FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences334469
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17420.520825547360144No Hit
ATATAAGAGAGAATCCACACGTAGCGCCTCATTTTGTGGGTCACCATATT10730.3208070105151748No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAACACACC10040.30017729595268916No Hit
GGCATACAGAGGCATGAGAGCAGGATAACCACATTGAGTAAAGGGGGCGG9220.2756608235740831No Hit
CCTTTATACCGCTGTTACCAATTTTCTGTTATCTGTGGGTATCCATTTAA9140.2732689726103167No Hit
CCTAATACAAAGACCTTTAACCTAATCTCCTCCCCCAGCTCCTCCCAGTC8790.2628046246438384No Hit
CTATATAAGAGAGAATCCACACGTAGCGCCTCATTTTGTGGGTCACCATA8420.25174231393641866No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGGG7920.23679324541287833No Hit
CTTATATAGAATGCCTGCTTTCCATAAAGTATGCAAATAGTGTCTGGTCT7570.2263288974464001No Hit
GAGTAAAGGGGGCGGCAAAGCCCAAAAGACCCACAATGCGTTGACAAACT7520.2248339905940461No Hit
CTCTTATATAGAATGCCTGCTTTCCATAAAGTATGCAAATAGTGTCTGGT7520.2248339905940461No Hit
GTCTTTGATGTATTCTTCGATCCAGGTGATTGACCTTCCTGAAAGTCCAA7310.21855538181415915No Hit
GTATAAAGGGACTCAAGATGCTGCACAGATTTGGCCCCCAATACCAGATC7200.2152665867389803No Hit
ATGTAAGACTGTTGGCAAGCTTCGAGGGGCGTTCACGGTGGTCTCCATGC6700.20031751821544No Hit
ATATGATCTTGTGGTAATGATCCCCAACTGCCAATAACATAACCCATGAA6600.19732770451073192No Hit
GGATAATAGGGTTTAATTCCCTTTTCCATAGGAAAATATTTGGTAACCTT6480.19373992806508228No Hit
GCACGGGACCATGCAAAACCTGCACAACTCTTGCTCAAGGAACCTCTATG6460.19314196532414066No Hit
ATACTAACATGGGCCTAAAAATTAGACAATTGCTGTGGTTTCACATTTCC6370.1904511329899034No Hit
CTATTATCCTGATAACGTGGTTAATCATTATTTTAAGACCAGACACTATT6320.18895622613754937No Hit
AGATCATATTGTACAAAAAATCAAAGATTGTTTTCGTAAACTTCCTGTAA6310.18865724476707857No Hit
GGGTAAAGGTTCATGTATTGTTGACATAGAAAGGCCTTGTAAGTTGGCGA6100.18237863598719165No Hit
GCTATATTGTGCTGGGGTGAGTTAATGACTTTGGCTTCCTGGGTGGGCAA6060.1811827105053084No Hit
GAATAGAGGATCCAGCTGATGGTCTGGAAAGAATCCCAGAGGATTGGGTA5940.17759493405965873No Hit
CCTCCTGCTTATAGACCACCAAATGCCCCTATCTTATCCACACTTCCGGA5920.17699697131871714No Hit
CCTCTATTCAGGGCAAATTCCAGCAGTCCCGACTGGGACTTCAACAAAAA5830.17430613898447989No Hit
CTCCAAAAGACACCAGATACTCAAGAACTGTTTCTCTTCCAAAAGTAAGG5760.17221326939118425No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4970.14859374112399057No Hit
CCCCTATCTTATCCACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC4910.14679985290116573No Hit
ATACCAGATCATCCATATAACTAAAAGCCAGACAGTGGGGGAAAGCCCTA4870.1456039274192825No Hit
CTGTATGCCTGTATAACTGCTAAACAGGCTTTTGTCTTTTCGCCAACTTA4670.1396243000098664No Hit
ATTCCTCTACTGTTCCTACTTTTAATCCTGACTGGTTAACTCCTTCTTTT4620.13812939315751235No Hit
CTCAAGAACTGTTTCTCTTCCAAAAGTAAGGCAGGAAATGTGAAACCACA4580.1369334676756291No Hit
GGCACAGGGTGTGCTTACAACATTGCCAGCAGATCCGCCTCCTGCTTCCA4580.1369334676756291No Hit
GTCCCGTGCTGGTGGTCGTGGATCCTGGAAGTAGAGGACAAACGGGCAAC4510.13484059808233348No Hit
GTTATACAGGCATACAGAGGCATGAGAGCAGGATAACCACATTGAGTAAA4500.13454161671186268No Hit
ATCTTATCCACACTTCCGGAAACTACTGTTGTTAGACGACGAGGCAGGTC4500.13454161671186268No Hit
GGCCTGGCCGTTGCCGAGCAACGGGGTAAAGGTTCATGTATTGTTGACAT4460.13334569122997944No Hit
CAATATAGCTTGCCTGAGAGCGGTATGGTTAGGTGTGCAATGTTCTGGTG4440.13274772848903785No Hit
TCCACAGGCCATGCAGTGGAACTCCACTCAGTTCCACCAGGCTCTGTTAG4360.1303558775252714No Hit
GCTTACAACATTGCCAGCAGATCCGCCTCCTGCTTCCACCAATCGGCGGT4360.1303558775252714No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC4320.12915995204338818No Hit
TGTTAGTATTGACATAGTTAACCACTAGGTCCCTGGCAGCAGGATCTTCC4320.12915995204338818No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGATCCACGACCACC4290.12826300793197576No Hit
GTCTTACATAAGCGGACTCTTGGACTTTCAGGAAGGTCAATCACCTGGAT4280.12796402656150496No Hit
CCCCCACACGGTGGCCTGTTGGGGTGGAGTCCACAGGCACAGGGTGTGCT4280.12796402656150496No Hit
CCCATAGGTATTTTCCTAAAGCCCAGGATGATGGGATGGGAATACAAGTG4270.12766504519103414No Hit
TTTAAATACCTCGAAAACAAAAAGATGGGGTTATACCCTAAATTTCATGG4220.1261701383386801No Hit
GTGGGGGAAAGCCCTACGCACCACTGAACAAATTGCACTAGTAAACTGAG4160.12437625011585529No Hit
ACCTATGGGAGTGGGCCTCAGCCCGTTTCTCCTGGCTCAGTTTACTAGTG4050.12108745504067642No Hit
GCCATGGGAAGGAGGTGTACTTCCTAGAGAGGACAACAGAGTTGTCTGTG4010.1198915295587932No Hit
TAAATACACAGTCTTTGATGTATTCTTCGATCCAGGTGATTGACCTTCCT3990.11929356681785158No Hit
TTCCCCCACTGTCTGGCTTTTAGTTATATGGATGATCTGGTATTGGGGGC3970.11869560407690997No Hit
AGATAGGGGCATTTGGTGGTCTATAAGCAGGAGGAGTGCGAATCCACACT3810.11391190214937708No Hit
TCCCATGGCTGCTCGGTTGTGCTGCCAACTGGATCCTACGCGGGACGTCC3800.11361292077890627No Hit
GTAGAGGAATAGAGTCCCGTAAAATTTCCCACCTTATGAGTCCAAGGAAT3790.11331393940843545No Hit
ATCTGGTATTGGGGGCCAAATCTGTGCAGCATCTTGAGTCCCTTTATACC3740.11181903255608142No Hit
GTTGTAAGCACACCCTGTGCCTGTGGACTCCACCCCAACAGGCCACCGTG3710.11092208844466901No Hit
AGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAGATACTCAAGAA3700.1106231070741982No Hit
GATTAAAATTAGTTATGCCATCCAGATTTTTTCCTAAGGTTACCAAATAT3680.1100251443332566No Hit
GGAAAATACAGAGCCCTTACCCTCGGATCTAACAGAGCCTGGTGGAACTG3670.10972616296278578No Hit
CCTTATGAGTCCAAGGAATACTAACATTGGGAAGCTGGAGATTGAGATCT3660.10942718159231497No Hit
CTGTTAGATCCGAGGGTAAGGGCTCTGTATTTTCCTGCTGGTGGCTCCAG3620.10823125611043174No Hit
ATCATATTGTACAAAAAATCAAAGATTGTTTTCGTAAACTTCCTGTAAAT3600.10763329336949015No Hit
ACCCAGGACCGTATCCTCCCACTCCTACCTTGTTTGCCATTGGCCAATTG3580.10703533062854853No Hit
CCAAAAGACACCAGATACTCAAGAACTGTTTCTCTTCCAAAAGTAAGGCA3550.10613838651713611No Hit
ATCTGTGGGTATCCATTTAAATACCTCGAAAACAAAAAGATGGGGTTATA3540.1058394051466653No Hit
GTTAACCACTAGGTCCCTGGCAGCAGGATCTTCCAAGTTATTGCCCACCC3500.10464347966478207No Hit
CCTTTACTCAATGTGGTTATCCTGCTCTCATGCCTCTGTATGCCTGTATA3500.10464347966478207No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC3490.10434449829431129No Hit
GGATAACCACATTGAGTAAAGGGGGCGGCAAAGCCCAAAAGACCCACAAT3490.10434449829431129No Hit
ACATAGAGGTTCCTTGAGCAAGAGTTGTGCAGGTTTTGCATGGTCCCGTG3470.10374653555336967No Hit
CTCCTACCTTGTTTGCCATTGGCCAATTGTCCTTGTTTTTGTTGAAGTCC3460.10344755418289886No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCAGAGACACAGAACCCTGCTCCGA3460.10344755418289886No Hit
CTTTTAATCCTGACTGGTTAACTCCTTCTTTTCCTGACATTCATTTACAT3450.10314857281242806No Hit
GTCCAAGGAATACTAACATTGGGAAGCTGGAGATTGAGATCTTCTGCGAC3420.10225162870101563No Hit
ATACAGCCCCAAGCGACCCCGAGAGGGATCGTCCGCTGGATTCAGCGCCG3410.10195264733054485No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTAGGAAAATACCTATGGGAG3390.10135468458960321No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCAGGCCTGTGCCAAGTGTTTGCTG3380.1010557032191324No Hit
GGGTTATACCCTAAATTTCATGGGTTATGTTATTGGCAGTTGGGGATCAT3360.10045774047819081No Hit
TTCTAAAGCATCCCGGTAGAGGGCTGAAGCGGTGTCGAGTAGGTCCCGAA3350.10015875910772No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)