FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences472041
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG23050.4883050412993787No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC15430.32687838556396587No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15310.3243362335051404No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT15030.3184045453678812No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA14820.31395577926493673No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT14180.3003976349512013No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT13310.28196703252471716No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG12230.25908766399528854No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT11440.24235182960802132No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT11330.24002152355409806No Hit
CATCAATAGATGTCAACAATATGTAGGCCCCCTTACAGTTAACGAAAAAA10580.2241330731864393No Hit
TCCTCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG10250.21714215502466946No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA10150.2150236949756483No Hit
ATATGTTCCTGTGGTAAGGAACCCCAACTTCCAATTACATATCCCATGAA10140.21481184897074618No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT9850.20866831482858483No Hit
CTATTGATGATATCCTCTTTCAAATGAATGTTAGGAAAAGAGGGAGTTTG9390.19892339860308744No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAACTCAGACAAATATT9310.1972286305638705No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTTCCTTACCACAG8880.1881192523530795No Hit
CCCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG8870.18790740634817737No Hit
GGATATCATCAATAGATGTCAACAATATGTAGGCCCCCTTACAGTTAACG8580.181763872206016No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGATTGGCAAACTCCCTCTTTTC8380.17752695210797367No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC8200.17371372401973556No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT8200.17371372401973556No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT8110.17180710997561652No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTGTCTTCCAAAAGTAAGA8030.1701123419363996No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA7860.16651095985306363No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAAT7810.16545172982855302No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7610.1612148097305107No Hit
CTACTGTACCTGTCTTTAATCCTGATTGGCAAACTCCCTCTTTTCCTAAC7570.16036742571090223No Hit
CTTTAATCCTGATTGGCAAACTCCCTCTTTTCCTAACATTCATTTGAAAG7280.15422389156874086No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG7130.1510462014952091No Hit
GGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG6990.1480803574265795No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6970.14765666541677525No Hit
ACAATATGTAGGCCCCCTTACAGTTAACGAAAAAAGGAGATTAAAATTGA6970.14765666541677525No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCAAG6950.147232973406971No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC6870.14553820536775408No Hit
CCTTCTCGGCACCGACAACTCTGTTGTCCTCTCTCGGAAATACACCTCCT6860.14532635936285196No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6800.14405528333343925No Hit
GCTTACTACTGCTTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC6720.14236051529422233No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG6640.14066574725500539No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC6630.14045390125010326No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGGCCGACCACG6580.13939467122559268No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC6500.13769990318637576No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAAATGTATA6440.13642882715696306No Hit
ATGTAAGACCTTGGGCAAGATCTGGCGGGCGTTCACGGTGGTCTCCATGC6420.13600513514715884No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCA6330.1340985211030398No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6160.13049713901970378No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6150.13028529301480168No Hit
GTACAAAGATCATTAATCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTA6080.12880237098048686No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA6050.12816683296578052No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCT6010.12731944894617206No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTGGGGGAGGAGACTA6010.12731944894617206No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5990.12689575693636782No Hit
CTGTAAGGGGGCCTACATATTGTTGACATCTATTGATGATATCCTCTTTC5970.12647206492656357No Hit
ATTTATGCCTACAGCCTCCCAGTACAAAGATCATTAATCTAGTCTCCTCC5920.125412834902053No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC5900.12498914289224877No Hit
ACATATTGTTGACATCTATTGATGATATCCTCTTTCAAATGAATGTTAGG5850.12392991286773818No Hit
TCCGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5840.12371806686283607No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC5810.1230825288481297No Hit
ATCTGACCCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTG5780.12244699083342338No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACGAGAAAAACCCC5780.12244699083342338No Hit
GTACAATATGTTCCTGTGGTAAGGAACCCCAACTTCCAATTACATATCCC5740.1215996068138149No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG5610.11884560875008737No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA5590.11842191674028317No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC5490.11630345669126198No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC5470.11587976468145776No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG5430.11503238066184929No Hit
TTGTTCACCTCACCATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTG5390.11418499664224081No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC5370.1137613046324366No Hit
CAATAGGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGT5360.11354945862753447No Hit
GCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACA5330.11291392061282812No Hit
GGAACATATTGTACACAAAATCAAACAATGTTTTCGGAAACTTCCTATAA5310.11249022860302389No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT5300.11227838259812177No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5220.11058361455890484No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCACGAA5180.10973623053929638No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT5170.10952438453439425No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATCATT5150.10910069252459004No Hit
GTTTGGAAGTAATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCC5120.10846515450988369No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5100.10804146250007944No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5060.10719407848047097No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT4970.10528746443635192No Hit
ATCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAA4960.1050756184314498No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4940.10465192642164557No Hit
GATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC4930.10444008041674346No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4870.10316900438733076No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC4870.10316900438733076No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC4860.10295715838242864No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4850.10274531237752652No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4850.10274531237752652No Hit
GTATGCATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA4770.1010505443383096No Hit
ATGTTAATATGGGCCTAAAACTCAGACAAATATTGTGGTTTCACATTTCC4750.10062685232850535No Hit
CCCAAGTGTCCTGGCCAAAATTTGCAGTCCCCAACCTCCAATCACTCACC4730.10020316031870112No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)