FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488040

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488040
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences594434
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN39170.6589461571848179No Hit
GTATGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCAGG22330.3756514600443447No Hit
ATCCTAACCTTACCAAATATTTACCCTTAGATAAAGGCATTAAACCTTAT18580.3125662394815909No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA17310.2912013781176716No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCA17310.2912013781176716No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC16580.27892078851478885No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG16140.2715187893020924No Hit
GTGTAAAAGGGGCGGCAAAGCCCAGAAGACCCACAATTCTTTGACATACT14990.25217265499618124No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAATATGTT13810.23232183892576805No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG13670.2299666573580919No Hit
CTGTAAGAGGGCCTACAAATTGTTGACATCTGTTGATAATATCCTCTTTC13660.2297984301032579No Hit
CTCTAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA13330.22424693069373558No Hit
ATTATCAACAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATGAAAA13260.2230693399098975No Hit
GGATATTATCAACAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATG12990.2185272040293792No Hit
ATTTACATACTTTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACA12620.21230279560052082No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCATTAAC12020.20220916031048025No Hit
ATATGTTCCTGTGGCAAAGTACCCCAACTTCCAATTACATATCCCATGAA12000.20187270580081218No Hit
CTCCAAAAGACACCAAATACTCTAGAACAGTTTCTCTTCCAAAAGTAAGA11790.198339933449298No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT11410.1919472977656056No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTGCCACAG10710.1801713899272249No Hit
ATGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCAGGAT10700.1800031626723909No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG10480.17630216306604266No Hit
GTATACATGCATATAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAA10410.1751245722822046No Hit
GTTCTATCCTAACCTTACCAAATATTTACCCTTAGATAAAGGCATTAAAC10310.1734422997338645No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAATTATT10290.17310584522419645No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT10070.16940484561784824No Hit
ATTTTGTGGTCTGTAAGCGGGAGGAGTGCGAATCCACACTCCAAAAGACA10010.16839548208884417No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC9910.1667132095405041No Hit
CTTTTACACAATGTGGTTACCCAGCATTAATGCCTTTATATGCATGTATA9880.16620852777600204No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGTAAATATTTGGTAAGGTT9870.16604030052116805No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA9870.16604030052116805No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG9780.16452625522766193No Hit
ATCTAAGGGTAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAA9660.16250752816965383No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAAT9450.1589747558181396No Hit
GGAACATATTGTACAAAAATTGAAACAATGTTTTCGGAAACTTCCTATAA9200.15476907444728935No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC9110.15325502915378325No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC8920.15005871131193707No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC8860.149049347782933No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT8840.14871289327326498No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG8590.14450721190241472No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT8570.1441707573927467No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG8540.14366607562824468No Hit
ATGTTAATATGGGCCTAAAAATCAGACAATTATTGTGGTTTCACATTTCC8480.14265671209924063No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT8390.14114266680573453No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGACTCCCTTTTTACC8340.14030153053156447No Hit
GTTGATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCC8320.13996507602189645No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC8320.13996507602189645No Hit
GCTTACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC7790.13104903151569391No Hit
AGTCTGTACAACATCTTGACTCCCTTTTTACCGCTATTACCAATTTTCTT7700.12953498622218781No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7640.12852562269318377No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGCGGTAAAAA7470.12566575936100557No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG7430.12499285034166954No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG7350.12364703230299746No Hit
CTTATATAAAATACCAGCCTTCCACAAAGTATGTAAATAGTGTCTAGTTT7300.12280589602882742No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC7270.12230121426432539No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG7250.12196475975465737No Hit
CCCTTAGATAAAGGCATTAAACCTTATTATCCTGAACATACAGTTAATCA7180.1207871689708193No Hit
CTCTTATATAAAATACCAGCCTTCCACAAAGTATGTAAATAGTGTCTAGT7100.11944135093214722No Hit
GCTTTGGGGCATGGACATTGACCCATATAAAGAATTTGGAGCTTCTGTGG7010.11792730563864114No Hit
TCCTCAGGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCTCTGCTAG7010.11792730563864114No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6980.1174226238741391No Hit
TTATTATCCTGAACATACAGTTAATCATTACTTCCAAACTAGACACTATT6970.11725439661930508No Hit
GTACAACATCTTGACTCCCTTTTTACCGCTATTACCAATTTTCTTTTGTC6750.11355339701295687No Hit
CTATTTACATACTTTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTA6660.11203935171945076No Hit
GTCTTACATAAGAGGACTCTTGGACTTTCAGCAATGTCAACGACCGACCT6650.11187112446461675No Hit
CTTTAGGGCATGGACATTGACCCATATAAAGAATTTGGAGCTTCTGTGGA6550.11018885191627667No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAC6520.10968417015177463No Hit
GGGTAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAATCAATT6470.10884303387760459No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGTAAATATTTGGTA6450.10850657936793656No Hit
ATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCCAGTC6400.10766544309376651No Hit
AAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATGTAATTGGA6350.10682430681959645No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6350.10682430681959645No Hit
GTTAATATGGGCCTAAAAATCAGACAATTATTGTGGTTTCACATTTCCTG6290.10581494329059239No Hit
GTTCTAGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCCCGC6240.10497380701642235No Hit
GAATATGGTGACCCACAAAATGAGGCACTGCGTGTAGTTTCTCTCTTATA6180.10396444348741829No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACAC6150.10345976172291625No Hit
CAATAGGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGT6140.10329153446808224No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC6020.10127280741007412No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTATAGGAAGTTTCCGAAAAC6020.10127280741007412No Hit
TATTAACATTGACATAGCTTACTACTAATTCCCTGGATGCTGGGTCTTCC5960.10026344388107006No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)