCTTCTGACTTCTTTCCTTCCGTCCG7040.13767693637526351No Hit
CTGACATACTTTCCAATCAATAGGCCGATTGACAGGAAGTTTCCTAAAAC7020.13728580871510654No Hit
ATGTAAGACCTTGGGCAAGATCTGATGGGCGTTCACGGTGGTCTCCATGC6930.13552573424440004No Hit
GTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACATGATCAGG6730.13161445764283006No Hit
ATTTAGTGCTGTTAGGGTAAAACCTAGCAGGCATAATTAATTTTAGTCTC6670.13044107466235905No Hit
CATGTATACAAGCGAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6660.13024551083228056No Hit
GCTCTATATCGGGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCA6650.13004994700220204No Hit
GGCTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATACTCAAGGA6530.12770318104126005No Hit
GGGTAACCACATTGTGTAAAAGGAGCGGCAAAGCCCAAAAGACCCACAAT6520.12750761721118156No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT6340.12398746826976857No Hit
CTTTAATCCTGAATGGAAGACTCCTTCTTTTCCTAAAATTCATTTACATG6310.12340077677953307No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG6190.12105401081859107No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAGAGATGGGGTTATT6110.11948950017796307No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG6080.1189028086877276No Hit
GGTTCATATACTGATTACGTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA6060.11851168102757059No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT6030.11792498953733509No Hit
GTCTAGAGGCAAATATTTAGTGCTGTTAGGGTAAAACCTAGCAGGCATAA6000.1173382980470996No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG5960.1165560427267856No Hit
ATTACATACCCCATGAAATTCAGGGAATAACCCCATCTCTTTGTTTTGTT5920.11577378740647161No Hit
GTCTTACATAAGCGGACTCTTGGACTCCCAGCAATGTCAACGACCGACCT5890.1151870959162361No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCGAAACAGGCTTTTACTTT5860.1146004044260006No Hit
GTATGCATGTATACAAGCGAAACAGGCTTTTACTTTCTCGCCAACTTACA5840.11420927676584361No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTTCTGTGG5830.1140137129357651No Hit
CTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGCCTTGGATGGCTT5790.1132314576154511No Hit
TCCAAAGAGTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACA5690.11127581931466611No Hit
CTGCCGTACCGTCCGACCACGGGGCGCACCTCTCTTTACGCGGTCTCCCC5660.1106891278244306No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACAGCACTCAGGCA5630.11010243633419513No Hit
GCACTAAATATTTGCCTCTAGACAAAGGGATTAAACCTTATTATCCTGAT5560.10873348952364563No Hit
ATCTAGTAGATCCCGGACGGAAGGAAAGAAGTCAGAAGGTAAAAACGAGA5500.10756010654317462No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG5460.10677785122286063No Hit
GTATTAACATAATTGACTACTAGATCCCTGGATGCTGGATCTTGCAAATT5460.10677785122286063No Hit
GTACAATATGATCCTGTGGCAATGTACCCCAACTTCCAATTACATACCCC5460.10677785122286063No Hit
GGCCTAAAGATCAGGCAACTATTGTGGTTTCATATTTCTTGCCTTACTTT5400.10560446824238964No Hit
GGGATCTAGTAGTCAATTATGTTAATACTAACATGGGCCTAAAGATCAGG5390.10540890441231114No Hit
CTATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATA5370.10501777675215414No Hit
CTTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCGTTTTTACCTTCT5330.10423552143184013No Hit
CCTCTGCACGTTGCATGGAGACCACCGTGAACGCCCATCAGATCTTGCCC5320.10403995760176164No Hit
CTTTAGGCCCATGTTAGTATTAACATAATTGACTACTAGATCCCTGGATG5290.10345326611152614No Hit
CTATTGTGGTTTCATATTTCTTGCCTTACTTTTGGAAGAGAAACTGTCCT5280.10325770228144765No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC5270.10306213845136915No Hit
ACTAGATACAGCCTCAGCTCTATATCGGGAAGCCTTAGAGTCTCCTGAGC5250.10267101079121214No Hit
GTACGGCAGACGGAGAAGGGGACGATAGAGTCCCAAGCGGCCCCGTGAGG5200.10169319164081965No Hit
ATACTAACATGGGCCTAAAGATCAGGCAACTATTGTGGTTTCATATTTCT5190.10149762781074116No Hit
GTCCCGTACTGGTTGTTGTGGATCCTGGAATTAGAGGACAAACGGGCAAC5170.10110650015058414No Hit
ACTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGATGGAAATTGC5130.10032424483027015No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)