FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488027

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488027
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences334469
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17220.5148459199507279No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGGG9800.2930017430613898No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAACACACC9240.2762587863150247No Hit
ATATAAGAGAGAATCCACACGTAGCGCCTCATTTTGTGGGTCACCATATT8820.26370156875525086No Hit
GGCATACAGAGGCATGAGAGCAGGATAACCACATTGAGTAAAGGGGGCGG8450.25263925804783105No Hit
CCTTTATACCGCTGTTACCAATTTTCTGTTATCTGTGGGTATCCATTTAA8270.24725759337935654No Hit
CTATATAAGAGAGAATCCACACGTAGCGCCTCATTTTGTGGGTCACCATA7660.22901972978063737No Hit
CTCTTATATAGAATGCCTGCTTTCCATAAAGTATGCAAATAGTGTCTGGT7620.22782380429875415No Hit
GTATAAAGGGACTCAAGATGCTGCACAGATTTGGCCCCCAATACCAGATC7390.2209472327779256No Hit
GGATAATAGGGTTTAATTCCCTTTTCCATAGGAAAATATTTGGTAACCTT7110.21257575440474302No Hit
GAGTAAAGGGGGCGGCAAAGCCCAAAAGACCCACAATGCGTTGACAAACT6880.2056991828839145No Hit
GTCTTTGATGTATTCTTCGATCCAGGTGATTGACCTTCCTGAAAGTCCAA6670.19942057410402758No Hit
CTTATATAGAATGCCTGCTTTCCATAAAGTATGCAAATAGTGTCTGGTCT6630.19822464862214437No Hit
ATATGATCTTGTGGTAATGATCCCCAACTGCCAATAACATAACCCATGAA6610.19762668588120275No Hit
CTATTATCCTGATAACGTGGTTAATCATTATTTTAAGACCAGACACTATT6550.19583279765837794No Hit
CTCCAAAAGACACCAGATACTCAAGAACTGTTTCTCTTCCAAAAGTAAGG6370.1904511329899034No Hit
CCTCCTGCTTATAGACCACCAAATGCCCCTATCTTATCCACACTTCCGGA5980.17879085954154195No Hit
ATACTAACATGGGCCTAAAAATTAGACAATTGCTGTGGTTTCACATTTCC5970.17849187817107118No Hit
AGATCATATTGTACAAAAAATCAAAGATTGTTTTCGTAAACTTCCTGTAA5960.17819289680060035No Hit
GCACGGGACCATGCAAAACCTGCACAACTCTTGCTCAAGGAACCTCTATG5800.17340919487306747No Hit
GTCTTACATAAGCGGACTCTTGGACTTTCAGGAAGGTCAATCACCTGGAT5780.17281123213212585No Hit
GAATAGAGGATCCAGCTGATGGTCTGGAAAGAATCCCAGAGGATTGGGTA5710.17071836253883022No Hit
CTGTATGCCTGTATAACTGCTAAACAGGCTTTTGTCTTTTCGCCAACTTA5680.1698214184274178No Hit
GCTATATTGTGCTGGGGTGAGTTAATGACTTTGGCTTCCTGGGTGGGCAA5580.16683160472270972No Hit
CCTCTATTCAGGGCAAATTCCAGCAGTCCCGACTGGGACTTCAACAAAAA5180.1548723499038775No Hit
GTCCCGTGCTGGTGGTCGTGGATCCTGGAAGTAGAGGACAAACGGGCAAC4910.14679985290116573No Hit
ATCTTATCCACACTTCCGGAAACTACTGTTGTTAGACGACGAGGCAGGTC4870.1456039274192825No Hit
GGGTAAAGGTTCATGTATTGTTGACATAGAAAGGCCTTGTAAGTTGGCGA4860.1453049460488117No Hit
TCCCATGGCTGCTCGGTTGTGCTGCCAACTGGATCCTACGCGGGACGTCC4830.14440800193739928No Hit
GCTTACAACATTGCCAGCAGATCCGCCTCCTGCTTCCACCAATCGGCGGT4760.14231513234410365No Hit
ATTCCTCTACTGTTCCTACTTTTAATCCTGACTGGTTAACTCCTTCTTTT4700.1405212441212788No Hit
CCCCTATCTTATCCACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC4640.13872735589845397No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCAGAGACACAGAACCCTGCTCCGA4550.13603652356421672No Hit
CAATATAGCTTGCCTGAGAGCGGTATGGTTAGGTGTGCAATGTTCTGGTG4360.1303558775252714No Hit
CTCCTACCTTGTTTGCCATTGGCCAATTGTCCTTGTTTTTGTTGAAGTCC4290.12826300793197576No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGATCCACGACCACC4280.12796402656150496No Hit
ATACCAGATCATCCATATAACTAAAAGCCAGACAGTGGGGGAAAGCCCTA4270.12766504519103414No Hit
CTCAAGAACTGTTTCTCTTCCAAAAGTAAGGCAGGAAATGTGAAACCACA4200.1255721755977385No Hit
CTCTAGGAAGTACACCTCCTTCCCATGGCTGCTCGGTTGTGCTGCCAACT4190.1252731942272677No Hit
ATGTAAGACTGTTGGCAAGCTTCGAGGGGCGTTCACGGTGGTCTCCATGC4110.12288134326350125No Hit
ACCTATGGGAGTGGGCCTCAGCCCGTTTCTCCTGGCTCAGTTTACTAGTG4080.12198439915208883No Hit
GTTTCCGTCCGAGGGTTTGGAACAGCAGCAAGAGGGAAACATAGAGGTTC4080.12198439915208883No Hit
GGCACAGGGTGTGCTTACAACATTGCCAGCAGATCCGCCTCCTGCTTCCA4040.1207884736702056No Hit
TGTTAGTATTGACATAGTTAACCACTAGGTCCCTGGCAGCAGGATCTTCC4030.1204894922997348No Hit
TTTAAATACCTCGAAAACAAAAAGATGGGGTTATACCCTAAATTTCATGG3950.11809764133596835No Hit
ATCTGGTATTGGGGGCCAAATCTGTGCAGCATCTTGAGTCCCTTTATACC3920.11720069722455594No Hit
GTATAACCCCATCTTTTTGTTTTCGAGGTATTTAAATGGATACCCACAGA3880.11600477174267272No Hit
GATTAAAATTAGTTATGCCATCCAGATTTTTTCCTAAGGTTACCAAATAT3870.11570579037220191No Hit
CCTAATACAAAGACCTTTAACCTAATCTCCTCCCCCAGCTCCTCCCAGTC3850.1151078276312603No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC3830.11450986489031868No Hit
GGGCTGTATCGCCCCCTTCTCCGTCTGCCGTTCCAGCCGACGACGGGTCG3800.11361292077890627No Hit
GGATAACCACATTGAGTAAAGGGGGCGGCAAAGCCCAAAAGACCCACAAT3790.11331393940843545No Hit
GGCCTGGCCGTTGCCGAGCAACGGGGTAAAGGTTCATGTATTGTTGACAT3770.11271597666749386No Hit
TCCACAGGCCATGCAGTGGAACTCCACTCAGTTCCACCAGGCTCTGTTAG3730.11152005118561063No Hit
ATCTGTGGGTATCCATTTAAATACCTCGAAAACAAAAAGATGGGGTTATA3660.10942718159231497No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC3650.10912820022184419No Hit
GTGGGGGAAAGCCCTACGCACCACTGAACAAATTGCACTAGTAAACTGAG3620.10823125611043174No Hit
CCTTATGAGTCCAAGGAATACTAACATTGGGAAGCTGGAGATTGAGATCT3620.10823125611043174No Hit
CCTCTATGTTTCCCTCTTGCTGCTGTTCCAAACCCTCGGACGGAAACTGC3610.10793227473996096No Hit
TTCCCCCACTGTCTGGCTTTTAGTTATATGGATGATCTGGTATTGGGGGC3600.10763329336949015No Hit
CCCATAGGTATTTTCCTAAAGCCCAGGATGATGGGATGGGAATACAAGTG3570.10673634925807773No Hit
AGATAGGGGCATTTGGTGGTCTATAAGCAGGAGGAGTGCGAATCCACACT3560.10643736788760692No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG3540.1058394051466653No Hit
CCTTTACTCAATGTGGTTATCCTGCTCTCATGCCTCTGTATGCCTGTATA3540.1058394051466653No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCAGGCCTGTGCCAAGTGTTTGCTG3540.1058394051466653No Hit
GCCATGGGAAGGAGGTGTACTTCCTAGAGAGGACAACAGAGTTGTCTGTG3540.1058394051466653No Hit
GTAGAGGAATAGAGTCCCGTAAAATTTCCCACCTTATGAGTCCAAGGAAT3510.10494246103525288No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCTTATAGACCACCAAATGCC3500.10464347966478207No Hit
CTCTTTACGCGGCCTCCCCGTCTGTTCCTTCTCATCTGCCGGACCGTGTG3490.10434449829431129No Hit
ATATTGTGCTGGGGTGAGTTAATGACTTTGGCTTCCTGGGTGGGCAATAA3490.10434449829431129No Hit
GTTATACAGGCATACAGAGGCATGAGAGCAGGATAACCACATTGAGTAAA3480.10404551692384048No Hit
CTTTTAATCCTGACTGGTTAACTCCTTCTTTTCCTGACATTCATTTACAT3480.10404551692384048No Hit
ATCATATTGTACAAAAAATCAAAGATTGTTTTCGTAAACTTCCTGTAAAT3470.10374653555336967No Hit
CCCCCACACGGTGGCCTGTTGGGGTGGAGTCCACAGGCACAGGGTGTGCT3470.10374653555336967No Hit
CTGTTAGATCCGAGGGTAAGGGCTCTGTATTTTCCTGCTGGTGGCTCCAG3460.10344755418289886No Hit
GTTGTAAGCACACCCTGTGCCTGTGGACTCCACCCCAACAGGCCACCGTG3420.10225162870101563No Hit
GTCCAAGGAATACTAACATTGGGAAGCTGGAGATTGAGATCTTCTGCGAC3400.10165366596007402No Hit
CTATTCCTCTACTGTTCCTACTTTTAATCCTGACTGGTTAACTCCTTCTT3370.10075672184866162No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)