FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences596584
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN56400.9453823769997184No Hit
GCCCAAAAGACCCACAATTCTCTGACATACTTTCCAATCAATAGGTCTAT29290.49096187628230054No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT27280.45727005752752337No Hit
CATTTGAACCCTAATAAAACTAAACGTTGGGGATACTCCCTTAACTTCAT25600.42910973140412756No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA23800.3989379534147748No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG23000.3855282743083958No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC22820.3825110965094605No Hit
GTTAAGGGAGTATCCCCAACGTTTAGTTTTATTAGGGTTCAAATGTATAC21240.356026980274362No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA19350.32434661338554166No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC19240.3225027825084146No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC18960.3178093948211819No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTAGTACAATA18140.30406447373714346No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA18140.30406447373714346No Hit
CTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAAC17640.2956834242956566No Hit
CAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTAGT17180.2879728588094887No Hit
AGTTAAGGGAGTATCCCCAACGTTTAGTTTTATTAGGGTTCAAATGTATA17100.28663189089885077No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA16970.2844528180440642No Hit
GGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTAGTACAAT16690.27975943035683154No Hit
GCCCAGAAGACCCACAATTCTCTGACATACTTTCCAATCAATAGGTCTAT16440.2755689056360881No Hit
GTACAATATGTTCTTGCGGTAAAGTACCCCAACTTCCAATTACATATCCC16310.2733898327813015No Hit
GTTTTAGTACAATATGTTCTTGCGGTAAAGTACCCCAACTTCCAATTACA16000.26819358212757965No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA15290.25629249192066833No Hit
ATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTAGTACAATATGTT15260.2557896289541791No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG15240.2554543869765196No Hit
TAATAAAACTAAACGTTGGGGATACTCCCTTAACTTCATGGGATATGTAA15130.25361055609939254No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT14990.2512638622557762No Hit
CTCCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGC14690.2462352325908841No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC14640.24539712764673544No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCGCAAGAACATATTGTACTAAAA14440.24204470787014065No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT13930.23349603743982408No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA13820.23165220656269697No Hit
GTATACATTTGAACCCTAATAAAACTAAACGTTGGGGATACTCCCTTAAC13660.22897027074142118No Hit
GCTTAGATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACAT13650.2288026497525914No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA13600.22796454480844275No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT13560.22729406085312379No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG13500.22628833492014536No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG13420.22494736700950746No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG13130.2200863583334451No Hit
GTACTAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGACCTAT12990.21773966448982873No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC12870.2157282126238719No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC12580.2108672039478095No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACTAAACGTTGGGGATACT12500.20952623603717166No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA12470.2090233730706824No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG11860.19879849275206843No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG11020.1847183296903705No Hit
GGGTATACATTTGAACCCTAATAAAACTAAACGTTGGGGATACTCCCTTA11010.18455070870154078No Hit
ATATTGTACTAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGA10970.18388022474622184No Hit
AACATGGAGAGCACAACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA10840.18170115189143524No Hit
TACATATCCCATGAAGTTAAGGGAGTATCCCCAACGTTTAGTTTTATTAG10680.17901921607015944No Hit
CAATATGTTCTTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATG10640.1783487321148405No Hit
CAATTACATATCCCATGAAGTTAAGGGAGTATCCCCAACGTTTAGTTTTA10640.1783487321148405No Hit
CTAATAAAACTAAACGTTGGGGATACTCCCTTAACTTCATGGGATATGTA10300.17264961849462943No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG10210.17114102959516178No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT10100.16929719871803467No Hit
GGATATGTAATTGGAAGTTGGGGTACTTTACCGCAAGAACATATTGTACT10090.16912957772920492No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC10050.168459093773886No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA9880.16560953696378045No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG9730.1630952221313344No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG9330.15639038257814492No Hit
ATGTAGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC9210.15437893071218806No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC9200.15421130972335834No Hit
ATTCTCTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCG9190.1540436887345286No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG9120.15287034181272044No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG9080.15219985785740148No Hit
ATATGTTCTTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA8880.14884743808080672No Hit
CCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGACTCAAGATG8750.14666836522602014No Hit
GTACAAAACCTTCGGACGGAAACTGCACTTGTATTCCCATCCCATCATCC8710.1459978812707012No Hit
AACATATTGTACTAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAAT8670.14532739731538225No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG8600.1441540503935741No Hit
CAGTAAACCCTGTTCCGACTATTGCCTCACCCATATCGTCAATCTTCTCG8460.14180735654995777No Hit
GATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGT8450.14163973556112802No Hit
ATCATATTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT8430.14130449358346853No Hit
AATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGAC8380.14046638863931987No Hit
GCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGCAGAGACCTGACC8360.14013114666166038No Hit
CATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA8340.1397959046840009No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG8320.13946066270634142No Hit
GGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG8160.13677872688506565No Hit
CCCAAAAGACCCACAATTCTCTGACATACTTTCCAATCAATAGGTCTATT8130.13627586391857643No Hit
CCTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA8030.13459965403027904No Hit
CTTCTGGGCTTTGCTGCCCCTTTTACACAATGTGGCTATCCTGCCTTAAT7950.13325868611964115No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA7830.13124723425368431No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA7780.13040912930953563No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT7780.13040912930953563No Hit
CCCATATCGTCAATCTTCTCGAGGACTGGGGACCCTGCACCGAACATGGA7530.1262186045887922No Hit
CTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTG7480.1253804996446435No Hit
CCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAAT7470.12521287865581376No Hit
AGAACATATTGTACTAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAA7320.12269856382336772No Hit
GGATGATGGGATGGGAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTAC7290.12219570085687849No Hit
CATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGG7230.12118997492390007No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG7190.12051949096858112No Hit
CTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAAGAACATA7190.12051949096858112No Hit
TGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGACTC7190.12051949096858112No Hit
GCCCAGGATGATGGGATGGGAATACAAGTGCAGTTTCCGTCCGAAGGTTT7170.12018424899092166No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCAC7130.11951376503560271No Hit
CTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTGCC7020.1176699341584756No Hit
CTTTTGGGCTTTGCTGCCCCTTTTACACAATGTGGCTATCCTGCCTTAAT7020.1176699341584756No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6990.11716707119198638No Hit
GGTATACATTTGAACCCTAATAAAACTAAACGTTGGGGATACTCCCTTAA6950.11649658723666743No Hit
ATCAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTA6900.11565848229251874No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG6850.11482037734837004No Hit
CCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTT6800.11398227240422137No Hit
ATCCTGGGCTTTCGCAAGATTCCTATGGGAGTGGGCCTCAGTCCGTTTCT6790.11381465141539163No Hit
GTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA6770.11347940943773215No Hit
GTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTC6710.11247368350475373No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6680.11197082053826451No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT6670.11180319954943478No Hit
GCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTACCAAGGTATGTTGC6620.1109650946052861No Hit
GCCTTAATGCCTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTT6620.1109650946052861No Hit
CGTTTAGTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATT6610.11079747361645637No Hit
CACGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACCAA6570.11012698966113742No Hit
AACTAAACGTTGGGGATACTCCCTTAACTTCATGGGATATGTAATTGGAA6430.10778029581752109No Hit
GGATACTCCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTT6400.10727743285103189No Hit
ATGTTCTTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGT6390.10710981186220214No Hit
GAATATGATAAAACGCCGCAGACACATCCAGCGATAGCCAGGACAAATTG6380.1069421908733724No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCT6370.10677456988454266No Hit
CTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACA6340.10627170691805345No Hit
CTTCTGGACTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC6250.10476311801858583No Hit
TTTGAACCCTAATAAAACTAAACGTTGGGGATACTCCCTTAACTTCATGG6220.1042602550520966No Hit
ATTCCTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAG6200.10392501307443713No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT6160.10325452911911819No Hit
ATCCAGTGGGGGTTGCGTCAGCAAACACTTGGCAGAGACCTGACCGTTGC6150.10308690813028844No Hit
CAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATACAATCTAA6120.10258404516379922No Hit
CAAGAACATATTGTACTAAAACTCAAGCAATGTTTTCGAAAACTGCCTGT6100.10224880318613977No Hit
GTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAGTAT6090.10208118219731002No Hit
CCCTAATAAAACTAAACGTTGGGGATACTCCCTTAACTTCATGGGATATG6060.10157831923082081No Hit
TCCTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTG6020.10090783527550186No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)