FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428878
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN79801.860669001440969No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13470.3140753314462388No Hit
ATATGATCCTGTGGCAATGTACCCCAACTTCCAATTACATAACCCATAAA10530.2455243682352557No Hit
GTATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAG10260.23922887161383888No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9230.21521271783584142No Hit
GGATCATATTGTACAAAAAATCAAACACTGTTTTAGAAAACTTCCTGTCA9000.20984988738056046No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATGATTAACTA8670.20215539150993989No Hit
GTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA8440.19679256105465892No Hit
GTGTAAATGGAGCGGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT8250.19236239676551373No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTACTCCCTAAATTTTAT8030.1872327328517667No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA7730.18023773660574804No Hit
GGGCTGTATCGTCCCCTTCTCCGTCTGCCGTACCGTCCGACCACGGGGCG7250.16904574261211813No Hit
CTTATATAGAATCCCAGCCTTCCAAAGAGTATGCAAATAATGTCGGGTTT7050.164382411781439No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCCGTCCG6960.16228391290763342No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAGTACCACATC6790.15832008170155615No Hit
CCATTTACACAATGTGGGTACCCTGCATTAATGCCTTTGTATGCATGTAT6430.14992608620633371No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA6290.14666175462485836No Hit
GTATACATGCATACAAAGGCATTAATGCAGGGTACCCACATTGTGTAAAT6200.14456325575105275No Hit
CTCTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGCCTTGGATGGC5640.13150592942515119No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5370.1252104328037344No Hit
GTACCCACATTGTGTAAATGGAGCGGCAAAGCCCAAAAGACCCACAATTC5370.1252104328037344No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG5250.12241243430532692No Hit
ATACAGCCCCAAGCGGCCTCGCGAGGGGTCGTCCGCGGGATTCAGCGCCG5250.12241243430532692No Hit
GTTTTAGAAAACTTCCTGTCAATCGACCTATTGATTGGAAAGTATGTCAG5170.12054710197305528No Hit
GTACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA5160.12031393543152133No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG5150.12008076888998737No Hit
CCTATTAGCAATGTCTTCATGTAAATGAATTTTAGGAAAAGAAGGAGTTT5110.11914810272385154No Hit
GGGTAAAGGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA4990.11635010422544406No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG4990.11635010422544406No Hit
CTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGCCTTGGATGGCTT4840.11285260610243472No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4750.11075410722862912No Hit
TTCTTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACTCCTTCTTTTC4740.11052094068709517No Hit
ATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAGTG4730.11028777414556121No Hit
ATATAGAGCTGAGGCTGTATCTAGTAGATCCCGGACGGAAGGAAAGAAGT4690.10935510797942538No Hit
ATGTGGTACTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACC4690.10935510797942538No Hit
CTCCGAAAGAGACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGG4650.10842244181328957No Hit
GGATAATAAGGTTTAATCCCTTTGTCTAAAGGCAAATATTTTGTGGTGTT4600.10725660910561978No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG4540.10585760985641604No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT4520.10539127677334813No Hit
TTCTAAAACAGTGTTTGATTTTTTGTACAATATGATCCTGTGGCAATGTA4500.10492494369028023No Hit
GGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA4490.10469177714874625No Hit
GCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGTAAATGAAAAAAG4440.10352594444107649No Hit
CTGCCGTACCGTCCGACCACGGGGCGCACCTCTCTTTACGCGGTCTCCCC4410.10282644481647461No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)