FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences531624
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN42620.801694430650234No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATTTTCAGG32930.6194227499134727No Hit
ATCCTAACCATACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT20330.3824131340947738No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG18760.3528809835522851No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG16750.3150723067431117No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGATACTCCCTTAACTTCAT13940.2622154003581479No Hit
ATTTACAGGAAGTTTCCTAAAACATTGTTTGATTTTTTGTACAATATGTT13920.2618391946187531No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG13620.2561961085278317No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGGCATACT12120.22798067807322467No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCAC12030.22628775224594827No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA11760.221208974764119No Hit
GTTCTATCCTAACCATACCAAATATTTGCCCTTAGATAAAGGCATCAAAC11640.21895174032775044No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATA11380.21406106571561856No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA11050.207853671015605No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG10620.1997652476186177No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT10170.19130061848223556No Hit
GGAACATATTGTACAAAAAATCAAACAATGTTTTAGGAAACTTCCTGTAA10150.1909244127428408No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC10150.1909244127428408No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT9420.17719290325493206No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC9320.17531187455795827No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG9310.17512377168826088No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTAAC9310.17512377168826088No Hit
ATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTATGGTTAG9240.1738070516003792No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTC8930.16797586263976044No Hit
AGTCTGTACAACATCTTGAAACCCTTTTTACCGCTATTACCAATTTTCTT8930.16797586263976044No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATTTTCA8920.16778775977006305No Hit
TATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAA8300.1561253818488255No Hit
GTATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGA8270.15556107323973337No Hit
ATCTAAGGGCAAATATTTGGTATGGTTAGGATAGAACCTAGCAGGCATGA8110.15255142732457525No Hit
GTTTGGAAGTAATGATTAACTATATTTTCAGGATAATAAGGTTTGATGCC8070.15179901584578573No Hit
ACTTTATTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCT7830.14728454697304863No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAACGTAAGA7720.14521541540637742No Hit
CTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG7650.14389869531849578No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7310.13750319774878486No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAAACCCTTTTTACC7310.13750319774878486No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7300.13731509487908747No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC7160.13468165470332413No Hit
CTATTGATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTG7130.134117346094232No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTATGGTT7100.13355303748513989No Hit
ATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAA7000.13167200878816607No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6920.13016718583058703No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACGGGACTTTATTC6630.12471220260936301No Hit
CTCATAAGGTGGGAAACTTTACGGGACTTTATTCTTCTACTGTACCTGTC6630.12471220260936301No Hit
CAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAAGGA6620.12452409973966562No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6490.12207876243359968No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA6390.1201977337366259No Hit
GCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAATAA6380.12000963086692851No Hit
GGTCTATTTACAGGAAGTTTCCTAAAACATTGTTTGATTTTTTGTACAAT6170.11605947060328353No Hit
CTTGTATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT6160.11587136773358614No Hit
ATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCCATTC6150.11568326486388876No Hit
TTATTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTT6110.11493085338509924No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6080.1143665447760071No Hit
CTATATTTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATAT6020.11323792755782282No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5900.11098069312145425No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT5870.11041638451236213No Hit
TTGGCATACTTTCCAATCAATAGGTCTATTTACAGGAAGTTTCCTAAAAC5870.11041638451236213No Hit
CAATAGGTCTATTTACAGGAAGTTTCCTAAAACATTGTTTGATTTTTTGT5810.10928776729417784No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTGGGGGAGGAGACTA5810.10928776729417784No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGATACTCCCTTAACTTCAT5770.10853535581538831No Hit
GAATTAGTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACA5750.10815915007599355No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACGGGACTTTATTCTTCTACTG5710.10740673859720402No Hit
CCTTTATCTAAGGGCAAATATTTGGTATGGTTAGGATAGAACCTAGCAGG5650.10627812137901976No Hit
AGTTAAGGGAGTATCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA5600.10533760703053285No Hit
GTTAAGGGAGTATCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATAC5420.10195175537598002No Hit
ATTTAAACCCTAATAAAACCAAACGTTGGGGATACTCCCTTAACTTCATG5410.10176365250628262No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATACAAGAGAGAAACTACT5390.10138744676688788No Hit
ATGTAATTGGAAGTTGGGGTACCTTGCCACAGGAACATATTGTACAAAAA5350.10063503528809836No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGAAGTTTCCTAAAAC5350.10063503528809836No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)