FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548107
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28670.5230730496052778No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG12790.2333485979927277No Hit
GTGTAAAGGGAGCGGCAAAACCTAAAAGACCCACAATTCTTTGACATACT10820.19740671073348817No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA10150.18518282014278234No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA9040.16493129991041897No Hit
GTGCTGGTGAACGGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8530.15562654737122497No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT8320.15179517867861567No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG8280.15106539416573772No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7850.14322021065229965No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT7730.14103085711366575No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA7540.13756438067749546No Hit
ATTTACAGGAAGTTTCCTAAAACATTGTTTGATTTTTTGTACAATATGTT7450.13592236552352005No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG7260.13245588908734973No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG7240.13209099683091075No Hit
GGTAAGTTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTT6630.12096178300952186No Hit
ATTTGTAGGAAGTTTCCTAAAACATTGTTTGATTTTTTGTACAATATGTT6330.11548839916293717No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT6280.11457616852183972No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG6250.11402883013718124No Hit
CCTGTAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT5760.10508896985442623No Hit
GTATGCAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG5570.10162249341825592No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)