FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences508838
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18240.35846379397765105No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA17280.3395972785051431No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT17240.3388111736937886No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT16100.3164071865701854No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC15680.3081530860509632No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC14740.28967962298413247No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG12950.2545014326760187No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG11420.22443292364170914No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG10530.2069420915890716No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC10500.2063525129805557No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA9970.1959366242301086No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG9850.19357830979604512No Hit
CTGTAAGAGGGCCCACAAATTGTTGACATCTGTTGATAATATCCTCTTTC9670.19004083814494988No Hit
GGATATTATCAACAGATGTCAACAATTTGTGGGCCCTCTTACAGTTAACG9630.18925473333359538No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA9570.18807557611656361No Hit
GCCTAGCAGCCATGGAAAAGAGGTGTATTTCCGAGAGAGGACAACAGAGT9410.18493115687114564No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA9180.1804110542058573No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG9140.17962494939450277No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8950.17589095154056889No Hit
CATTTAAACCCCAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT8630.1696021130497329No Hit
GGAACATATTGTACAAAAAATCAAACAATGTTTTAGGAAACTTCCTATCA8090.1589896980964472No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG8080.15879317189360856No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC8060.1584001194879313No Hit
ATGTAAGACCTTAGGCAAGACTTGGCGGGCGTTCACGGTGGTCTCCATGC8020.15761401467657685No Hit
CCCAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTC7870.15466612163399746No Hit
CAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGG7780.15289738580844983No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTT7740.15211128099709534No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG7690.15112864998290224No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTAAC7560.1485738093460001No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7390.14523286389774348No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCTCCTCACCATACAG7370.14483981149206623No Hit
CCCTTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAATACACAGTCTTT7330.14405370668071174No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG7260.14267802326084136No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT7240.14228497085516412No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA7230.14208844465232548No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT7110.139730130218262No Hit
CTTTAATCCTGAGTGGCATACTCCCTCTTTTCCTCACATTCATTTGAAAG7060.13874749920406887No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG6980.13717528958135988No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCACGAATAGAAGGAAA6890.13540655375581226No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC6820.1340308703359419No Hit
ATTATCAACAGATGTCAACAATTTGTGGGCCCTCTTACAGTTAACGAAAA6820.1340308703359419No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT6690.13147602969903976No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT6570.12911771526497628No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTA6500.1277420318451059No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6480.12734897943942866No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC6410.12597329601955828No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6410.12597329601955828No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG6380.1253837174110424No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA6300.12381150778833343No Hit
GCTTACTACTAATTCCCTGGATGCCGGGTCTTCCAAATTACTTCCCACCC6290.1236149815854948No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCGCCAAGTCTTGCCT6230.12243582436846305No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC6180.12145319335426993No Hit
GTACAAAACCTTCGGACGGAAATTGCACTTGTATTCCCATCCCATCATCT6140.12066708854291543No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTGGGGTTTAAATGTATA6130.1204705623400768No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC6080.11948793132588369No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCATACTCCCTCTTTTCCTCAC6070.11929140512304506No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA6060.11909487892020643No Hit
ATTGACAACAGTACCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT6040.11870182651452918No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG5970.11732614309465882No Hit
TCCTCAGGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCTCTGCTAG5910.11614698587762706No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTAGGCAAGACTTGGCGGGCGTTC5900.11595045967478844No Hit
CTTCAAAGACTGTGTATTTAAGGACTGGGAGGAGTTGGGGGAGGAGATTA5890.11575393347194982No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG5780.11359214524072495No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCACGAA5760.1131990928350477No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA5730.11260951422653181No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACCTTT5640.11084077840098422No Hit
GCCTAATCATCTCATGTTCATGTTCCACTGTTCAAGCCTCCAAGCTGTGC5600.1100546735896297No Hit
GGTCTATTGATAGGAAGTTTCCTAAAACATTGTTTGATTTTTTGTACAAT5570.10946509498111383No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC5420.10651720193853448No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5420.10651720193853448No Hit
GAATTAGTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACA5380.10573109712717996No Hit
ATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG5350.1051415185186641No Hit
TATTAACATTGACATAGCTTACTACTAATTCCCTGGATGCCGGGTCTTCC5270.1035693088959551No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5240.10297973028743923No Hit
CTACTAATTCCCTGGATGCCGGGTCTTCCAAATTACTTCCCACCCAGGTG5210.10239015167892336No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5200.10219362547608471No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5190.1019970992732461No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5170.10160404686756885No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5130.10081794205621436No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT5120.10062141585337574No Hit
TAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCT5110.10042488965053711No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT5110.10042488965053711No Hit
GTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTG5090.10003183724485985No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)