FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488058

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488058
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences532353
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT28500.5353590568664026No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT23990.4506408341833332No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23360.43880658134733913No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA22270.41833144548823803No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA21850.41044194359757524No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCAGCTAGG21680.407248573784688No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT14640.275005494474531No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT14510.2725635057940877No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA13880.2607292529580936No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA12720.23893920011721545No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA12440.23367953219010695No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT12200.22917124539544248No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA11740.22053036237233564No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC11560.21714914727633733No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA11490.2158342302945602No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG11460.21527069444522715No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC11090.20832041897011944No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC11060.20775688312078638No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10800.20287290575989994No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT10750.2019336793443448No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT10100.18972373594212863No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT9970.18728174726168537No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG9820.1844640680150201No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA9690.18202207933457687No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA9330.17525964914258021No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTGAAA9280.1743204227270251No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT9260.17394473216080306No Hit
GATTAACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGC9150.17187843404658187No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTA8670.16286186045725298No Hit
CAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCAGCTAGGTTTTAT8590.16135909819236482No Hit
GTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGG8550.16060771705992077No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA8550.16060771705992077No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC8530.16023202649369872No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC8410.1579778830963665No Hit
CTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATAT8360.1570386566808114No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA8280.15553589441592328No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT8270.15534804913281225No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA8250.1549723585665902No Hit
CTTTATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCTGGC8060.15140329818748086No Hit
CCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCAGCTAGGT7880.14802208309148254No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT7670.14407733214615115No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC7580.14238672459815196No Hit
ATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAACCGTAT7420.13938120006837568No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA7410.13919335478526468No Hit
GGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGA7280.13675136610482144No Hit
AATGAAAACAGGAGACTAAAATTAATTATGCCAGCTAGGTTTTATCCCAA7110.13355799629193413No Hit
TGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGA7040.13224307931015697No Hit
TATCCACATTGTGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG6990.1313038528946019No Hit
ATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCTGGCATAA6990.1313038528946019No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT6880.12923755478038068No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG6850.12867401893104763No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG6840.12848617364793663No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT6780.1273591019492705No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT6740.12660772081682642No Hit
AAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGATAATA6700.12585633968438234No Hit
GAAAACAGGAGACTAAAATTAATTATGCCAGCTAGGTTTTATCCCAATGT6700.12585633968438234No Hit
GTAAATGAAAACAGGAGACTAAAATTAATTATGCCAGCTAGGTTTTATCC6600.12397788685327216No Hit
GTGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGTTGACATACT6580.12360219628705013No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG6520.12247512458838403No Hit
GCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAG6510.12228727930527301No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6170.11590053967949838No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT6160.11571269439638734No Hit
GTACAAGAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG6140.11533700383016532No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA6090.11439777741461023No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG6080.11420993213149921No Hit
GTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTGAG5950.11176794345105598No Hit
AATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATTGGGA5900.11082871703550089No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA5890.11064087175238987No Hit
CGTGTGGACTCTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAA5840.10970164533683478No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG5830.10951380005372374No Hit
TAGATAAAGGGATCAAACCGTATTATCCAGAGTATGTAGTTAATCATTAC5800.1089502642043907No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC5780.10857457363816866No Hit
CACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTTGC5780.10857457363816866No Hit
GGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA5770.10838672835505764No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5740.10782319250572459No Hit
TTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTCCTAAAAC5700.1070718113732805No Hit
TTGTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAAC5680.10669612080705848No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT5670.10650827552394747No Hit
GTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATAC5650.10613258495772541No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGTGTGAAAGGGGCGGCA5590.10500551325905931No Hit
CTTCAAAGACTGTGTGTTTAATGAGTGGGAGGAGTTGGGGGAGGAGGTTA5580.1048176679759483No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT5580.1048176679759483No Hit
GGATATCCACATTGTGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT5560.10444197740972626No Hit
CTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT5530.10387844156039319No Hit
GTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTC5520.10369059627728218No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT5450.10237567929550506No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG5450.10237567929550506No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG5450.10237567929550506No Hit
TCCTCAGGCCATGCAGTGGAACACCACCACTTTCCACCAAACTCTTCAAG5420.101812143446172No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC5380.10106076231372792No Hit
CTTTCACACAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA5380.10106076231372792No Hit
AAATGAAAACAGGAGACTAAAATTAATTATGCCAGCTAGGTTTTATCCCA5370.1008729170306169No Hit
GGACATTGTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATG5350.10049722646439486No Hit
GCCCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCAGCTA5340.10030938118128385No Hit
GACTAAAATTAATTATGCCAGCTAGGTTTTATCCCAATGTTACTAAATAT5340.10030938118128385No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)