FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences667302
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN46590.6981846300475647No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA23070.3457205283364953No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA19080.28592751108193887No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGT19000.2847286535931257No Hit
GGAACATATTGTACAAAAAATCAAATCATGTTTTAGAAAACTTCCTGTAA18820.28203122424329613No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC18760.28113208112668625No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAATTTCAT17550.2629993616083872No Hit
GGATAATAGGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACATT17170.2573047885365247No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA15490.2321287812714483No Hit
GTATACATGCATACAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAA15410.23092992378263513No Hit
CAATTACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTTTG15000.22478577915246767No Hit
GTTCAAATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA14940.22388663603585784No Hit
GTGTAAAAGGGGCAGCGAATCCCAAAAGACCCACAATTCGTTGACATACT14450.21654363391687723No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC14290.21414591893925092No Hit
AGTCTGTACAACATCTTGAATCCCTTTATGCCGCTGTTACCAATTTTCTT13880.20800177430908345No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA13440.20140805812061108No Hit
GTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG13420.20110834374840775No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTGTATGCATGTATA13290.1991602003290864No Hit
CTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT13210.1979613428402732No Hit
ACCAAATACTTGCCCTTAGATAAAGGAATCAAACCCTATTATCCAGAGCA13030.1952639134904436No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA12920.19361548444332552No Hit
ATATGTTCCTGTGGCAAGGAGCCCCAACCCCCAATTACATATCCCATGAA12780.19151748383790249No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC12310.18447419609112517No Hit
GTTTACAGGAAGTTTTCTAAAACATGATTTGATTTTTTGTACAATATGTT12160.1822263382996005No Hit
GGGTAAAGGTTCAAATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA12020.18012833769417746No Hit
ATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGTCTGGA11570.17338476431960342No Hit
CTTCAAAGACTGTGTGTTTACTGAATGGGAGGAGTTGGGGGAGGAGATTA11440.17143662090028206No Hit
GTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAATTAATTTTAATC10910.16349419003689483No Hit
CAATAGGCCTGTTTACAGGAAGTTTTCTAAAACATGATTTGATTTTTTGT10770.1613961894314718No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTATGCC10750.16109647505926852No Hit
GGGAATTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGA10590.1586987600816422No Hit
TATCCACATTGTGTAAAAGGGGCAGCGAATCCCAAAAGACCCACAATTCG10550.1580993313372356No Hit
GTTGATAGGTGTAAGCAATTTGTGGGACCCCTAACAATAAATGAAAACAG10300.15435290168469448No Hit
CAATAAATGAAAACAGGAGATTAAAATTAATTATGCCTGCTAGATTTTAT10200.15285432982367803No Hit
CCCTTAGATAAAGGAATCAAACCCTATTATCCAGAGCATGTAGTTAATCA10200.15285432982367803No Hit
ATCTAAGGGCAAGTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAA9800.14686004237961223No Hit
GTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTG9590.14371304147147768No Hit
ATGTAATTGGGGGTTGGGGCTCCTTGCCACAGGAACATATTGTACAAAAA9410.14101561212164807No Hit
ATGTGGATATCCTGCTTTAATGCCTTTGTATGCATGTATACAAGCTAAAC9340.13996661181893655No Hit
TACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTTTGTGAG9310.1395170402606316No Hit
TTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA9040.1354708962358872No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8730.1308253234667362No Hit
GGTGTAAGCAATTTGTGGGACCCCTAACAATAAATGAAAACAGGAGATTA8530.12782817974470329No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA8410.12602989351148355No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA8270.12393189290606052No Hit
ACTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT8050.12063503481182433No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAATCTCCTCC8020.12018546325351939No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT8020.12018546325351939No Hit
TTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTTCTAAAAC7950.11913646295080788No Hit
GTGGATATCCTGCTTTAATGCCTTTGTATGCATGTATACAAGCTAAACAG7950.11913646295080788No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCGAATCCCAAAAGACCCACAAT7820.11718831953148648No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC7810.11703846234538484No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7770.11643903360097826No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC7630.11434103299555523No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC7590.11374160425114865No Hit
GTATGCATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACA7550.11314217550674208No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT7480.11209317520403056No Hit
GAATTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACA7330.10984531741250589No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA7280.10909603148199765No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7250.10864645992369273No Hit
ATGTAAGACCGTTGGCAGGTTCCGGTGGGCGTTCACGGTGGTCTCCATGC7200.10789717399318449No Hit
ATTTGGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA7180.10759745962098122No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA7130.10684817369047298No Hit
GCCTAGCAGCCATGGAAACGATGTATACTTGCGGGAGAGCACTACAGAAT7090.1062487449460664No Hit
ATTACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTTTGTG7080.10609888775996476No Hit
GTCTTACATAAGAGGACTCTTGGACTCTTACATAAGAGGACTCTTGGACT7020.10519974464335488No Hit
GGTTCAAATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA6950.10415074434064336No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT6950.10415074434064336No Hit
GCACCGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG6940.10400088715454173No Hit
GAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCT6940.10400088715454173No Hit
GCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTT6910.10355131559623679No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC6860.10280202966572857No Hit
TGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT6830.10235245810742363No Hit
CCTTTATCTAAGGGCAAGTATTTGGTAACATTGGGATAAAATCTAGCAGG6800.10190288654911869No Hit
GTCTTACATAAGAGGACTCTTGGACTTTCAGCAATGTCAACGACCGACCT6750.10115360061861048No Hit
TTACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTTTGTGA6730.10085388624640718No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6710.10055417187420387No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG6680.10010460031589896No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)