FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences389695
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATATTCAGG18320.47011124084219713No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAACCTTAT16820.43161959994354554No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT15040.38594285274381246No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT14970.38414657616854203No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA11340.29099680519380544No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11260.2889439176792107No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA10820.27765303634893956No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG9960.255584495567046No Hit
GTCTATTTACAGGCAATTTTCTAAAACATTGCTTGAGTTTTTGTACAATA9680.2483993892659644No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA9520.24429361423677493No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGCCCCACAATTCTTTGACATACT9210.23633867511772028No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC9170.2353122313604229No Hit
CCCTTAACTTCATGGGATATATAATTGGAAGTTGGGGTACTTTACCGCAG8930.22915356881663868No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8530.2188891312436649No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8510.21837590936501622No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8470.21734946560771884No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAAC8450.21683624372907015No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA8150.20913791554933986No Hit
ATATAAGAGAGAGACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT7820.20066975455163652No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTATATATCCCATGAA7760.19913008891569048No Hit
CTACTGTACCTGTCTTTAATCCGGAGTGGCAAACTCCCTCCTTTCCTCAC7640.19605075764379834No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT7630.195794146704474No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7470.19168837167528452No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTTCTAAAAC7430.19066192791798714No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT7410.19014870603933845No Hit
ATATAATTGGAAGTTGGGGTACTTTACCGCAGGAACATATTGTACAAAAA7310.187582596646095No Hit
GGTCTATTTACAGGCAATTTTCTAAAACATTGCTTGAGTTTTTGTACAAT7230.18552970913150027No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC7150.18347682161690554No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6910.1773181590731213No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG6780.17398221686190482No Hit
CTATATAAGAGAGAGACTACACGCAGCGCCTCATTTTGTGGGTCACCATA6610.16961983089339097No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6270.1608950589563633No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT6150.1578157276844712No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA6130.1573025058058225No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6110.1567892839271738No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA6070.15576284016987643No Hit
GGATAATAAGGTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTAAGGTT6020.15447978547325472No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6000.15396656359460603No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATATTCA5760.1478079010508218No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5740.14729467917217312No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC5720.14678145729352443No Hit
GTCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAA5710.14652484635420007No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5710.14652484635420007No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAACACCGCCGTG5650.14498518071825403No Hit
GGATATATAATTGGAAGTTGGGGTACTTTACCGCAGGAACATATTGTACA5560.1426756822643349No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC5490.14087940568906454No Hit
GGAACATATTGTACAAAAACTCAAGCAATGTTTTAGAAAATTGCCTGTAA5480.14062279474974018No Hit
GCATATTCAGGATAATAAGGTTTAATGCCTTTGTCTAAGGGCAAATATTT5450.13985296193176716No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC5450.13985296193176716No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA5150.13215463375203684No Hit
CAATAGGTCTATTTACAGGCAATTTTCTAAAACATTGCTTGAGTTTTTGT5150.13215463375203684No Hit
TTCTAAAACATTGCTTGAGTTTTTGTACAATATGTTCCTGCGGTAAAGTA5060.12984513529811775No Hit
GTGTAGTCTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAA4980.127792247783523No Hit
GACTTCTTTCCTTCTATTCGGGATCTCCTCGACACCGCCTCTGCTCTGTA4950.12702241496554997No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA4940.12676580402622564No Hit
ACATAGAGGTGCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG4910.12599597120825262No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG4810.12342986181500916No Hit
GGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG4800.12317325087568481No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACACTGTTCACCTCACCATACAG4740.12163358523973877No Hit
ATTTACAGGCAATTTTCTAAAACATTGCTTGAGTTTTTGTACAATATGTT4730.12137697430041443No Hit
TTCTTCTACTGTACCTGTCTTTAATCCGGAGTGGCAAACTCCCTCCTTTC4690.12035053054311705No Hit
GCTTAGATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACAT4690.12035053054311705No Hit
GGTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAA4690.12035053054311705No Hit
GTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTATATATCCC4690.12035053054311705No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGCACCTCTATG4640.11906747584649535No Hit
GATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGT4610.11829764302852232No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA4550.11675797739257625No Hit
GTTTTGAAGTAATGATTAACTGCATATTCAGGATAATAAGGTTTAATGCC4510.11573153363527887No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4490.11521831175663019No Hit
TATATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAG4480.11496170081730585No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC4430.11367864612068412No Hit
TCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT4410.11316542424203543No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4410.11316542424203543No Hit
ATATATAATTGGAAGTTGGGGTACTTTACCGCAGGAACATATTGTACAAA4400.11290881330271109No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4360.11188236954541371No Hit
CTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATA4350.11162575860608939No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4340.11136914766676505No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC4320.11085592578811636No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA4270.10957287109149463No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4240.1088030382735216No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAGACTACA4220.10828981639487291No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4210.10803320545554856No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG4140.10623692888027816No Hit
CAATTATATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTA4130.10598031794095382No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4000.10264437572973736No Hit
CCCTTAGACAAAGGCATTAAACCTTATTATCCTGAATATGCAGTTAATCA3990.10238776479041302No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT3980.10213115385108867No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC3970.10187454291176433No Hit
CTTCAAAGACTGTGTGTTTAACGACTGGGAGGAGTTGGGGGAGGAGATTA3960.10161793197243998No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)