FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488051

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488051
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences408475
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA26290.6436134402350204No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13860.33931085133729116No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT12250.2998959544647775No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA10130.24799559336556704No Hit
CTGTAATGTCAACGACCGACCTTGAGGCCTACTTCAAAGACTGTTTGTTT9730.23820307240345187No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG9710.2377134463553461No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC9390.22987942958565397No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT9320.2281657384172838No Hit
TCCAAATTCCAGGGACCTAGTAGTTAGTTATGTTAACACTAATATGGGCC9090.22253503886406756No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT9030.22106616071975027No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8890.21763877838301No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC8750.21421139604626965No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG7980.19536079319419794No Hit
CTATAATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC7940.19438154109798642No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT7750.1897300936409817No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT7740.18948528061692882No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT7580.18556827223208275No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT7560.18507864618397699No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTACG7490.18336495501560685No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7440.18214088989534244No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT7010.1716139298610686No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT6880.16843136054838118No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTCAT6500.15912846563437175No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC6240.1527633270089969No Hit
CTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCT6160.15080482281657384No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA6160.15080482281657384No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT6080.14884631862415082No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT5990.1466430014076749No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5960.14590856233551625No Hit
CTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC5920.14492931023930472No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5870.14370524511904031No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG5840.1429708060468817No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC5680.13905379766203563No Hit
ATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTCACGGTGGTCTCCATGC5590.1368504804455597No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5580.13660566742150682No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTC5570.13636085439745396No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT5470.13391272415692515No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTAC5390.13195421996450213No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT5330.13048534182018484No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGA5310.12999571577207908No Hit
TCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGAGC5220.12779239855560315No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA5150.126078707387233No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT5140.12583389436318013No Hit
ATCCAATATCCAGGGACCTAGTAGTTAGTTATGTTAACACTAATATGGGC5090.12460982924291572No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA5010.1226513250504927No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5000.12240651202643982No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTCT4960.12142725993022829No Hit
ACATTATAGAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGG4920.12044800783401677No Hit
AGCTGGAGGAGTGCGGATCCACACTCCGAAAGACACCAAATACTCTATGA4850.11873431666564661No Hit
GTGAAAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAA4830.11824469061754085No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT4810.11775506456943509No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT4800.11751025154538221No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTCACCATATT4770.11677581247322356No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA4710.1153069343289063No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC4710.1153069343289063No Hit
GCTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG4710.1153069343289063No Hit
GTAGAAGAGTAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT4690.11481730828080054No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAG4670.11432768223269478No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA4530.11090029989595446No Hit
ATATTAGTGTTAACATAACTAACTACTAGGTCCCTGGAATTTGGATCGTC4480.10967623477569005No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC4450.10894179570353144No Hit
CTCCGAAAGACACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGA4450.10894179570353144No Hit
GATCATTAACCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC4430.10845216965542567No Hit
AGCTTGAGGAGTGCGGATCCACACTCCGAAAGACACCAAATACTCTATGA4400.10771773058326703No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4380.10722810453516127No Hit
ATCTAGAAGATCTCGTACGGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA4360.1067384784870555No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4360.1067384784870555No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4330.10600403941489689No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC4330.10600403941489689No Hit
AGTCTAGACTCTTCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4310.10551441336679113No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTACTCTTCTACTG4250.10404553522247384No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC4220.1033110961503152No Hit
GTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCC4220.1033110961503152No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT4210.10306628312626231No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAA4190.10257665707815657No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG4160.10184221800599792No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG4120.1008629659097864No Hit
ACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTT4100.10037333986168064No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4100.10037333986168064No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC4090.10012852683762775No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)