FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488064

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488064
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences531624
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN42800.8050802823047869No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATTTTCAGG30580.5752185755345884No Hit
ATCCTAACCATACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT23810.447872932749462No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG18580.34949513189773224No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG17320.32579417031586233No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGATACTCCCTTAACTTCAT15270.28723308202789943No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG14810.2785803500218199No Hit
ATTTACAGGAAGTTTCCTAAAACATTGTTTGATTTTTTGTACAATATGTT13380.25168163965509455No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCAC12940.24340511338840987No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATA12220.2298617067701985No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA12210.22967360390050112No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT11550.217258814500474No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA11480.21594209441259238No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGGCATACT11420.21481347719440805No Hit
GTTCTATCCTAACCATACCAAATATTTGCCCTTAGATAAAGGCATCAAAC11270.2119919341489474No Hit
GGAACATATTGTACAAAAAATCAAACAATGTTTTAGGAAACTTCCTGTAA11030.20747746527621022No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG10040.18885528117616962No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTAAC9750.1834002979549456No Hit
ATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTATGGTTAG9400.1768166975155373No Hit
AGTCTGTACAACATCTTGAAACCCTTTTTACCGCTATTACCAATTTTCTT9100.1711736114246159No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC9030.16985689133673423No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC8970.16872827411854996No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT8940.1681639655094578No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTC8850.16647103968218138No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG8740.16440190811551023No Hit
TATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAA8570.16120415933065477No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATTTTCA8300.1561253818488255No Hit
ACTTTATTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCT8180.15386814741245694No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAACGTAAGA8110.15255142732457525No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7870.14803695845183815No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7780.14634403262456172No Hit
ATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAA7620.14333438670940366No Hit
CTCATAAGGTGGGAAACTTTACGGGACTTTATTCTTCTACTGTACCTGTC7290.13712699200939008No Hit
GTATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGA7270.13675078626999534No Hit
CTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG7250.13637458053060056No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTATGGTT7220.13581027192150844No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC7200.13543406618211368No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7200.13543406618211368No Hit
ATCTAAGGGCAAATATTTGGTATGGTTAGGATAGAACCTAGCAGGCATGA7040.13242442026695558No Hit
CTATTGATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTG7030.13223631739725822No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACGGGACTTTATTC7000.13167200878816607No Hit
GCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAATAA6850.12885046574270537No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6710.12621702556694206No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAAACCCTTTTTACC6700.12602892269724467No Hit
GGTCTATTTACAGGAAGTTTCCTAAAACATTGTTTGATTTTTTGTACAAT6680.1256527169578499No Hit
CTTGTATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT6680.1256527169578499No Hit
GTTTGGAAGTAATGATTAACTATATTTTCAGGATAATAAGGTTTGATGCC6660.12527651121845515No Hit
TTATTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTT6590.1239597911305735No Hit
CAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAAGGA6470.12170255669420493No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA6450.12132635095481016No Hit
ATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCCATTC6330.11906911651844161No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTGGGGGAGGAGACTA6330.11906911651844161No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT6220.11699998495177041No Hit
TTGGCATACTTTCCAATCAATAGGTCTATTTACAGGAAGTTTCCTAAAAC6120.11511895625479662No Hit
CACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATGTGAG5960.11210931033963856No Hit
CAATAGGTCTATTTACAGGAAGTTTCCTAAAACATTGTTTGATTTTTTGT5950.11192120746994116No Hit
GATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCCATT5860.11022828164266474No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGAAGTTTCCTAAAAC5770.10853535581538831No Hit
CTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCT5750.10815915007599355No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGATACTCCCTTAACTTCAT5710.10740673859720402No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5700.10721863572750667No Hit
CTATATTTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATAT5680.1068424299881119No Hit
CCTTTATCTAAGGGCAAATATTTGGTATGGTTAGGATAGAACCTAGCAGG5650.10627812137901976No Hit
GTACAACATCTTGAAACCCTTTTTACCGCTATTACCAATTTTCTTTTGTC5610.10552570990023025No Hit
AGTTAAGGGAGTATCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA5600.10533760703053285No Hit
GTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTG5590.10514950416083547No Hit
GAATTAGTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACA5440.10232796111537479No Hit
ATTTAAACCCTAATAAAACCAAACGTTGGGGATACTCCCTTAACTTCATG5430.1021398582456774No Hit
ATTGATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCC5410.10176365250628262No Hit
GTCTATTTACAGGAAGTTTCCTAAAACATTGTTTGATTTTTTGTACAATA5380.10119934389719049No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTTTCGCCAACTTACAAGGCC5330.10025882954870359No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACGGGACTTTATTCTTCTACTG5320.10007072667900622No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)