FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488054

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488054
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323310
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT16570.5125112121493304No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG16490.5100368067798707No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC14990.4636417061025022No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA14580.4509603785840215No Hit
ACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACAAAACC13930.43085583495716184No Hit
GGAACATATTGTACAAAAAATCAAACTGTGTTTTAGAAAAATTCCTGTAA13290.41106059200148465No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT12430.3844607342797934No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA12420.3841514336086109No Hit
GTTTACTTAGAAAGGCCTTGTAAATTGGCGAGAAAGTAAAAGCCTGTTTA12200.37734681884259685No Hit
GTCCCGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC11170.3454888497108039No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT10510.32507500541276174No Hit
GTCTCAGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTGTACCGAA10330.31950759333147755No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAATTGGCGA10160.31424948192137575No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG9930.3071355664841793No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9830.3040425597723547No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG9560.2956914416504284No Hit
CTAAAACACAGTTTGATTTTTTGTACAATATGTTCCTGTGGCAATGTGCC9490.2935263369521512No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC9270.2867217221861371No Hit
GAGCAGTAGTCGTGCAGGTTTTGCATGGTCCCGTGCTGGTTGTTGATGAT9260.2864124215149547No Hit
GAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCAC8790.2718752899693792No Hit
CAATAGGCCTATTTACAGGAATTTTTCTAAAACACAGTTTGATTTTTTGT8600.26599857721691256No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG8520.2635241718474529No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA8490.26259626983390555No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT8480.26228696916272304No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC8430.2607404658068108No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAACAG8340.2579567597661687No Hit
GTACCGAACATGGAGAACATCGCATCAGGACTCCTAGGACCCCTGCTCGT8320.25733815842380375No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC8300.2567195570814389No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA8260.255482354396709No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAATTTACAAGGCC8190.25331724969843183No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG8120.25115214500015465No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG7880.2437289288917757No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC7870.24341962822059324No Hit
GTTTGATTTTTTGTACAATATGTTCCTGTGGCAATGTGCCCCAACTCCCA7620.23568711144103185No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAATTGGCGAGAAAGTAA7570.23414060808511955No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG7500.23197550338684234No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA7500.23197550338684234No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC7440.2301196993597476No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC7340.22702669264792305No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT7220.22331508459373356No Hit
GACCAGACCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT7200.22269648325136865No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG7190.2223871825801862No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA6990.21620116915653706No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG6930.21434536512944233No Hit
GGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAATTGGCGAGAAAGTA6730.20815935170579322No Hit
CCCGTGTGTCTTGGCCAAAATTCGCAGTCCCAAATCTCCAGTCACTCACC6690.20692214902106337No Hit
GGCCTATTTACAGGAATTTTTCTAAAACACAGTTTGATTTTTTGTACAAT6640.20537564566515107No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAAC6530.2019733382821441No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA6530.2019733382821441No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGA6510.20135473693977915No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA6490.20073613559741427No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG6490.20073613559741427No Hit
GGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAA6430.19888033157031954No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA6430.19888033157031954No Hit
GTAAAAGCCTGTTTAGCTTGTATACATGCATATAAAGGCATTAAAGCAGG6370.19702452754322475No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA6350.19640592620085986No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6330.19578732485849493No Hit
GTACAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT6310.19516872351613002No Hit
GTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTGTACCGAA6220.19238501747548792No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG6210.19207571680430546No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTAT6200.191766416133123No Hit
GTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATAC6150.1902199127772107No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCTAAACAGGCTTTTACTTT6120.18929201076366337No Hit
GGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCTTTTGGGGTTT6070.18774550740775106No Hit
CCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAA6020.18619900405183878No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC6010.18588970338065636No Hit
GCATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC6000.18558040270947387No Hit
ATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACA5840.1806315919705546No Hit
CTCCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGG5820.18001299062818968No Hit
GTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATT5810.1797036899570072No Hit
GGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTGCATCCT5810.1797036899570072No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG5780.17877578794345983No Hit
ATTTACAGGAATTTTTCTAAAACACAGTTTGATTTTTTGTACAATATGTT5380.1664037610961616No Hit
GTACAATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCC5230.16176425102842473No Hit
ACCCTGTACCGAACATGGAGAACATCGCATCAGGACTCCTAGGACCCCTG5220.16145495035724228No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG5200.16083634901487737No Hit
GCACGGGACCATGCAAAACCTGCACGACTACTGCTCAAGGAACCTCTATG5160.15959914633014755No Hit
CCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT5020.15526893693359314No Hit
ATCTTGGGCTTTCGCAAAATACCTATGGGAGTGGGCCTCAGTCCGTTTCT5000.15465033559122823No Hit
GCGATAACCAGGACAAATTGGAGGACAACAGGTTGGTGAGTGACTGGAGA4930.15248523089295105No Hit
TTGACATACTTTCCAATCAATAGGCCTATTTACAGGAATTTTTCTAAAAC4830.14939222418112647No Hit
GGGGCACACCCGTGTGTCTTGGCCAAAATTCGCAGTCCCAAATCTCCAGT4780.1478457208252142No Hit
AGTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATA4780.1478457208252142No Hit
GCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTATCAAGGTATGTTGC4760.14722711948284928No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGT4760.14722711948284928No Hit
CTGTTTACTTAGAAAGGCCTTGTAAATTGGCGAGAAAGTAAAAGCCTGTT4750.14691781881166682No Hit
CTCACCAACCTGTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTC4740.14660851814048437No Hit
GGTGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTG4700.14537131545575452No Hit
GTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCCGCCTGTAACACG4670.14444341344220715No Hit
GATGGGGATATTCCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGC4560.14104110605920014No Hit
GTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCCGC4540.14042250471683523No Hit
GTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAAAT4530.14011320404565278No Hit
GCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGGCACAGACCAGACC4510.13949460270328787No Hit
AGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTATTT4440.13732949800501068No Hit
GAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTG4430.1370201973338282No Hit
ATGCAGAGGAAGATGATAAAACGCCGCAGACACATCCAGCGATAACCAGG4420.13671089666264577No Hit
GCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGTTGACATACT4420.13671089666264577No Hit
GTTCAGGAACAGTGAGCCCTGCTCAGAATACTGTCTCTGCCATATCGTCA4420.13671089666264577No Hit
TAGCAGCAGGATGCAGAGGAAGATGATAAAACGCCGCAGACACATCCAGC4400.13609229532028083No Hit
GCACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4310.13330858927963873No Hit
GTATGTCAACGAATTGTGGGTCTTTTGGGGTTTGCCGCCCCTTTCACGCA4300.13299928860845628No Hit
AAATAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCTTTTGG4300.13299928860845628No Hit
CCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAAGGGACTCAAGATG4260.13176208592372646No Hit
ATACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAG4260.13176208592372646No Hit
ATGTTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG4250.131452785252544No Hit
GTCCCAAATCTCCAGTCACTCACCAACCTGTTGTCCTCCAATTTGTCCTG4170.12897837988308436No Hit
CTTTCCAATCAATAGGCCTATTTACAGGAATTTTTCTAAAACACAGTTTG4170.12897837988308436No Hit
CTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAA4140.12805047786953697No Hit
GCCTATGGCCAAGCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGGC4140.12805047786953697No Hit
GCCCAAGATGATGGGATGGGAATACAGGTGCAGTTTCCGTCCGTAGGTTT4130.1277411771983545No Hit
GGCTTTTACTTTCTCGCCAATTTACAAGGCCTTTCTAAGTAAACAGTATC4090.1265039745136247No Hit
CAATTCGTTGACATACTTTCCAATCAATAGGCCTATTTACAGGAATTTTT4080.12619467384244223No Hit
GGTTTTGCATGGTCCCGTGCTGGTTGTTGATGATCCTGGAATTAGAGGAC4000.12372026847298259No Hit
GTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTA3940.12186446444588785No Hit
CATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAA3920.12124586310352294No Hit
CTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTG3900.12062726176115801No Hit
CCTCTGCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACT3890.12031796108997557No Hit
GTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTCACAAAAC3880.12000866041879311No Hit
CACTTGGCACAGACCAGACCGTTGCCGAGCAACGGGGTAAAGGTTCAGAT3880.12000866041879311No Hit
GCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCCTATGGCCAAG3860.1193900590764282No Hit
TTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTGTGA3820.11815285639169837No Hit
GTTGACATACTTTCCAATCAATAGGCCTATTTACAGGAATTTTTCTAAAA3800.11753425504933346No Hit
GGGTATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTA3790.117224954378151No Hit
GACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGACAGTG3780.11691565370696853No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA3760.11629705236460364No Hit
CATCCAGCGATAACCAGGACAAATTGGAGGACAACAGGTTGGTGAGTGAC3720.1150598496798738No Hit
CTTTATATGCATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAATT3680.11382264699514397No Hit
GACGGAAACTGCACCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAA3680.11382264699514397No Hit
CTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCCGCCTGTAACA3670.11351334632396153No Hit
GGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAACATACCTTGAT3630.11227614363923169No Hit
GCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAGCCAAGAGAAA3610.11165754229686678No Hit
CAATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCC3610.11165754229686678No Hit
GGTATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAA3590.11103894095450188No Hit
ATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTGTG3580.11072964028331941No Hit
GTTTAGCTTGTATACATGCATATAAAGGCATTAAAGCAGGATATCCACAT3570.11042033961213696No Hit
GACACATCCAGCGATAACCAGGACAAATTGGAGGACAACAGGTTGGTGAG3560.11011103894095449No Hit
TACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAG3550.10980173826977205No Hit
ATATTGTACAAAAAATCAAACTGTGTTTTAGAAAAATTCCTGTAAATAGG3500.10825523491385977No Hit
ATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCATG3440.10639943088676501No Hit
TTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCATGG3430.10609013021558257No Hit
GGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACCAGCAC3410.10547152887321765No Hit
CCACTGAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGACTGAGG3400.1051622282020352No Hit
CTGATGGCCTATGGCCAAGCCCCAACCAGTGGGGGTTGCGTCAGCAAACA3390.10485292753085274No Hit
GGATATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACA3370.10423432618848782No Hit
CTTTCACGCAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA3360.10392502551730537No Hit
GTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAAGTTAAGGGAA3350.10361572484612293No Hit
AGGCATAGCAGCAGGATGCAGAGGAAGATGATAAAACGCCGCAGACACAT3330.102997123503758No Hit
CCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTGC3330.102997123503758No Hit
AAATCAAACTGTGTTTTAGAAAAATTCCTGTAAATAGGCCTATTGATTGG3310.10237852216139309No Hit
CCTGTAAATAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT3300.10206922149021064No Hit
GGGGAACACCCGTGTGTCTTGGCCAAAATTCGCAGTCCCAAATCTCCAGT3240.10021341746311589No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)