FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449072
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG18280.4070616738518545No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT17430.3881337513806249No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT14930.3324633911711262No Hit
GTCTTTGAAGTAAGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA13550.301733352335483No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG12530.27901984537000746No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC12530.27901984537000746No Hit
TATTTAGTGTGGGTAGGATAAAATCTAGCAGGCATAATTAATTTCAATCT12070.26877649909145973No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT12020.2676630918872697No Hit
CTCCAAAAGAGACCAAATATTCAAGTACAGTCTCTCTTCCAAAAGTAAGG11950.2661043218014038No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11710.2607599672212919No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC11330.25229807246944813No Hit
ATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA11220.24984857662023016No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11150.2482898065343642No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC10950.24383617771760432No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC10620.23648769016995047No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG10440.23247942423486656No Hit
TTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAG10410.23181137991235257No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCTTAAATTTCAT9980.22223607795631878No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9710.21622367905369294No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA9630.21444222752698897No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9600.21377418320447503No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA9560.21288345744112305No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG9530.21221541311860903No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCAG9330.20776178430184913No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA9310.20731642142017315No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG9160.2039761998076032No Hit
CTCCTAGACACCGCCTCAGCTCTGTATCGAGAAGCCTTAGAGTCTCCTGA8890.19796380090497737No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGGT8640.19239676488402752No Hit
AGTCTGTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT8330.18549364021804968No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC8290.1846029144546977No Hit
GTATACATGCATACAAGGGCATTAAGGCAGGATATCCACATTGTGTAAAT8030.17881319699290982No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC7970.17747710834788186No Hit
GTCTTACATAAGAGGACTCTTGGACTCCCAGCAATGTCAACGACCGACCT7970.17747710834788186No Hit
ATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATATT7820.17413688673531194No Hit
GTCTAAGGGCAAATATTTAGTGTGGGTAGGATAAAATCTAGCAGGCATAA7540.16790180639184807No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCTTAA7370.16411622189760217No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAA7270.16188940748922223No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT7130.1587718673174903No Hit
TTCAAGTACAGTCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACA7120.15854918587665232No Hit
GTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG6920.1540955570598924No Hit
GTATAAAGGGACTCACGATGCTGTACAGACTTGGCCCCCAATACCACATC6710.14941924680229451No Hit
CTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC6650.14808315815726655No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6610.14719243239391455No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG6600.14696975095307657No Hit
CCCTTAGACAAAGGAATTAAACCTTATTATCCAGATCAGGTAGTTAATCA6570.14630170663056258No Hit
GTCTAGGAGATCTCTGACGGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA6430.14318416645883067No Hit
GTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCC6430.14318416645883067No Hit
GTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA6200.13806249331955678No Hit
TTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTC6170.13739444899704278No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTGCACTCAGGCA6010.13383154594363486No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT6010.13383154594363486No Hit
TTCTAAAACAGTGTTTGATCTTTTGTACAATATGATCCTGTGGCAATGTT5970.13294082018028291No Hit
ATTTTATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAAC5740.12781914704100902No Hit
ATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGG5650.12581501407346707No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCCTTGTATGCATGTAT5640.12559233263262906No Hit
GGATAAAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC5570.1240335625467631No Hit
GGGTAAAGGTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA5520.12292015534257313No Hit
TCCTAATACAAAGACCTTTAACCTAATCTCCTCCCCCAGCTCCTCCCAGT5480.12202942957922115No Hit
TCCTCAGGCCATGCAGTGGAATTCCACTGCCTTCCACCAAGCTCTGCAGG5450.12136138525670717No Hit
CTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCATTTACAAG5410.1204706594933552No Hit
GTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGGAAAGTATGTCAA5350.11913457084832722No Hit
GTATCGAGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTG5340.11891188940748923No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG5310.11824384508497524No Hit
CTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTTTTGCCTTCT5300.11802116364413726No Hit
CCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCT5290.11779848220329925No Hit
GTATTAACATAATTGACTACTAGATCCCTGGATGCTGGATCTTCCAAATT5260.11713043788078527No Hit
GCCTAATCATCTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGC5220.11623971211743328No Hit
GTATATTTCCGCGAGAGGACGACAGAATTGTCAGTTCCGATGAGCTTTGC5190.11557166779491931No Hit
CCCATAGGTATTTTGCGAAAGCCCAGGACGATGGGATGGGAATACAGGTG5180.1153489863540813No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC5160.1149036234724053No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTCCGCGAGAGGACGACAGAAT5080.11312217194570137No Hit
GGTTTAAAGATCAGGCAACTATTGTGGTTTCATATATCTTGCCTTACTTT4970.1106726760964834No Hit
CTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTT4970.1106726760964834No Hit
CTTTAAACCCATGTTAGTATTAACATAATTGACTACTAGATCCCTGGATG4920.10955926889229343No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4910.10933658745145546No Hit
ATACTGCACTCAGGCAAGCCATTCTCTGCTGGGGGGAATTGATGACTCTA4820.1073324544839135No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGCCT4730.10532832151637153No Hit
GTACTGTAGAGGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAA4730.10532832151637153No Hit
ATGTATATTTCCGCGAGAGGACGACAGAATTGTCAGTTCCGATGAGCTTT4710.10488295863469554No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCCTCTACAG4690.10443759575301957No Hit
CTCTTTGGAAGGCTGGTATTCTATATAAGAGGGAAACCACACGTAGCGCA4640.10332418854882958No Hit
CATATATCTTGCCTTACTTTTGGAAGAGAGACTGTACTTGAATATTTGGT4620.10287882566715359No Hit
CTCTAAGGCTTCTCGATACAGAGCTGAGGCGGTGTCTAGGAGATCTCTGA4590.10221078134463961No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGG4580.1019880999038016No Hit
TCACCATACTGCACTCAGGCAAGCCATTCTCTGCTGGGGGGAATTGATGA4580.1019880999038016No Hit
GTGGATATCCTGCCTTAATGCCCTTGTATGCATGTATACAAGCTAAACAG4570.10176541846296362No Hit
ATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTTGGA4570.10176541846296362No Hit
TCTCGATACAGAGCTGAGGCGGTGTCTAGGAGATCTCTGACGGAAGGAAA4500.10020664837709767No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)