FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463001
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC19730.42613298891363083No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15940.3442757143073125No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA15410.3328286547977218No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT15320.3308848145036404No Hit
CTCATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA11620.25097138019140347No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC11180.2414681609758942No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT10940.23628458685834372No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG10870.23477271107405817No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT9820.21209457430977469No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA9720.20993475176079532No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA9260.19999956803549018No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA8910.19244018911406238No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA8440.18228902313385933No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG7970.17213785715365626No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA7760.16760222980079958No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT7760.16760222980079958No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT7720.16673830078120783No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC7710.1665223185263099No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT7690.166090354016514No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT7460.16112276215386145No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT7100.15334740097753569No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG6930.14967570264427074No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC6900.14902775587957692No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6890.148811773624679No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT6890.148811773624679No Hit
CCTCCTGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6730.14535605754631198No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6640.14341221725223055No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA6610.14276427048753676No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA6550.14146837695814912No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCACGGAACCTCTATG6540.14125239470325118No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG6450.13930855440916973No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6440.13909257215427182No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC6130.13239712225243574No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAACAAAACAG6120.1321811399975378No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA6080.13131721097794605No Hit
GGCAAGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA6020.13002131744855844No Hit
CACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAACCT5990.12937337068386462No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA5980.12915738842896668No Hit
CATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT5970.12894140617406874No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5930.12807747715447698No Hit
TCCTAGTACAAAGACCTTTAACCTAACCTCCTCCCCCAACTCCTCCCACT5850.1263496191152935No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA5660.12224595627223268No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT5640.1218139917624368No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT5570.12030211597815123No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC5560.1200861337232533No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC5380.11619845313509043No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG5330.11511854186060073No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAGGTTCCCGTGGGCGTTC5180.11187880803713167No Hit
CCTTTGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT5160.11144684352733579No Hit
CTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAA5120.11058291450774405No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT5100.11015094999794817No Hit
GGATATTGTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATG5090.10993496774305023No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC5060.10928702097835641No Hit
CTACAAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAA5030.1086390742136626No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA5000.1079911274489688No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG4970.10734318068427498No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT4940.10669523391958118No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT4920.1062632694097853No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT4920.1062632694097853No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT4920.1062632694097853No Hit
GGCAGAGACAGTATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCAC4910.10604728715488734No Hit
GTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG4900.10583130489998942No Hit
CCTCTTGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4760.1028075533314183No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATATCCTCCTGC4760.1028075533314183No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAACAAAAC4740.10237558882162243No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA4730.10215960656672447No Hit
ATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC4720.10194362431182655No Hit
GTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACACACTCTTT4660.10064773078243892No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT4660.10064773078243892No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG4650.10043174852754097No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG4640.10021576627264304No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)