FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323310
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG18080.5592156134978813No Hit
GTTTACTTAGAAAGGCCTTGTAAATTGGCGAGAAAGTAAAAGCCTGTTTA16820.5202437289288917No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT15110.46735331415669173No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC14050.4345674430113513No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA13700.4237419195199653No Hit
GGAACATATTGTACAAAAAATCAAACTGTGTTTTAGAAAAATTCCTGTAA12360.3822956295815162No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA11840.36621199468002846No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAATTGGCGA11770.3640468899817513No Hit
ACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACAAAACC11580.3581701772292846No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT11570.3578608765581021No Hit
GTCCCGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC11130.3442516470260741No Hit
GTCTCAGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTGTACCGAA10870.3362098295753302No Hit
CTAAAACACAGTTTGATTTTTTGTACAATATGTTCCTGTGGCAATGTGCC10560.326621508768674No Hit
GAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCAC10020.3099192725248214No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG9820.3037332591011722No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9810.30342395842998976No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT9080.28084500943367047No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC8960.277133401379481No Hit
CAATAGGCCTATTTACAGGAATTTTTCTAAAACACAGTTTGATTTTTTGT8890.27496829668120376No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC8880.27465899601002136No Hit
GTTTGATTTTTTGTACAATATGTTCCTGTGGCAATGTGCCCCAACTCCCA8510.26321487117627046No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA8460.2616683678203582No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG8450.2613590671491757No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAATTGGCGAGAAAGTAA8450.2613590671491757No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAACAG8200.25362655036961435No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT8150.252080047013702No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC8080.24991494231542483No Hit
GAGCAGTAGTCGTGCAGGTTTTGCATGGTCCCGTGCTGGTTGTTGATGAT7890.24403822956295818No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA7680.23754291546812656No Hit
GTACCGAACATGGAGAACATCGCATCAGGACTCCTAGGACCCCTGCTCGT7650.2366150134545792No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG7630.23599641211221428No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC7540.23321270607157218No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG7420.22950109801738272No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG7340.22702669264792305No Hit
GACCAGACCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT7240.2239336859360985No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAATTTACAAGGCC7150.22114997989545634No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG7070.21867557452599673No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG7030.2174383718412669No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT7010.216819770498902No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG6970.21558256781417218No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGA6910.2137267637870774No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC6840.21156165908880026No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA6780.20970585506170547No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA6770.20939655439052304No Hit
GGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAATTGGCGAGAAAGTA6590.20382914230923882No Hit
CTCCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGG6490.20073613559741427No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAAC6490.20073613559741427No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA6490.20073613559741427No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA6400.1979524295567721No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6320.1954780241873125No Hit
GGCCTATTTACAGGAATTTTTCTAAAACACAGTTTGATTTTTTGTACAAT6300.1948594228449476No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6280.19424082150258265No Hit
GTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATT6220.19238501747548792No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTAT6200.191766416133123No Hit
GTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTGTACCGAA6180.1911478147907581No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA6130.18960131143484582No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG6100.18867340942129843No Hit
CCCGTGTGTCTTGGCCAAAATTCGCAGTCCCAAATCTCCAGTCACTCACC5950.1840338993535616No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG5910.18279669666883178No Hit
GCATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC5850.18094089264173704No Hit
GTACAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT5850.18094089264173704No Hit
GTAAAAGCCTGTTTAGCTTGTATACATGCATATAAAGGCATTAAAGCAGG5830.1803222912993721No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG5830.1803222912993721No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC5780.17877578794345983No Hit
GGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTGCATCCT5780.17877578794345983No Hit
GTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATAC5770.17846648727227737No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA5720.1769199839163651No Hit
GTACAATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCC5590.17289907519099315No Hit
ATTTACAGGAATTTTTCTAAAACACAGTTTGATTTTTTGTACAATATGTT5580.17258977451981072No Hit
GGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAA5520.17073397049271596No Hit
GGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCTTTTGGGGTTT5420.1676409637808914No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGT5330.1648572577402493No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG5300.16392935572670192No Hit
CCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAA5230.16176425102842473No Hit
GCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGGCACAGACCAGACC4940.15279453156413347No Hit
ATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACA4900.15155732887940368No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCTAAACAGGCTTTTACTTT4890.1512480282082212No Hit
GCACGGGACCATGCAAAACCTGCACGACTACTGCTCAAGGAACCTCTATG4820.149082923509944No Hit
ACCCTGTACCGAACATGGAGAACATCGCATCAGGACTCCTAGGACCCCTG4810.14877362283876155No Hit
GGGGCACACCCGTGTGTCTTGGCCAAAATTCGCAGTCCCAAATCTCCAGT4730.1462992174693019No Hit
GTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAAAT4720.14598991679811946No Hit
ATCTTGGGCTTTCGCAAAATACCTATGGGAGTGGGCCTCAGTCCGTTTCT4720.14598991679811946No Hit
GTCCCAAATCTCCAGTCACTCACCAACCTGTTGTCCTCCAATTTGTCCTG4710.145680616126937No Hit
GAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTG4640.14351551142865981No Hit
TTGACATACTTTCCAATCAATAGGCCTATTTACAGGAATTTTTCTAAAAC4630.14320621075747736No Hit
AAATAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCTTTTGG4630.14320621075747736No Hit
CTGTTTACTTAGAAAGGCCTTGTAAATTGGCGAGAAAGTAAAAGCCTGTT4570.1413504067303826No Hit
GCGATAACCAGGACAAATTGGAGGACAACAGGTTGGTGAGTGACTGGAGA4570.1413504067303826No Hit
GCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTATCAAGGTATGTTGC4560.14104110605920014No Hit
ATGTTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG4510.13949460270328787No Hit
GTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCCGC4440.13732949800501068No Hit
CCTCTGCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACT4390.1357829946490984No Hit
GTATGTCAACGAATTGTGGGTCTTTTGGGGTTTGCCGCCCCTTTCACGCA4350.13454579196436856No Hit
GTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCCGCCTGTAACACG4340.13423649129318613No Hit
CTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTG4300.13299928860845628No Hit
CCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAAGGGACTCAAGATG4260.13176208592372646No Hit
AGTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATA4210.13021558256781418No Hit
GCCTATGGCCAAGCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGGC4210.13021558256781418No Hit
GATGGGGATATTCCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGC4200.12990628189663173No Hit
GCCCAAGATGATGGGATGGGAATACAGGTGCAGTTTCCGTCCGTAGGTTT4200.12990628189663173No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA4170.12897837988308436No Hit
GGTGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTG4110.1271225758559896No Hit
GACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGACAGTG4110.1271225758559896No Hit
CTTTCCAATCAATAGGCCTATTTACAGGAATTTTTCTAAAACACAGTTTG4080.12619467384244223No Hit
CTCACCAACCTGTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTC4070.12588537317125978No Hit
GCACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4070.12588537317125978No Hit
GCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAGCCAAGAGAAA4050.12526677182889487No Hit
CCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT4040.12495747115771241No Hit
GTTCAGGAACAGTGAGCCCTGCTCAGAATACTGTCTCTGCCATATCGTCA4030.12464817048652996No Hit
GTTTAGCTTGTATACATGCATATAAAGGCATTAAAGCAGGATATCCACAT4010.12402956914416505No Hit
ATGCAGAGGAAGATGATAAAACGCCGCAGACACATCCAGCGATAACCAGG3990.12341096780180012No Hit
CTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCCGCCTGTAACA3990.12341096780180012No Hit
ATACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAG3960.12248306578825277No Hit
CATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAA3900.12062726176115801No Hit
TAGCAGCAGGATGCAGAGGAAGATGATAAAACGCCGCAGACACATCCAGC3870.11969935974761065No Hit
CACTTGGCACAGACCAGACCGTTGCCGAGCAACGGGGTAAAGGTTCAGAT3870.11969935974761065No Hit
CAATTCGTTGACATACTTTCCAATCAATAGGCCTATTTACAGGAATTTTT3810.11784355572051593No Hit
GGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAACATACCTTGAT3810.11784355572051593No Hit
AGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTATTT3790.117224954378151No Hit
GCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGTTGACATACT3780.11691565370696853No Hit
GGTTTTGCATGGTCCCGTGCTGGTTGTTGATGATCCTGGAATTAGAGGAC3770.11660635303578609No Hit
GGGTATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTA3740.11567845102223873No Hit
CTTTATATGCATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAATT3730.11536915035105626No Hit
GTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTA3690.11413194766632645No Hit
ATATTGTACAAAAAATCAAACTGTGTTTTAGAAAAATTCCTGTAAATAGG3590.11103894095450188No Hit
GGCTTTTACTTTCTCGCCAATTTACAAGGCCTTTCTAAGTAAACAGTATC3590.11103894095450188No Hit
CATCCAGCGATAACCAGGACAAATTGGAGGACAACAGGTTGGTGAGTGAC3590.11103894095450188No Hit
CTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAA3570.11042033961213696No Hit
CCTGTAAATAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT3500.10825523491385977No Hit
CAATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCC3480.10763663357149485No Hit
CCACTGAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGACTGAGG3480.10763663357149485No Hit
CCCCAATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAG3470.1073273329003124No Hit
GTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAAGTTAAGGGAA3460.10701803222912994No Hit
GGGGAACACCCGTGTGTCTTGGCCAAAATTCGCAGTCCCAAATCTCCAGT3440.10639943088676501No Hit
GGATATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACA3400.1051622282020352No Hit
GTTCAGGAACAGTGAGCCCTGCTCAGAATACTGTCTCAGCCATATCGTCA3380.1045436268596703No Hit
GCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCCTATGGCCAAG3330.102997123503758No Hit
TTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTGTGA3310.10237852216139309No Hit
GTTGACATACTTTCCAATCAATAGGCCTATTTACAGGAATTTTTCTAAAA3310.10237852216139309No Hit
CCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTGC3310.10237852216139309No Hit
GTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTCACAAAAC3290.10175992081902817No Hit
GGGAACACCCGTGTGTCTTGGCCAAAATTCGCAGTCCCAAATCTCCAGTC3260.10083201880548082No Hit
TACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAG3250.10052271813429835No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)