FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002488061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114564
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA10510.9173911525435565No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9570.8353409447994133No Hit
CTTCTGGACTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC9050.789951468174994No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGCCCCACAATTCGTTGACATACT8290.7236130023393038No Hit
GCTTAGATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACAT7130.6223595544848294No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCTTTGTAGGTTGGCGA6720.5865716979155755No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG6380.5568939631996089No Hit
CTCACCAACCTCTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTC5890.5141231102265983No Hit
CCTCTATTACCAATTTTCTTTTGTCTCTGGGTATACATTTGAACCCTAAT5490.47920812820781394No Hit
GTCCACAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA5320.4643692608498307No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT5260.459132013547013No Hit
CCCTTAACTTCATGGGATATGTAATTGGATGTTGGGGTACTTTACCGCAA5210.45476764079466503No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTCAGCACAATA4870.4250899060786984No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG4690.4093781641702455No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4600.401522293216019No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC4430.38668342585803567No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4310.3762089312524004No Hit
CAATATGTTCTTGCGGTAAAGTACCCCAACATCCAATTACATATCCCATG4220.36835306029817394No Hit
AGTTAAGGGAGTAGCCCCATCGTTTGGTTTTATTAGGGTTCAAATGTATA4180.3648615620962955No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC3950.34478544743549455No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT3890.33954820013267695No Hit
GGTTCAGATATTGTTTACACAGAAAGGCTTTGTAGGTTGGCGAGAAAGTG3880.3386753255822073No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA3800.3316923291784505No Hit
GAACATGGAGAACACAGCATCAGGATTCCTAGGACCCCTGCTCGTGTTAC3770.3290737055270416No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3710.323836458224224No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT3620.31598058726999756No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG3460.30201459446248385No Hit
GGGTATACATTTGAACCCTAATAAAACCAAACGATGGGGCTACTCCCTTA3420.2985230962606054No Hit
GACTACTGCCTCACCCATATCGTCAATCTTCTCGAGGACTGGGGACCCTG3380.29503159805872703No Hit
CAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTCAGC3340.29154009985684853No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC3320.28979435075590937No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG3250.28368422890262207No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG3220.2810656052512133No Hit
CATTTGAACCCTAATAAAACCAAACGATGGGGCTACTCCCTTAACTTCAT3210.2801927307007437No Hit
GGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTCAGCACAAT3200.2793198561502741No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC3200.2793198561502741No Hit
GATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGT3100.270591110645578No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCACCCATATCGTCAATCTTCTCG3080.26884536154463884No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC3080.26884536154463884No Hit
GTACCCCAACATCCAATTACATATCCCATGAAGTTAAGGGAGTAGCCCCA3030.26448098879229076No Hit
GTATACATTTGAACCCTAATAAAACCAAACGATGGGGCTACTCCCTTAAC3020.2636081142418212No Hit
CATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA3010.26273523969135154No Hit
GTTCAAATGTATACCCAGAGACAAAAGAAAATTGGTAATAGAGGTAAAAA2940.2566251178380643No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGCCCCACAAT2890.2522607450857163No Hit
ATGTAATTGGATGTTGGGGTACTTTACCGCAAGAACATATTGTGCTGAAA2770.24178625048008098No Hit
CAATTACATATCCCATGAAGTTAAGGGAGTAGCCCCATCGTTTGGTTTTA2760.24091337592961137No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT2740.2391676268286722No Hit
ATATGTTCTTGCGGTAAAGTACCCCAACATCCAATTACATATCCCATGAA2710.23654900317726335No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG2690.23480325407632413No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG2590.2260745085716281No Hit
GCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGCAAAGACCTGACC2590.2260745085716281No Hit
ATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTCAGCACAATATGTT2580.2252016340211585No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA2580.2252016340211585No Hit
GTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTA2570.2243287594706889No Hit
ATGTTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG2570.2243287594706889No Hit
AATGTATACCCAGAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGAC2560.22345588492021928No Hit
AGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAATCT2550.22258301036974965No Hit
GTACAAAACCTTCGGACGGAAATTGCACTTGTATTCCCATCCCATCATCC2530.22083726126881043No Hit
GTTTTATTAGGGTTCAAATGTATACCCAGAGACAAAAGAAAATTGGTAAT2510.2190915121678712No Hit
GTTAAGGGAGTAGCCCCATCGTTTGGTTTTATTAGGGTTCAAATGTATAC2500.21821863761740165No Hit
TGTATACCCAGAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGACTC2480.21647288851646243No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG2460.21472713941552318No Hit
CAGAAAGGCTTTGTAGGTTGGCGAGAAAGTGAAAGCCTGCTTAGATTGTA2440.21298139031458396No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGCCCCACAATTCG2430.21210851576411435No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC2410.2103627666631752No Hit
CTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTG2410.2103627666631752No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA2360.2059983939108271No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC2330.20337977025941834No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG2300.2007611466080095No Hit
GTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTC2240.19552389930519187No Hit
GCCCAAAAGCCCCACAATTCGTTGACATACTTTCCAATCAATAGGTCTAT2230.19465102475472226No Hit
GCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGCCCCACAATTCGTT2230.19465102475472226No Hit
CAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATACAATCTAA2210.19290527565378304No Hit
GTTCAGATATTGTTTACACAGAAAGGCTTTGTAGGTTGGCGAGAAAGTGA2190.19115952655284382No Hit
GCACAGAACATGGAGAACACAGCATCAGGATTCCTAGGACCCCTGCTCGT2180.1902866520023742No Hit
GTAAAGTACCCCAACATCCAATTACATATCCCATGAAGTTAAGGGAGTAG2160.188540902901435No Hit
CACCAACCTCTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTCTG2140.1867951538004958No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTCTGGGTATACATTTGAA2140.1867951538004958No Hit
GGTTAGTGAGTGATTGGAGGTTGGGGACTGCGAATTTTGGCCAGGACACG2110.18417653014908697No Hit
GACCTGACCGTTGCCGGGCAACGGGGTAAAGGTTCAGATATTGTTTACAC2090.18243078104814775No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG2070.18068503194720856No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCAC2070.18068503194720856No Hit
TTGTTTACACAGAAAGGCTTTGTAGGTTGGCGAGAAAGTGAAAGCCTGCT2060.17981215739673895No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG2060.17981215739673895No Hit
GTGAAAGCCTGCTTAGATTGTATACATGCATATAAAGGCATTAAGGCAGG2030.17719353374533012No Hit
TGTTTACACAGAAAGGCTTTGTAGGTTGGCGAGAAAGTGAAAGCCTGCTT2020.1763206591948605No Hit
CTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTG2000.1745749100939213No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA1940.16933766279110365No Hit
CATTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAA1920.16759191369016443No Hit
CTATTACCAATTTTCTTTTGTCTCTGGGTATACATTTGAACCCTAATAAA1860.1623546663873468No Hit
GAATACAAGTGCAATTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA1850.1614817918368772No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT1840.16060891728640758No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAAC1820.1588631681854684No Hit
AACATATTGTGCTGAAAATCAAGCAATGTTTTCGAAAACTGCCTGTAAAT1800.15711741908452917No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG1790.15624454453405956No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1780.15537166998358998No Hit
GACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG1750.15275304633218112No Hit
ATCCAGTGGGGGTTGCGTCAGCAAACACTTGGCAAAGACCTGACCGTTGC1720.15013442268077232No Hit
ACCCTGTTCCGACTACTGCCTCACCCATATCGTCAATCTTCTCGAGGACT1720.15013442268077232No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACCTACAAAGCC1700.14838867357983312No Hit
GGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCC1700.14838867357983312No Hit
GGTACTTTACCGCAAGAACATATTGTGCTGAAAATCAAGCAATGTTTTCG1700.14838867357983312No Hit
ACCCTGCACAGAACATGGAGAACACAGCATCAGGATTCCTAGGACCCCTG1690.14751579902936351No Hit
CCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAG1680.14664292447889388No Hit
TGCTTAGATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACA1670.14577004992842427No Hit
GGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATATTCCTCTTCATCCT1650.14402430082748507No Hit
TATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCAAA1650.14402430082748507No Hit
TACATATCCCATGAAGTTAAGGGAGTAGCCCCATCGTTTGGTTTTATTAG1640.14315142627701546No Hit
GCCTCACCCATATCGTCAATCTTCTCGAGGACTGGGGACCCTGCACAGAA1640.14315142627701546No Hit
CAATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCC1610.14053280262560666No Hit
GGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATT1600.13965992807513705No Hit
ATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTT1600.13965992807513705No Hit
GGCCAATAGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGG1590.13878705352466741No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA1580.13791417897419783No Hit
GGGCTACTCCCTTAACTTCATGGGATATGTAATTGGATGTTGGGGTACTT1570.13704130442372822No Hit
GGATGATGGGATGGGAATACAAGTGCAATTTCCGTCCGAAGGTTTTGTAC1570.13704130442372822No Hit
ATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCAA1560.1361684298732586No Hit
GCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGCAAAGACC1560.1361684298732586No Hit
ATCATATTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT1560.1361684298732586No Hit
GTTTTGTACAGCAACAAGAGGGAAACATAGAGGTTCCTTGAGCAGGAATC1550.135295555322789No Hit
GGTTCCACGCATGCGGCGATGGCCAATAGCCAAGCCCCATCCAGTGGGGG1540.13442268077231942No Hit
TCGTAGGGCTTTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGG1520.1326769316713802No Hit
GCCCAGGATGATGGGATGGGAATACAAGTGCAATTTCCGTCCGAAGGTTT1520.1326769316713802No Hit
GCAGAGGAGCCACAAAGGTTCCACGCATGCGGCGATGGCCAATAGCCAAG1510.1318040571209106No Hit
GTTCCGGAACAGTAAACCCTGTTCCGACTACTGCCTCACCCATATCGTCA1510.1318040571209106No Hit
CTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTGCC1500.13093118257044098No Hit
CCCATATCGTCAATCTTCTCGAGGACTGGGGACCCTGCACAGAACATGGA1500.13093118257044098No Hit
GCACAATATGTTCTTGCGGTAAAGTACCCCAACATCCAATTACATATCCC1500.13093118257044098No Hit
CACGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACCAA1490.13005830801997137No Hit
ATCAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTCA1480.12918543346950176No Hit
GCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACA1480.12918543346950176No Hit
CATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAAGGGGC1460.12743968436856257No Hit
TCCTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTG1460.12743968436856257No Hit
GAGTAGCCCCATCGTTTGGTTTTATTAGGGTTCAAATGTATACCCAGAGA1440.12569393526762335No Hit
ATTCCTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAG1430.12482106071715372No Hit
ATCCTGGGCTTTCGCAAGATTCCTATGGGAGTGGGCCTCAGTCCGTTTCT1390.1213295625152753No Hit
GCTTTGTAGGTTGGCGAGAAAGTGAAAGCCTGCTTAGATTGTATACATGC1390.1213295625152753No Hit
GTCTCTGGGTATACATTTGAACCCTAATAAAACCAAACGATGGGGCTACT1360.11871093886386648No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG1350.11783806431339687No Hit
CCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCA1340.11696518976292726No Hit
CTCCAGTTCCGGAACAGTAAACCCTGTTCCGACTACTGCCTCACCCATAT1330.11609231521245768No Hit
GTAAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCTTTT1320.11521944066198805No Hit
GTTCCGACTACTGCCTCACCCATATCGTCAATCTTCTCGAGGACTGGGGA1320.11521944066198805No Hit
GTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTTTGGCTTTCAGTTATATG1320.11521944066198805No Hit
GTTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCC1300.11347369156104883No Hit
CTCACTAACCTCTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTC1280.11172794246010964No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCTTTTGG1270.11085506790964002No Hit
GGATATGTAATTGGATGTTGGGGTACTTTACCGCAAGAACATATTGTGCT1260.10998219335917041No Hit
CCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTT1240.10823644425823122No Hit
ATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATACAATCTAAGC1230.10736356970776159No Hit
CCACTGAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGACTGAGG1220.10649069515729198No Hit
CCCAGAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGACTCAAGATG1220.10649069515729198No Hit
GTTTTCGAAAACATTGCTTGATTTTCAGCACAATATGTTCTTGCGGTAAA1220.10649069515729198No Hit
GGCTTTGTAGGTTGGCGAGAAAGTGAAAGCCTGCTTAGATTGTATACATG1200.10474494605635279No Hit
GGAGAACACAGCATCAGGATTCCTAGGACCCCTGCTCGTGTTACAGGCGG1200.10474494605635279No Hit
CTTCATGGGATATGTAATTGGATGTTGGGGTACTTTACCGCAAGAACATA1180.10299919695541356No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC1180.10299919695541356No Hit
CACCCATATCGTCAATCTTCTCGAGGACTGGGGACCCTGCACAGAACATG1170.10212632240494397No Hit
CATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTCTCTGGG1170.10212632240494397No Hit
AAGTTAAGGGAGTAGCCCCATCGTTTGGTTTTATTAGGGTTCAAATGTAT1170.10212632240494397No Hit
CCTTTTTACCTCTATTACCAATTTTCTTTTGTCTCTGGGTATACATTTGA1170.10212632240494397No Hit
GACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCTTTTGGGCTTT1160.10125344785447436No Hit
ATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCC1160.10125344785447436No Hit
CAATTCGTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTT1160.10125344785447436No Hit
CCTCTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGT1150.10038057330400475No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)