FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences644103
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN52010.8074795490783305No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG28030.4351788456194118No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT19690.3056964491703967No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTATTCCCTTAACTTCAT17420.2704536386261204No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG16350.2538413887219901No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG14720.22853487718579168No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC14670.22775860382578564No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA14530.22558503841776859No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG14040.21797755948970896No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT13500.20959380720164322No Hit
ATTATCAACAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAA13500.20959380720164322No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT13280.20617820441761642No Hit
GGATATTATCAACAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG13080.2030731109775921No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC12000.18630560640146065No Hit
CTCCAAAAGACACCAAATACTCCAGAACAGTTTCTCGTCCAAAAGTAAGA10800.1676750457613146No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA10760.1670540270733097No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATG10720.16643300838530484No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC10620.16488046166529266No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGA10500.16301740560127806No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG10340.16053333084925858No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTGTTGATAATATCCTCTTTC10310.16006756683325493No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC9930.15416788929720868No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC9160.14221327955311494No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA9160.14221327955311494No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT9050.14050547816110157No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAAT8880.13786614873708086No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC8860.13755563939307844No Hit
CCCTTTACTCAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT8750.13584783800106506No Hit
GAGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT8630.13398478193705043No Hit
TTCCTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC8600.13351901792104678No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA8540.13258748988903948No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCA8530.13243223521703826No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCGACTTACAAGGCC8520.13227698054503706No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGAGTAAAG8480.13165596185703218No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT8370.12994816046501878No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT8110.12591153899298715No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG7920.12296170022496403No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT7810.12125389883295064No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT7680.1192355880969348No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG7470.11597523998490925No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7420.11519896662490316No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAA7060.10960979843285934No Hit
GATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC6960.10805725171284716No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCCTCTACTG6870.10665995966483621No Hit
GTACAATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCC6860.106504704992835No Hit
GTTGATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCC6830.10603894097683134No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCT6810.10572843163282893No Hit
GGCCTACATATTGTTGACATCTGTTGATAATATCCTCTTTCAAATGAATG6770.10510741294482405No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG6750.10479690360082161No Hit
ATCTAAGGGCAAATATTTGGTACGGTTAGGATAGAACCTAGCAGGCATAA6720.10433113958481796No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6670.10355486622481186No Hit
GTTTGGAAGTAATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCC6670.10355486622481186No Hit
CTCCAGAACAGTTTCTCGTCCAAAAGTAAGACAGGAAATGTGAAACCACA6570.1020023195047997No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6550.10169181016079726No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA6510.10107079147279238No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCCTCTACTGTACCTGTC6500.10091553680079118No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)