FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences472041
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG21570.4569518325738654No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA17470.37009497056399765No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC15610.33069161365220395No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT15430.32687838556396587No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15370.32560730953455314No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT15100.319887467402196No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT13930.29510148482864834No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT12430.26332458409333087No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG11430.2421399836031192No Hit
CATCAATAGATGTCAACAATATGTAGGCCCCCTTACAGTTAACGAAAAAA11400.24150444558841286No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT11260.23853860151978323No Hit
TCCTCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG11040.23387798941193666No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA9950.21078677487760597No Hit
ATATGTTCCTGTGGTAAGGAACCCCAACTTCCAATTACATATCCCATGAA9890.20951569884819327No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAACTCAGACAAATATT9390.19892339860308744No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT9310.1972286305638705No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTTCCTTACCACAG9080.19235617245112183No Hit
ATGTAAGACCTTGGGCAAGATCTGGCGGGCGTTCACGGTGGTCTCCATGC9000.1906614044119049No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT8980.19023771240210066No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC8940.1893903283824922No Hit
CCCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG8790.18621263830896045No Hit
CTATTGATGATATCCTCTTTCAAATGAATGTTAGGAAAAGAGGGAGTTTG8710.18451787026974353No Hit
GGATATCATCAATAGATGTCAACAATATGTAGGCCCCCTTACAGTTAACG8350.1768914140932673No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT8280.1754084920589525No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGATTGGCAAACTCCCTCTTTTC8220.1741374160295398No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAAT8160.1728663400001271No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8030.1701123419363996No Hit
CTACTGTACCTGTCTTTAATCCTGATTGGCAAACTCCCTCTTTTCCTAAC8000.16947680392169323No Hit
CTTTAATCCTGATTGGCAAACTCCCTCTTTTCCTAACATTCATTTGAAAG7510.15909634968148953No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC7330.15528312159325142No Hit
ACAATATGTAGGCCCCCTTACAGTTAACGAAAAAAGGAGATTAAAATTGA7290.15443573757364298No Hit
TCCGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7240.15337650754913237No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC7110.15062250948540487No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG7040.14913958745109004No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC7020.1487158954412858No Hit
GCTTACTACTGCTTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC6950.147232973406971No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTGTCTTCCAAAAGTAAGA6900.14617374338246042No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG6850.14511451335794984No Hit
GGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG6730.14257236129912443No Hit
GTACAAAGATCATTAATCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTA6700.1419368232844181No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6620.14024205524520117No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAAATGTATA6570.13918282522069056No Hit
CTGTAAGGGGGCCTACATATTGTTGACATCTATTGATGATATCCTCTTTC6470.1370643651716694No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC6450.13664067316186518No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCAAG6440.13642882715696306No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG6410.13579328914225672No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG6280.1330392910785292No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6220.1317682150491165No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6130.12986160100499747No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACGAGAAAAACCCC6100.1292260629902911No Hit
ACATATTGTTGACATCTATTGATGATATCCTCTTTCAAATGAATGTTAGG6070.12859052497558474No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6050.12816683296578052No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATCATT6020.12753129495107418No Hit
ATTTATGCCTACAGCCTCCCAGTACAAAGATCATTAATCTAGTCTCCTCC6010.12731944894617206No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5960.12626021892166148No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCT5950.12604837291675935No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5920.125412834902053No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5750.12181145281871701No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT5720.12117591480401066No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA5670.12011668477950009No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC5640.11948114676479374No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC5610.11884560875008737No Hit
GTACAATATGTTCCTGTGGTAAGGAACCCCAACTTCCAATTACATATCCC5610.11884560875008737No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCA5570.11799822473047893No Hit
ATCTGACCCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTG5520.11693899470596833No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC5510.11672714870106622No Hit
GCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACA5500.1165153026961641No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTGGGGGAGGAGACTA5470.11587976468145776No Hit
TTGTTCACCTCACCATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTG5390.11418499664224081No Hit
GTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG5370.1137613046324366No Hit
CAATAGGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGT5310.11249022860302389No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGG5250.1112191525736112No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT5250.1112191525736112No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA5250.1112191525736112No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5240.11100730656870908No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCACGAA5220.11058361455890484No Hit
CCTTCTCGGCACCGACAACTCTGTTGTCCTCTCTCGGAAATACACCTCCT5210.11037176855400273No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5100.10804146250007944No Hit
ATGTAGGCCCCCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATG5090.10782961649517732No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT5080.10761777049027521No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGGCCGACCACG5050.10698223247556884No Hit
CCGATAAGGTTTGCTCCAGACCGGCTGCGAGCAAAACAAGCTGCTAGGAG5040.10677038647066675No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5010.1061348484559604No Hit
CCCAAGTGTCCTGGCCAAAATTTGCAGTCCCCAACCTCCAATCACTCACC4950.10486377242654768No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT4930.10444008041674346No Hit
GGAACATATTGTACACAAAATCAAACAATGTTTTCGGAAACTTCCTATAA4900.10380454240203711No Hit
ACTAAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACAAATGG4900.10380454240203711No Hit
AGGTAGGAGTGGGATCTTTCGGGCCAGGGTTCACTCCACCACACGGCAAT4890.10359269639713499No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGATCTGGCGGGCGTTC4870.10316900438733076No Hit
GACCAATTTATGCCTACAGCCTCCCAGTACAAAGATCATTAATCTAGTCT4820.10210977436282019No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT4760.10083869833340747No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)