FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences616248
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT55590.9020718931339331No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA48810.7920512520933131No Hit
GGATAATAAGGTTTGATTCCTTTATCTAAGGGCAAATATTTAGTAACATT34830.5651945320715037No Hit
CCCTTACAGTAAATGAAAACAGAAGACTAAAATTAATTATGCCTGCTAGG25060.4066544637873064No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24650.4000012981786554No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAATACAAAGACC23310.37825680570160064No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT21140.34304370967532555No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC19740.3203255832067609No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGAATCAAAC19730.32016331087484257No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT19720.3200010385429243No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTCTGGA18780.30474743934260234No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC17500.28397658085705757No Hit
ATTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT17460.28332749152938425No Hit
CATTTAAACCCTCATAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT16080.2609339097246563No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA15650.2539561994521686No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA15100.2450312211966611No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT15000.2434084978774779No Hit
GTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG14870.24129895756253975No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT14800.24016305123911152No Hit
CGTTAATATGGGCCTAAAAATCAGACAATTATTGTGGTTTCACATTTCCT14390.2335098856304605No Hit
GCTTTAATACTAAATGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAG13810.22409809037919798No Hit
CTCCAAAAGACACCAAATATTCAAGAACCGTTTCTCTCCCAAACGTAAGA13690.22215082239617814No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG13210.21436175046409886No Hit
CCTTTATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGG12600.20446313821708145No Hit
ATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGAATCAAACCTTAT12510.2030026872298166No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATACTAAATGGCAAACTCCTT12060.19570043229349224No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11520.18693772636990302No Hit
GCTCTGGATAATAAGGTTTGATTCCTTTATCTAAGGGCAAATATTTAGTA11460.18596409237839312No Hit
AGATAAAGGAATCAAACCTTATTATCCAGAGCATGTAGTTAATCATTACT11440.18563954771455649No Hit
ATGCAGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGA11430.18547727538263814No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC11110.18028456076125196No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG11070.1796354714335787No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT11070.1796354714335787No Hit
CTTCAAAGACTGTGTGTTTAATGAGTGGGAGGAGTTGGGGGAGGAGATTA10950.17768820345055888No Hit
ACTACATGCTCTGGATAATAAGGTTTGATTCCTTTATCTAAGGGCAAATA10770.17476730147602912No Hit
CGTGTGGACTCTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAA10300.16714050187586815No Hit
TCTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG10220.16584232322052161No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA10100.16389505523750178No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC10010.16243460425023692No Hit
GATTAACTACATGCTCTGGATAATAAGGTTTGATTCCTTTATCTAAGGGC9970.16178551492256366No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA9970.16178551492256366No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG9870.16016279160338046No Hit
GTTGTGAATAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG9830.1595137022757072No Hit
CTCATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA9550.15497007698199425No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTTGC9410.1526982643351378No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA9400.15253599200321946No Hit
ATATTTGCCCTTAGATAAAGGAATCAAACCTTATTATCCAGAGCATGTAG9290.15075099635211797No Hit
GTATACATGCATATAAAGGCATCAAAGCAGGATATCCACATTGCGTGAAA9070.14718100504991496No Hit
CAGTAAATGAAAACAGAAGACTAAAATTAATTATGCCTGCTAGGTTTTAT8950.14523373706689513No Hit
ATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGCATAA8740.14182601809661044No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGAAGACTA8660.1405278394412639No Hit
GAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACA8590.13939193311783568No Hit
GTGGATATCCTGCTTTGATGCCTTTATATGCATGTATACAAGCAAAACAG8510.13809375446248912No Hit
ACTAAATATTTGCCCTTAGATAAAGGAATCAAACCTTATTATCCAGAGCA8460.13728239280289753No Hit
ACATTGACCCGTATAAAGAATTTGGAGCCTCTGTGGAGTTACTCTCGTTT8440.1369578481390609No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCGGGATCTTC8400.13630875881138763No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC8390.1361464864794693No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA8270.13419921849644947No Hit
ATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGATAATAAG8240.13371240150069452No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA8180.13273876750918462No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAGTCTTCTGTTTTCATTTAC8080.13111604419000142No Hit
GCCTAGCAGCCATGGAAAGGATGTATATTTGCGGGAGAGCACGACAGAAT8020.13014241019849151No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTA8000.12981786553465488No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC7930.12868195921122666No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG7890.1280328698835534No Hit
CCCTTAGATAAAGGAATCAAACCTTATTATCCAGAGCATGTAGTTAATCA7880.12787059755163507No Hit
CTTTAATACTAAATGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAGG7820.12689696356012514No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA7810.12673469122820682No Hit
ATGTGGATATCCTGCTTTGATGCCTTTATATGCATGTATACAAGCAAAAC7730.1254365125728603No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT7670.12446287858135037No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA7570.12284015526216718No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC7510.12186652127065727No Hit
CCTTACAGTAAATGAAAACAGAAGACTAAAATTAATTATGCCTGCTAGGT7480.1213797042749023No Hit
ATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATTTACAC7460.12105515961106568No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC7360.11943243629188248No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCACCGGAACTTGCCC7300.11845880230037258No Hit
CTACGGTACCTTGCTTTAATACTAAATGGCAAACTCCTTCTTTTCCTGAC7170.11634926198543444No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT7170.11634926198543444No Hit
GTATAAAGAATTTGGAGCCTCTGTGGAGTTACTCTCGTTTTTGCCTTCTG7160.11618698965351612No Hit
CTACATGCTCTGGATAATAAGGTTTGATTCCTTTATCTAAGGGCAAATAT7010.11375290467474135No Hit
AATGAAAACAGAAGACTAAAATTAATTATGCCTGCTAGGTTTTATCCCAA6990.11342836001090469No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6880.11164336435980318No Hit
TGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGAA6860.11131881969596656No Hit
GACTAAAATTAATTATGCCTGCTAGGTTTTATCCCAATGTTACTAAATAT6860.11131881969596656No Hit
AATTAATTATGCCTGCTAGGTTTTATCCCAATGTTACTAAATATTTGCCC6750.10953382404486506No Hit
GGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGA6710.10888473471719178No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6680.10839791772143682No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCCTCTGTGG6650.10791110072568186No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTC6640.10774882839376355No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA6620.10742428372992692No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT6590.10693746673417195No Hit
TTATTCTTCTACGGTACCTTGCTTTAATACTAAATGGCAAACTCCTTCTT6570.10661292207033532No Hit
TTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTCCTAAAAC6530.10596383274266205No Hit
TTGTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAAC6510.10563928807882542No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT6500.1054770157469071No Hit
GTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG6460.10482792641923382No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA6440.10450338175539717No Hit
GTCTGGAAGTAATGATTAACTACATGCTCTGGATAATAAGGTTTGATTCC6400.10385429242772391No Hit
ACCTTGCTTTAATACTAAATGGCAAACTCCTTCTTTTCCTGACATTCATT6390.1036920200958056No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT6360.10320520310005063No Hit
GTTAAGGGAATATCCCCATCTTTTTGTTTTATGAGGGTTTAAATGTATAC6320.10255611377237735No Hit
CTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTG6300.10223156910854073No Hit
CTTCTACGGTACCTTGCTTTAATACTAAATGGCAAACTCCTTCTTTTCCT6280.10190702444470409No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6230.10109566278511249No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)