FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences389695
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAACCTTAT19900.5106557692554433No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT15720.4033923966178678No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATATTCAGG15710.40313578567854347No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT13900.35668920566083734No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA11270.289200528618535No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG10960.28124558949948036No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10700.2745737050770474No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA10490.2691848753512362No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10130.2599468815355599No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA9860.2530183861738026No Hit
CCCTTAACTTCATGGGATATATAATTGGAAGTTGGGGTACTTTACCGCAG9790.2512221095985322No Hit
GTCTATTTACAGGCAATTTTCTAAAACATTGCTTGAGTTTTTGTACAATA9240.23710850793569332No Hit
ATATAAGAGAGAGACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT9040.23197628914920643No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA9020.23146306727055774No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC8980.23043662351326039No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT8630.22145524063690836No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTTCTAAAAC8550.2194023531223136No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAAC8530.2188891312436649No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8440.21657963278974582No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA8200.21042097024596157No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8020.20580197333812342No Hit
CTACTGTACCTGTCTTTAATCCGGAGTGGCAAACTCCCTCCTTTCCTCAC7950.204005696762853No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTATATATCCCATGAA7900.20272264206623128No Hit
ATATAATTGGAAGTTGGGGTACTTTACCGCAGGAACATATTGTACAAAAA7890.20246603112690692No Hit
GGTCTATTTACAGGCAATTTTCTAAAACATTGCTTGAGTTTTTGTACAAT7870.20195280924825823No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGCCCCACAATTCTTTGACATACT7750.19887347797636615No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT7690.19733381234042008No Hit
CTATATAAGAGAGAGACTACACGCAGCGCCTCATTTTGTGGGTCACCATA7340.18835242946406805No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7050.1809107122236621No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT6970.17885782470906736No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG6870.1762917153158239No Hit
CAATAGGTCTATTTACAGGCAATTTTCTAAAACATTGCTTGAGTTTTTGT6870.1762917153158239No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6660.1709028855900127No Hit
GGATAATAAGGTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTAAGGTT6290.161408280835012No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA6280.16115166989568763No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA6220.1596120042597416No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6200.1590987823810929No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6120.15704589486649817No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC5990.15370995265528167No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAACACCGCCGTG5990.15370995265528167No Hit
GGAACATATTGTACAAAAACTCAAGCAATGTTTTAGAAAATTGCCTGTAA5970.153196730776633No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA5890.15114384326203825No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5860.15037401044406523No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC5720.14678145729352443No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5610.14395873696095665No Hit
GCATATTCAGGATAATAAGGTTTAATGCCTTTGTCTAAGGGCAAATATTT5550.14241907132501058No Hit
ACATAGAGGTGCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG5450.13985296193176716No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC5410.13882651817446978No Hit
GGATATATAATTGGAAGTTGGGGTACTTTACCGCAGGAACATATTGTACA5400.13856990723514542No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA5360.13754346347784807No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA5350.1372868525385237No Hit
TTCTAAAACATTGCTTGAGTTTTTGTACAATATGTTCCTGCGGTAAAGTA5300.136003797841902No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5290.13574718690257764No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC5250.13472074314528026No Hit
TATATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAG5220.13395091032730727No Hit
GTCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAA5210.1336942993879829No Hit
CCCTTAGACAAAGGCATTAAACCTTATTATCCTGAATATGCAGTTAATCA5040.12933191341946906No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT4960.1272790259048743No Hit
ATTTACAGGCAATTTTCTAAAACATTGCTTGAGTTTTTGTACAATATGTT4900.12573936026892826No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4840.1241996946329822No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGCACCTCTATG4800.12317325087568481No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATATTCA4800.12317325087568481No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4770.12240341805771181No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG4730.12137697430041443No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACACTGTTCACCTCACCATACAG4680.1200939196037927No Hit
CTTTCCAATCAATAGGTCTATTTACAGGCAATTTTCTAAAACATTGCTTG4610.11829764302852232No Hit
CAATTATATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTA4530.11624475551392756No Hit
CTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATA4530.11624475551392756No Hit
ACAAAAATCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCGTTAAA4490.11521831175663019No Hit
TCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT4480.11496170081730585No Hit
GATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGT4480.11496170081730585No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA4480.11496170081730585No Hit
GCTTAGATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACAT4460.11444847893865716No Hit
ATATATAATTGGAAGTTGGGGTACTTTACCGCAGGAACATATTGTACAAA4430.11367864612068412No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4390.11265220236338674No Hit
GACTTCTTTCCTTCTATTCGGGATCTCCTCGACACCGCCTCTGCTCTGTA4370.11213898048473805No Hit
GTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTATATATCCC4370.11213898048473805No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG4360.11188236954541371No Hit
GTGTAGTCTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAA4340.11136914766676505No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC4250.10905964921284594No Hit
GTTTTGAAGTAATGATTAACTGCATATTCAGGATAATAAGGTTTAATGCC4230.10854642733419725No Hit
TTCTTCTACTGTACCTGTCTTTAATCCGGAGTGGCAAACTCCCTCCTTTC4190.10751998357689989No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC4160.10675015075892685No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4140.10623692888027816No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC4140.10623692888027816No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC4100.10521048512298078No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAGACTACA4070.10444065230500775No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4060.1041840413656834No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCCCG4020.10315759760838605No Hit
CTTCAAAGACTGTGTGTTTAACGACTGGGAGGAGTTGGGGGAGGAGATTA4010.10290098666906171No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA4000.10264437572973736No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT3900.10007826633649393No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)