FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485156
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACCGTATGTTCAGG20030.4128568955140202No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18750.38647362910074284No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT16300.33597440823157915No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA14540.29969741691332275No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC12700.26177147144423646No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG12350.254557297034356No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA12080.24899207677530527No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG11810.24342685651625456No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11380.2345637279555442No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTGTTGATAATATCCTCTTTC10750.22157821401775926No Hit
CTCTAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATATGAAACCACA10030.2067376266602907No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT9220.19004196588313862No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAATATGTT9160.18880525027001624No Hit
CTCCAAAAGACACCAAATACTCTAGAACAGTTTCTCTTCCAAAAGTAAGA8830.18200331439784315No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC8500.1752013785256701No Hit
GTGTAAAAGGGGCAGCAAAGCCCAGAAGACCCACAATTCTTTGACATACC8460.17437690145025517No Hit
GGATATTATCAACAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG8410.17334630510598653No Hit
ACCACGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC8400.1731401858371328No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGCTGGGGTACCTTACCACAG8350.17210958949286415No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTTTCCATGC8180.16860556192235074No Hit
GTATACATGCATATAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAA7910.16304034166330006No Hit
ATATGTTCCTGTGGTAAGGTACCCCAGCTTCCAATTACATATCCCATGAA7830.1613913875124702No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG7630.15726900213539563No Hit
ATTATCAACAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAA7580.15623840579112697No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7560.15582616725341952No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT7330.15108542406978373No Hit
TCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACCGTA7330.15108542406978373No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATCCCTTGGACTCATAAGGTG7260.14964258918780765No Hit
CCTATAAATAGACCTATTGATTGGAAGGTATGTCAAAGAATTGTGGGTCT7250.1494364699189539No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG7140.14716915796156288No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACA7130.14696303869270916No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG7060.14552020381073305No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC7010.14448960746646441No Hit
GAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACCGTATGTTCA6870.14160393770251217No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT6870.14160393770251217No Hit
CCTAACGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6850.1411916991648047No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC6770.1395427450139749No Hit
GTTTGGGACTCTACCGTCCTCTTCTCCGTCTGCCGTTCCGACCGACCACG6600.13603871744346147No Hit
CTTTTACACAATGTGGTTACCCAGCATTAATGCCTTTATATGCATGTATA6580.13562647890575402No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTT6570.1354203596369003No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA6550.13500812109919283No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC6540.1348020018303391No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6430.13253468987294809No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA6320.13026737791555706No Hit
GCATTGCTGGAGTTCCACTGCATGGCCTGAGGATGACTGTCTCTTAGAGG6240.1286184237647272No Hit
TTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC6210.12800006595816601No Hit
GGAACATATTGTACAAAAATTGAAACAATGTTTTCGGAAACTTCCTATAA6130.1263511118073362No Hit
GTTGATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCC6110.12593887326962871No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCGTCTATTCG6030.1242899191187989No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6000.12367156131223771No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG5930.12222872643026161No Hit
GTTCTAGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTAACGC5820.11996141447287059No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5780.11913693739745566No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGT5780.11913693739745566No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA5780.11913693739745566No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG5710.11769410251547954No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA5690.1172818639777721No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5690.1172818639777721No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAAGCCCTTTTTACC5590.1152206712892348No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAAT5580.11501455202038108No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5550.11439619421381989No Hit
GCATTAATGCCTTTATATGCATGTATACAAGCTAAGCAGGCTTTCACTTT5520.1137778364072587No Hit
GCACGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATG5500.11336559786955124No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5500.11336559786955124No Hit
ATGTAATTGGAAGCTGGGGTACCTTACCACAGGAACATATTGTACAAAAA5490.11315947860069751No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA5490.11315947860069751No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC5450.11233500152528259No Hit
ACCCATCTCGGGGCCGTTTGGGACTCTACCGTCCTCTTCTCCGTCTGCCG5420.1117166437187214No Hit
ATGCTGGGTCGTCCAAATTACTTCCCACCCAGGTGGCCAGATTCATCAAC5410.11151052444986767No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5400.11130440518101394No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC5390.11109828591216021No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC5340.11006768956789156No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5300.10924321249247665No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA5260.10841873541706172No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA5240.10800649687935428No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5170.10656366199737816No Hit
TTATTATCCTGAACATACGGTTAATCATTACTTCCAAACTAGACATTATT5170.10656366199737816No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC5160.10635754272852443No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC5140.10594530419081698No Hit
ACCCAGCATCCAGGGAATTAGTAGTAAGCTATGTCAACGTTAACATGGGC5130.10573918492196326No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCCCGAA5110.10532694638425578No Hit
CCTCTCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5050.10409023077113341No Hit
AGTCTGTACAACATCTTGAAGCCCTTTTTACCTCTATTACCAATTTTCTT5030.10367799223342596No Hit
GACAACTATTGTGGTTTCATATTTCCTGTCTTACTTTTGGAAGAGAAACT5020.1034718729645722No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCATCTCGGGGCCG4990.10285351515801104No Hit
ATCTAAATCTCGGGAATCTCAATGTTAGTATCCCTTGGACTCATAAGGTG4960.10223515735144983No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAAACC4880.10058620320062002No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC4860.10017396466291255No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)