FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences556501
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT30070.5403404486245308No Hit
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG29260.5257852187147912No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20380.36621677229690514No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT19700.3539975669405805No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC19690.353817872744164No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA18150.3261449664960171No Hit
ATATGATCCTGTGGCAAAGTTCCCCAACTTCCAATTATGTAACCCATGAA16470.2959563414980387No Hit
ATTCTATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAAC16240.2918233749804583No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT15880.2853543839094629No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA15570.2797838638205502No Hit
AGTCTGTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT15480.27816661605280135No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT15440.2774478392671352No Hit
TATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT15330.27547120310655326No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC15170.2725960959638887No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT15100.2713382365889729No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT14900.2677443526606421No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC14720.26450985712514447No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA14720.26450985712514447No Hit
CTTCAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG14340.257681477661316No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC13890.24959523882257173No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGGT13660.24546227230499137No Hit
TTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAG13470.24204808257307717No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG13230.2377354218590802No Hit
TTCAAGTACAGTCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACA12780.229649183020336No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12540.2253365223063391No Hit
CTCCGAAAGAGACCAAATATTCAAGTACAGTCTCTCTTCCAAAAGTAAGG12260.220305084806676No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG12230.2197660022174264No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC12220.2195863080210098No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA11900.2138360937356806No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT11540.20736710266468522No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT11440.20557016070051987No Hit
GGGTAAAGGTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA10640.19119462498719678No Hit
GTACAATATGATCCTGTGGCAAAGTTCCCCAACTTCCAATTATGTAACCC10070.1809520557914541No Hit
ATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGG10030.18023327900578795No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA9830.17663939507745718No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG9750.1752018415061249No Hit
GTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA9750.1752018415061249No Hit
CTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCATTTACAAG9640.17322520534554298No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTAT9550.1716079575777941No Hit
TTCTAAAACAGTGTTTGATCTTTTGTACAATATGATCCTGTGGCAAAGTT9500.17070948659571142No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCAG9460.16999070981004527No Hit
GCTTTATTCCTCTTCAGTACCTATCTTTAATCCTGAATGGCAAACTCCTT9160.16459988391754912No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8930.1604669173999687No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC8860.15920905802505297No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8720.15669333927522144No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTGTTTGATCTTTTGT8710.15651364507880489No Hit
ATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTAT8520.15309945534689068No Hit
CCCTTAGACAAAGGAATTAAACCTTATTATCCAGATCAGGTAGTTAATCA8280.14878679463289374No Hit
CTCCTAGACACCGCCTCAGCTCTGTATCGAGAAGCCTTAGAGTCTCCTGA8280.14878679463289374No Hit
GTCTAAGGGCAAATATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAA8220.1477086294543945No Hit
GTCTAGGAGATCTCTGACGGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA8190.14716954686514488No Hit
TTGACATACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC8120.14591168749022915No Hit
GGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCA7660.13764575445506835No Hit
ATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAG7620.1369269776694022No Hit
GTATTAACATAATTGACTACTAGATCCCTGGATGCTGGATCTTCCAAATT7510.1349503415088203No Hit
GTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC7430.13351278793748797No Hit
GTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGGAAAGTATGTCAA7350.13207523436615567No Hit
CTGTAAATGAAAAGAGAAGATTGAAATTAATTATGCCTGCTAGATTCTAT7340.13189554016973914No Hit
CTCTAAGGCTTCTCGATACAGAGCTGAGGCGGTGTCTAGGAGATCTCTGA7030.12632502008082647No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTGCACTCAGGCA7000.12578593749157685No Hit
ATGTTAATACTAACATGGGTTTAAAGATCAGGCAACTATTGTGGTTTCAT6830.12273113615249569No Hit
GGTTTAAAGATCAGGCAACTATTGTGGTTTCATATATCTTGCCTTACTTT6800.12219205356324607No Hit
GTATAAAGGGACTCACGATGCTGTACAGACTTGGCCCCCAATACCACATC6770.12165297097399645No Hit
ATACTGCACTCAGGCAAGCCATTCTCTGTTGGGGAGAATTGATGACTCTA6750.12129358258116338No Hit
TGTTAATACTAACATGGGTTTAAAGATCAGGCAACTATTGTGGTTTCATA6610.11877786383133185No Hit
GGATATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAAT6550.11769969865283261No Hit
CTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC6490.11662153347433338No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAA6470.11626214508150032No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCCTCTTCAGTACCTATC6450.11590275668866723No Hit
GTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAACAG6420.11536367409941761No Hit
GAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCT6420.11536367409941761No Hit
GTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG6330.11374642633166876No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG6290.1130276495460026No Hit
GATCTGGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTG6260.11248856695675301No Hit
GTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGGCCTAT6230.11194948436750339No Hit
CTTTAAACCCATGTTAGTATTAACATAATTGACTACTAGATCCCTGGATG6200.11141040177825377No Hit
ATTTACAAGAGGACATTATTAATAGGTGTCAACAATTTGTGGGCCCTCTC6200.11141040177825377No Hit
ATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAAC6180.11105101338542069No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTC6170.11087131918900416No Hit
TCCTAATACAAAGACCTTTAACCTAATCTCCTCCCCCAGCTCCTCCCAGT6080.10925407142125532No Hit
ATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATATT6060.10889468302842224No Hit
AGATATATGAAACCACAATAGTTGCCTGATCTTTAAACCCATGTTAGTAT6000.107816517849923No Hit
GTGTTTGATCTTTTGTACAATATGATCCTGTGGCAAAGTTCCCCAACTTC5940.10673835267142376No Hit
CATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAATGGAGC5930.10655865847500723No Hit
TCACCATACTGCACTCAGGCAAGCCATTCTCTGTTGGGGAGAATTGATGA5920.10637896427859068No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCCTCTTCAG5900.10601957588575762No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA5890.10583988168934108No Hit
GTATCGAGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTG5850.10512110490367493No Hit
GACCAATTTATGCCTACAGCCTCCTAATACAAAGACCTTTAACCTAATCT5830.10476171651084185No Hit
TCTCGATACAGAGCTGAGGCGGTGTCTAGGAGATCTCTGACGGAAGGAAA5770.10368355133234261No Hit
CTTTTGTACAATATGATCCTGTGGCAAAGTTCCCCAACTTCCAATTATGT5730.10296477454667646No Hit
TCCCTAAACTTCATGGGTTACATAATTGGAAGTTGGGGAACTTTGCCACA5600.10062874999326146No Hit
CTCTCACTGTAAATGAAAAGAGAAGATTGAAATTAATTATGCCTGCTAGA5570.10008966740401185No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)