FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488023

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488023
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences644103
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN51910.8059270023583185No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG30840.4788054084517539No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT17320.26890109190610817No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG15730.24421559905791465No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTATTCCCTTAACTTCAT15650.2429735616819049No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG15360.2384711761938696No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG14050.21813281416171015No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13480.20928329785764077No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA13260.20586769507361402No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT13100.20338362032159452No Hit
GGATATTATCAACAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG12660.196552414753541No Hit
ATTATCAACAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAA12480.19375783065751906No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT12210.1895659545134862No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC12120.18816866246547526No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC10670.16565673502529876No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA10340.16053333084925858No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTGTTGATAATATCCTCTTTC10200.15835976544124156No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGA10090.15665196404922815No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC10040.15587569068922208No Hit
CTCCAAAAGACACCAAATACTCCAGAACAGTTTCTCGTCCAAAAGTAAGA9840.15277059724919773No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG9680.15028652249717825No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATG9580.14873397577716607No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGAGTAAAG9250.1436105716011259No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC9090.14112649684910641No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA9000.1397292048010955No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT8710.1352268193130602No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC8680.13476105529705654No Hit
CCCTTTACTCAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT8590.13336376324904559No Hit
TTCCTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC8570.13305325390504313No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCA8540.13258748988903948No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAAT8530.13243223521703826No Hit
GAGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT8150.12653255768099203No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCGACTTACAAGGCC8100.12575628432098593No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA8080.1254457749769835No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT7980.12389322825697131No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT7970.12373797358497012No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG7860.12203017219295671No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT7540.11706202268891776No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG7370.11442269326489708No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT7290.11318065588888733No Hit
CTCCAGAACAGTTTCTCGTCCAAAAGTAAGACAGGAAATGTGAAACCACA7170.11131759982487273No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCT7130.11069658113686785No Hit
ATCTAAGGGCAAATATTTGGTACGGTTAGGATAGAACCTAGCAGGCATAA7070.10976505310486057No Hit
GGCCTACATATTGTTGACATCTGTTGATAATATCCTCTTTCAAATGAATG7030.10914403441685569No Hit
GTTTGGAAGTAATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCC6940.10774674236884473No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG6920.10743623302484229No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCCTCTACTG6890.10697046900883865No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6830.10603894097683134No Hit
GTACAATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCC6760.10495215827282282No Hit
TTATTATCCTGAACACATAGTTAATCATTACTTCCAAACTAGGCATTATT6760.10495215827282282No Hit
GTTGATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCC6650.10324435688080945No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6640.10308910220880821No Hit
GATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC6620.10277859286480578No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA6540.10153655548879605No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)