FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences667302
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN46260.6932393429062104No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA21210.3178470917215893No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGT20020.30001408657549355No Hit
GGAACATATTGTACAAAAAATCAAATCATGTTTTAGAAAACTTCCTGTAA18980.28442893922092244No Hit
GGATAATAGGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACATT18750.28098222394058464No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA18140.27184093558838424No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC17310.25940278914194775No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAATTTCAT16300.24426721334568158No Hit
GTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG15670.23482621062127793No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA15270.2288319231772121No Hit
AGTCTGTACAACATCTTGAATCCCTTTATGCCGCTGTTACCAATTTTCTT15130.22673392257178906No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC14950.22403649322195948No Hit
GTTCAAATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA14660.21969063482501178No Hit
GTTTACAGGAAGTTTTCTAAAACATGATTTGATTTTTTGTACAATATGTT14340.2148952048697591No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA13880.20800177430908345No Hit
CAATTACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTTTG13640.20440520184264394No Hit
GTATACATGCATACAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAA13600.20380577309823739No Hit
GTGTAAAAGGGGCAGCGAATCCCAAAAGACCCACAATTCGTTGACATACT13520.20260691560942423No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTGTATGCATGTATA13520.20260691560942423No Hit
GTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAATTAATTTTAATC12700.19031862634908933No Hit
CTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT12580.18852034011586957No Hit
ATATGTTCCTGTGGCAAGGAGCCCCAACCCCCAATTACATATCCCATGAA12500.18732148262705642No Hit
GGGTAAAGGTTCAAATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA11820.17713119397214455No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA11810.17698133678604291No Hit
ATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGTCTGGA11540.17293519276129848No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC11060.16574204782841953No Hit
CTTCAAAGACTGTGTGTTTACTGAATGGGAGGAGTTGGGGGAGGAGATTA10980.16454319033960635No Hit
ACCAAATACTTGCCCTTAGATAAAGGAATCAAACCCTATTATCCAGAGCA10760.16124633224537016No Hit
ATCTAAGGGCAAGTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAA10410.15600133073181258No Hit
CAATAGGCCTGTTTACAGGAAGTTTTCTAAAACATGATTTGATTTTTTGT10280.15405318731249118No Hit
GGGAATTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGA10240.1534537585680846No Hit
ATGTAATTGGGGGTTGGGGCTCCTTGCCACAGGAACATATTGTACAAAAA10020.15015690047384841No Hit
ATGTGGATATCCTGCTTTAATGCCTTTGTATGCATGTATACAAGCTAAAC9880.14805889986842538No Hit
GTTGATAGGTGTAAGCAATTTGTGGGACCCCTAACAATAAATGAAAACAG9660.1447620417741892No Hit
CAATAAATGAAAACAGGAGATTAAAATTAATTATGCCTGCTAGATTTTAT9500.14236432679656286No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTATGCC9340.13996661181893655No Hit
CCCTTAGATAAAGGAATCAAACCCTATTATCCAGAGCATGTAGTTAATCA9280.13906746870232667No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9000.1348714674914806No Hit
GTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTG8980.13457175311927733No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT8930.13382246718876908No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAATCTCCTCC8670.12992618035012632No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA8370.12543046476707698No Hit
GAATTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACA8190.12273303541724737No Hit
TATCCACATTGTGTAAAAGGGGCAGCGAATCCCAAAAGACCCACAATTCG8180.1225831782311457No Hit
GTGGATATCCTGCTTTAATGCCTTTGTATGCATGTATACAAGCTAAACAG8110.12153417792843421No Hit
GGTGTAAGCAATTTGTGGGACCCCTAACAATAAATGAAAACAGGAGATTA8070.1209347491840276No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA7990.11973589169521445No Hit
GTATTTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTATGCCAAG7910.1185370342064013No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT7860.11778774827589308No Hit
GAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCT7730.11583960485657169No Hit
TACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTTTGTGAG7630.11434103299555523No Hit
ATTTGGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA7510.1125427467623355No Hit
TTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA7460.11179346083182726No Hit
TTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTTCTAAAAC7460.11179346083182726No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7340.10999517459860753No Hit
ACTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT7260.10879631710979437No Hit
GTATGCATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACA7210.10804703117928613No Hit
CCTTTATCTAAGGGCAAGTATTTGGTAACATTGGGATAAAATCTAGCAGG7130.10684817369047298No Hit
GGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCTTTTGGGATTC7060.10579917338776146No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC6990.10475017308504994No Hit
GCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTT6960.104300601526745No Hit
ATATTGTACAAAAAATCAAATCATGTTTTAGAAAACTTCCTGTAAACAGG6950.10415074434064336No Hit
GTCTTACATAAGAGGACTCTTGGACTTTCAGCAATGTCAACGACCGACCT6950.10415074434064336No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCGAATCCCAAAAGACCCACAAT6920.10370117278233842No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6830.10235245810742363No Hit
TCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCATT6780.10160317217691539No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG6770.10145331499081375No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6750.10115360061861048No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6680.10010460031589896No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)