FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458719
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAA38940.8488857012680966No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT16400.35751734722128364No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAC14920.32525358661838727No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13640.297349793664531No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG12900.2812179133630828No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAATGTTCCGGAT11450.24960814790754254No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC10840.2363102465779704No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG10640.23195027892893033No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT10630.23173228054647835No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT10270.22388433877820624No Hit
TCCAAAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAA9330.20339249082771807No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8990.19598054582434998No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT8390.18290064287722985No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT8270.18028466228780582No Hit
CCTCCAGCTTATAGACCTCCAAATGCCCCTATCTTATCAACACTTCCGGA8230.17941266875799783No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT8230.17941266875799783No Hit
CTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAA8180.1783226768457378No Hit
AGATAAGGGTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACT7760.16916674478275373No Hit
GGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAA7460.16262679330919363No Hit
ATCCAAATGTTACCAAATATTTGCCATTAGATAAGGGTATTAAACCTTAT6920.1508548806567855No Hit
CACCCTCTTTTCCTAATATACATTTACACCAAGACATTATCAAAAAATGT6820.1486748968322655No Hit
GGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATT6800.1482389000673615No Hit
ATTAATTGCAGTCTTCGTTTCTCATTTACTGTGAGTGGGCCTACAAATTG6770.14758490492000548No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCATCTTTTCCTAAT6580.14344293565341745No Hit
CTCTATGACTGTTTCTCTTCCAAAAGTGAGACAAGAAACGTGAAACCACA6360.13864697123947342No Hit
CAGTAAATGAGAAACGAAGACTGCAATTAATTATGCCTGCTAGGTTTTAT6280.13690298417985738No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA6260.1364669874149534No Hit
GTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACTTCCAAACC6220.13559499388514537No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC6100.13297901329572134No Hit
CAATAGGCCTGTTAACAGGTAGTTTTCTAAAACATTCTTTGATTTTCTGT6070.13232501814836534No Hit
CATTTGGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCA5990.13058103108874933No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG5870.1279650504993253No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5840.1273110553519693No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA5810.1266570602046133No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5750.12534906990990127No Hit
CTCATAAAGTGGGTAACTTTACGGGGCTTTATTCTTCTACTGTACCTGTC5700.12425907799764127No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5700.12425907799764127No Hit
GTTAACAGGTAGTTTTCTAAAACATTCTTTGATTTTCTGTATGATGTGAT5640.12295108770292923No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAATGTTCCGG5630.12273308932047725No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT5630.12273308932047725No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5540.12077110387840923No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACTTTCCACCAAACTCTGCAAG5460.1190271168187932No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA5420.1181551232889852No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACTAAAAGATGGGGTTACT5310.11575714108201317No Hit
GGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCATTTAC5240.11423115240484914No Hit
GTAAATGAGAAACGAAGACTGCAATTAATTATGCCTGCTAGGTTTTATCC5200.11335915887504115No Hit
GCAATTAATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGC5200.11335915887504115No Hit
ACATCATACAGAAAATCAAAGAATGTTTTAGAAAACTACCTGTTAACAGG5190.11314116049258914No Hit
GTTAGTATTCCTTGGACTCATAAAGTGGGTAACTTTACGGGGCTTTATTC5150.11226916696278114No Hit
GGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAG5070.11052517990316511No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACGTTTCTT5070.11052517990316511No Hit
TAGTATTCCTTGGACTCATAAAGTGGGTAACTTTACGGGGCTTTATTCTT5030.10965318637335712No Hit
GGCATAATTAATTGCAGTCTTCGTTTCTCATTTACTGTGAGTGGGCCTAC4930.10747320254883709No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT4850.10572921548922107No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCGGTACG4840.10551121710676906No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTGGATCTTC4830.10529321872431707No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT4820.10507522034186507No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT4800.10463922357696107No Hit
TAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGG4730.10311323489979704No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC4700.10245923975244105No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG4590.100061257545469No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4590.100061257545469No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)