FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488041

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488041
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences594434
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN38950.6552451575784696No Hit
GTATGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCAGG24230.40761463846280666No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG17180.28901442380482945No Hit
ATCCTAACCTTACCAAATATTTACCCTTAGATAAAGGCATTAAACCTTAT16550.27841610675028683No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCA16330.2747151071439386No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG14870.25015392793817315No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA14770.248471655389833No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC14500.24392951950931474No Hit
GGATATTATCAACAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATG14040.2361910657869503No Hit
GTGTAAAAGGGGCGGCAAAGCCCAGAAGACCCACAATTCTTTGACATACT14030.23602283853211628No Hit
CTCTAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA13920.23417233872894214No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAATATGTT13550.22794793030008376No Hit
CTGTAAGAGGGCCTACAAATTGTTGACATCTGTTGATAATATCCTCTTTC13370.22491983971307158No Hit
ATTATCAACAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATGAAAA13300.2237422489292335No Hit
ATTTACATACTTTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACA12630.21247102285535485No Hit
ATATGTTCCTGTGGCAAAGTACCCCAACTTCCAATTACATATCCCATGAA12350.20776065972000257No Hit
CTCCAAAAGACACCAAATACTCTAGAACAGTTTCTCTTCCAAAAGTAAGA12020.20220916031048025No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT11480.19312488854944368No Hit
ATGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCAGGAT11390.19161084325593758No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCATTAAC11350.19093793423660155No Hit
CTTTTACACAATGTGGTTACCCAGCATTAATGCCTTTATATGCATGTATA11050.18589111659158122No Hit
GTATACATGCATATAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAA10660.17933025365305483No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT10350.17411520875320052No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGTAAATATTTGGTAAGGTT10250.17243293620486042No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAATTATT10230.1720964816951924No Hit
ATCTAAGGGTAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAA9980.16789080032434214No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTGCCACAG9880.16620852777600204No Hit
GTTCTATCCTAACCTTACCAAATATTTACCCTTAGATAAAGGCATTAAAC9860.16587207326633402No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG9810.16503093699216398No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA9590.16132993738581575No Hit
ATTTTGTGGTCTGTAAGCGGGAGGAGTGCGAATCCACACTCCAAAAGACA9440.1588065285633056No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAAT9350.1572924832697995No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG9150.1539279381731193No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC9070.15258212013444722No Hit
GGAACATATTGTACAAAAATTGAAACAATGTTTTCGGAAACTTCCTATAA8980.15106807484094115No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT8850.148881120528099No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC8850.148881120528099No Hit
ATGTTAATATGGGCCTAAAAATCAGACAATTATTGTGGTTTCACATTTCC8830.148544666018431No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG8590.14450721190241472No Hit
GTTGATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCC8580.14433898464758071No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC8470.1424884848444066No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGCGGTAAAAA8440.1419838030799046No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC8380.14097443955090053No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG7950.1337406675930381No Hit
CTCTTATATAAAATACCAGCCTTCCACAAAGTATGTAAATAGTGTCTAGT7910.13306775857370204No Hit
CTTATATAAAATACCAGCCTTCCACAAAGTATGTAAATAGTGTCTAGTTT7700.12953498622218781No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT7700.12953498622218781No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGACTCCCTTTTTACC7670.12903030445768582No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG7580.1275162591641797No Hit
GCTTTGGGGCATGGACATTGACCCATATAAAGAATTTGGAGCTTCTGTGG7450.1253293048513376No Hit
AGTCTGTACAACATCTTGACTCCCTTTTTACCGCTATTACCAATTTTCTT7370.1239834868126655No Hit
GTCTTACATAAGAGGACTCTTGGACTTTCAGCAATGTCAACGACCGACCT7310.12297412328366143No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7240.12179653249982335No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG7170.12061894171598529No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG7120.11977780544181524No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG7070.11893666916764518No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT7010.11792730563864114No Hit
TTATTATCCTGAACATACAGTTAATCATTACTTCCAAACTAGACACTATT6960.11708616936447107No Hit
CTTTAGGGCATGGACATTGACCCATATAAAGAATTTGGAGCTTCTGTGGA6950.11691794210963707No Hit
CTATTTACATACTTTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTA6940.11674971485480307No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC6850.11523566956129695No Hit
CCCTTAGATAAAGGCATTAAACCTTATTATCCTGAACATACAGTTAATCA6830.11489921505162895No Hit
TCCTCAGGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCTCTGCTAG6820.11473098779679494No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC6780.11405807877745891No Hit
GTTCTAGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCCCGC6710.11288048799362083No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6590.1108617609356127No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGTAAATATTTGGTA6500.1093477156421066No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAC6430.10817012485826855No Hit
GTTAATATGGGCCTAAAAATCAGACAATTATTGTGGTTTCACATTTCCTG6410.10783367034860052No Hit
GCTTACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC6400.10766544309376651No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA6350.10682430681959645No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGTCCGACCACG6240.10497380701642235No Hit
GTACAACATCTTGACTCCCTTTTTACCGCTATTACCAATTTTCTTTTGTC6150.10345976172291625No Hit
CCTCTATGTTTCCCTCTTGCTGCTGTACAAAACCTTCGGACGCAAATTGC6140.10329153446808224No Hit
GGGTAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAATCAATT6110.1027868527035802No Hit
CAATAGGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGT6100.1026186254487462No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTCTGG6100.1026186254487462No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA6080.10228217093907817No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG6040.10160926191974214No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5960.10026344388107006No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)