FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488059

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488059
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences532353
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT27690.52014358893441No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT23370.4389944266304501No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23140.4346739851188967No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCAGCTAGG21370.4014253700082464No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA21070.3957900115149159No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA20460.38433144924514373No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT15710.29510493976741No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT14830.27857455485364035No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA14490.2721878152278657No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT13020.24457455861054603No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA11880.22316019633588988No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT11770.2210938982216687No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA11720.22015467180611362No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC11310.21245301519856186No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA10960.2058784302896762No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA10620.19949169066390157No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG10460.1964861661341253No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10450.19629832085101429No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC10220.19197787933946084No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG9780.18371268688257603No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT9680.18183423405146584No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC9600.1803314717865777No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA9250.17375688687769206No Hit
GTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGG9080.17056351706480474No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA9020.16943644536613864No Hit
GATTAACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGC9020.16943644536613864No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC8890.1669944566856954No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT8810.16549169442080724No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT8780.1649281585714742No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTA8760.16455246800525214No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA8700.16342539630658603No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC8670.16286186045725298No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTGAAA8490.15948064536125467No Hit
CTTTATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCTGGC8420.1581657283794775No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA8290.1557237396990343No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA8290.1557237396990343No Hit
CCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCAGCTAGGT8190.1538452868679241No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT8110.15234252460303596No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC8100.15215467931992493No Hit
CAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCAGCTAGGTTTTAT7860.1476463925252605No Hit
CTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATAT7800.14651932082659438No Hit
ATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCTGGCATAA7490.1406961170501528No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA7470.14032042648393078No Hit
GGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGA7160.1344972227074892No Hit
AATGAAAACAGGAGACTAAAATTAATTATGCCAGCTAGGTTTTATCCCAA6800.12773479251549255No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA6640.12472926798571624No Hit
AAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGATAATA6610.1241657321363832No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT6500.122099434022162No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG6450.1211602076066069No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG6390.12003313590794079No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG6380.11984529062482975No Hit
TATCCACATTGTGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG6360.11946960005860773No Hit
TGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGA6330.11890606420927466No Hit
ATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAACCGTAT6310.11853037364305263No Hit
GAAAACAGGAGACTAAAATTAATTATGCCAGCTAGGTTTTATCCCAATGT6290.11815468307683061No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT6270.11777899251060855No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA6070.1140220868483882No Hit
GTGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGTTGACATACT6070.1140220868483882No Hit
GGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA6070.1140220868483882No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT6060.11383424156527719No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG6010.11289501514972208No Hit
GTAAATGAAAACAGGAGACTAAAATTAATTATGCCAGCTAGGTTTTATCC5990.11251932458350004No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT5980.11233147930038903No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5980.11233147930038903No Hit
GCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAG5940.11158009816794494No Hit
TAGATAAAGGGATCAAACCGTATTATCCAGAGTATGTAGTTAATCATTAC5900.11082871703550089No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG5860.11007733590305681No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT5850.1098894906199458No Hit
CTCTTGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCCC5820.10932595477061273No Hit
GCCCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCAGCTA5810.10913810948750172No Hit
CTTCAAAGACTGTGTGTTTAATGAGTGGGAGGAGTTGGGGGAGGAGGTTA5770.10838672835505764No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC5710.10725965665639152No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG5670.10650827552394747No Hit
AAATGAAAACAGGAGACTAAAATTAATTATGCCAGCTAGGTTTTATCCCA5670.10650827552394747No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT5660.10632043024083643No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC5650.10613258495772541No Hit
GTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATT5580.1048176679759483No Hit
GTACAAGAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG5540.10406628684350422No Hit
TCCTCAGGCCATGCAGTGGAACACCACCACTTTCCACCAAACTCTTCAAG5510.10350275099417117No Hit
TTGTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAAC5470.10275136986172709No Hit
CTTCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTA5460.10256352457861607No Hit
GTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG5410.10162429816306098No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT5400.10143645287994997No Hit
CTTTCACACAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA5380.10106076231372792No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)