FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488032

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488032
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences616248
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT54940.8915241915592423No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA48760.7912398904337216No Hit
GGATAATAAGGTTTGATTCCTTTATCTAAGGGCAAATATTTAGTAACATT28980.4702652178992873No Hit
CCCTTACAGTAAATGAAAACAGAAGACTAAAATTAATTATGCCTGCTAGG28950.46977840090353234No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAATACAAAGACC26850.43570121120068545No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24730.4012994768340019No Hit
ATTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT20750.3367150887305111No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT19750.32048785553867926No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTCTGGA19420.3151328685853747No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA19160.31091378795549846No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT18550.301015175708481No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC18370.29809427373395125No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC17880.2901429294699537No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGAATCAAAC17170.278621593903753No Hit
CATTTAAACCCTCATAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT17160.2784593215718347No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA15330.2487634848307824No Hit
GCTTTAATACTAAATGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAG15160.246004855188171No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG14880.2414612298944581No Hit
CGTTAATATGGGCCTAAAAATCAGACAATTATTGTGGTTTCACATTTCCT14860.24113668523062143No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT13940.22620763069413613No Hit
CTCATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA13620.22101491607274992No Hit
ATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGAATCAAACCTTAT13570.22020355441315834No Hit
CTCCAAAAGACACCAAATATTCAAGAACCGTTTCTCTCCCAAACGTAAGA13380.21712038010671028No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT13310.21598447378328206No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT13180.2138749334683439No Hit
GTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG12720.20641040620010126No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12610.20462541054899974No Hit
AGATAAAGGAATCAAACCTTATTATCCAGAGCATGTAGTTAATCATTACT12590.2043008658851631No Hit
ATGCAGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGA12550.20365177655748984No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG12470.20235359790214327No Hit
ACTACATGCTCTGGATAATAAGGTTTGATTCCTTTATCTAAGGGCAAATA12050.1955381599615739No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATACTAAATGGCAAACTCCTT12020.19505134296581894No Hit
CGTGTGGACTCTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAA11420.18531500305071982No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG11280.18304319040386338No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTTGC11220.18206955641235348No Hit
CCTTTATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGG11190.1815827394165985No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGAAGACTA11040.17914865443782374No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC10900.17687684179096727No Hit
CAGTAAATGAAAACAGAAGACTAAAATTAATTATGCCTGCTAGGTTTTAT10840.17590320779945737No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC10820.1755786631356207No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA10780.17492957380794746No Hit
TCTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG10450.16957458685464294No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA10380.1684386805312147No Hit
GTTGTGAATAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG10300.16714050187586815No Hit
GCTCTGGATAATAAGGTTTGATTCCTTTATCTAAGGGCAAATATTTAGTA9900.16064960859913544No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA9530.15464553231815764No Hit
ACTAAATATTTGCCCTTAGATAAAGGAATCAAACCTTATTATCCAGAGCA9450.15334735366281108No Hit
ATATTTGCCCTTAGATAAAGGAATCAAACCTTATTATCCAGAGCATGTAG9300.15091326868403632No Hit
CTTTAATACTAAATGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAGG9170.14880372836909816No Hit
CCTTACAGTAAATGAAAACAGAAGACTAAAATTAATTATGCCTGCTAGGT9160.14864145603717985No Hit
CTACGGTACCTTGCTTTAATACTAAATGGCAAACTCCTTCTTTTCCTGAC9090.1475055497137516No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCGGGATCTTC9080.1473432773818333No Hit
GATTAACTACATGCTCTGGATAATAAGGTTTGATTCCTTTATCTAAGGGC9010.14620737105840506No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC8940.14507146473497684No Hit
CTTCAAAGACTGTGTGTTTAATGAGTGGGAGGAGTTGGGGGAGGAGATTA8910.14458464773922186No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC8900.14442237540730354No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA8850.14361101374771196No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTA8820.143124196751957No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG8700.1411769287689372No Hit
GTGGATATCCTGCTTTGATGCCTTTATATGCATGTATACAAGCAAAACAG8600.139554205449754No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT8520.13825602679440743No Hit
GCCTAGCAGCCATGGAAAGGATGTATATTTGCGGGAGAGCACGACAGAAT8340.13533512481987772No Hit
AATGAAAACAGAAGACTAAAATTAATTATGCCTGCTAGGTTTTATCCCAA8300.13468603549220443No Hit
GTATACATGCATATAAAGGCATCAAAGCAGGATATCCACATTGCGTGAAA8290.1345237631602861No Hit
TTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTCCTAAAAC8270.13419921849644947No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT8180.13273876750918462No Hit
CCCTTAGATAAAGGAATCAAACCTTATTATCCAGAGCATGTAGTTAATCA8170.1325764951772663No Hit
CTCTAAGGCCTCCCGATACAAAGCAGAGGCGGTGTCGAGGAGATCTCGAA8090.13127831652191976No Hit
GAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACA8020.13014241019849151No Hit
TGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGAA8010.1299801378665732No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA7860.1275460528877984No Hit
CATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT7800.1265724188962885No Hit
ACCTTGCTTTAATACTAAATGGCAAACTCCTTCTTTTCCTGACATTCATT7770.12608560190053356No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA7770.12608560190053356No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC7760.1259233295686152No Hit
ATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATTTACAC7750.1257610572366969No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTC7710.12511196790902365No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA7610.12348924458984045No Hit
ATGTGGATATCCTGCTTTGATGCCTTTATATGCATGTATACAAGCAAAAC7610.12348924458984045No Hit
ATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGATAATAAG7610.12348924458984045No Hit
CTTCTACGGTACCTTGCTTTAATACTAAATGGCAAACTCCTTCTTTTCCT7500.12170424893873895No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC7420.1204060702833924No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT7410.12024379795147408No Hit
ATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGCATAA7400.12008152561955576No Hit
CATATAAAGGCATCAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA7380.11975698095571914No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC7340.11910789162804586No Hit
TTATTCTTCTACGGTACCTTGCTTTAATACTAAATGGCAAACTCCTTCTT7280.11813425763653594No Hit
TTGTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAAC7260.1178097129726993No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT7250.11764744064078098No Hit
CTACATGCTCTGGATAATAAGGTTTGATTCCTTTATCTAAGGGCAAATAT7120.11553790032584285No Hit
ACATTGACCCGTATAAAGAATTTGGAGCCTCTGTGGAGTTACTCTCGTTT7070.11472653866625125No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG6990.11342836001090469No Hit
GGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGA6980.11326608767898638No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG6910.11213018135555815No Hit
GTTAAGGGAATATCCCCATCTTTTTGTTTTATGAGGGTTTAAATGTATAC6870.11148109202788486No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAGTCTTCTGTTTTCATTTAC6840.11099427503212993No Hit
AATTAATTATGCCTGCTAGGTTTTATCCCAATGTTACTAAATATTTGCCC6770.10985836870870168No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCACCGGAACTTGCCC6740.10937155171294674No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT6690.10856019005335514No Hit
AATTATGCCTGCTAGGTTTTATCCCAATGTTACTAAATATTTGCCCTTAG6650.10791110072568186No Hit
ACGTTAATATGGGCCTAAAAATCAGACAATTATTGTGGTTTCACATTTCC6640.10774882839376355No Hit
GACTAAAATTAATTATGCCTGCTAGGTTTTATCCCAATGTTACTAAATAT6590.10693746673417195No Hit
ATACTAAATGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAGGAGGAC6490.10531474341498877No Hit
GCCCCTTACAGTAAATGAAAACAGAAGACTAAAATTAATTATGCCTGCTA6480.10515247108307045No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6460.10482792641923382No Hit
GTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG6420.10417883709156053No Hit
GCTTACATCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAA6400.10385429242772391No Hit
CACCAATTTATGCCTACAGCCTCCTAATACAAAGACCTTTAACCTAATCT6320.10255611377237735No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA6260.10158247978086744No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC6200.10060884578935753No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA6180.1002843011255209No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)