FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences596584
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN56110.940521368323656No Hit
GCCCAAAAGACCCACAATTCTCTGACATACTTTCCAATCAATAGGTCTAT30770.5157697826291017No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG28300.4743673983881566No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT25630.4296125943706168No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA23850.39977605835892344No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC22340.37446528904563314No Hit
CATTTGAACCCTAATAAAACTAAACGTTGGGGATACTCCCTTAACTTCAT22020.36910141740308156No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC21580.3617260938945731No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTAGTACAATA20710.347143067866386No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA19380.3248494763520309No Hit
GTTAAGGGAGTATCCCCAACGTTTAGTTTTATTAGGGTTCAAATGTATAC19320.32384375041905245No Hit
GCCCAGAAGACCCACAATTCTCTGACATACTTTCCAATCAATAGGTCTAT19260.32283802448607407No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC18730.313954112078098No Hit
GTTTTAGTACAATATGTTCTTGCGGTAAAGTACCCCAACTTCCAATTACA17620.29534818231799714No Hit
GGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTAGTACAAT17390.2914928995749132No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA17160.2876376168318292No Hit
GTACAATATGTTCTTGCGGTAAAGTACCCCAACTTCCAATTACATATCCC16930.28378233408874526No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT16900.28327947112225604No Hit
CTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAAC16110.2700374130047068No Hit
CAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTAGT15980.2678583401499202No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA15890.26634975125045257No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT15290.25629249192066833No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA15270.2559572499430089No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG14940.25042575731162753No Hit
AGTTAAGGGAGTATCCCCAACGTTTAGTTTTATTAGGGTTCAAATGTATA14740.24707333753503277No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCGCAAGAACATATTGTACTAAAA14560.24405615973609754No Hit
ATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTAGTACAATATGTT14350.24053611897067304No Hit
CTCCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGC13760.23064648062971851No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACTAAACGTTGGGGATACT13600.22796454480844275No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA13490.22612071393131564No Hit
GTATACATTTGAACCCTAATAAAACTAAACGTTGGGGATACTCCCTTAAC13440.22528260898716695No Hit
TAATAAAACTAAACGTTGGGGATACTCCCTTAACTTCATGGGATATGTAA13310.22310353613238035No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG13260.22226543118823167No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA13250.22209781019940195No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG13240.2219301892105722No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC13210.22142732624408296No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG13190.22109208426642352No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC13100.21958349536695584No Hit
GTACTAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGACCTAT13060.2189130114116369No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT12990.21773966448982873No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA12620.2115376879031285No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC12280.20583857428291744No Hit
GCTTAGATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACAT12250.20533571131642817No Hit
ATATTGTACTAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGA11560.19376986308717634No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT11370.19058506429941133No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG10630.17818111112601076No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG10360.17365534442760786No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG10290.17248199750579968No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG9920.1662800209190994No Hit
GGATATGTAATTGGAAGTTGGGGTACTTTACCGCAAGAACATATTGTACT9900.16594477894143994No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA9890.1657771579526102No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT9740.16326284312016415No Hit
ATTCTCTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCG9740.16326284312016415No Hit
GGGTATACATTTGAACCCTAATAAAACTAAACGTTGGGGATACTCCCTTA9670.16208949619835597No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG9510.15940756037708018No Hit
ATGTAGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC9430.15806659246644228No Hit
CAATATGTTCTTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATG9360.1568932455446341No Hit
AACATGGAGAGCACAACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA9270.15538465664516649No Hit
TACATATCCCATGAAGTTAAGGGAGTATCCCCAACGTTTAGTTTTATTAG9210.15437893071218806No Hit
CAATTACATATCCCATGAAGTTAAGGGAGTATCCCCAACGTTTAGTTTTA9060.15186461587974198No Hit
CTTCTGGGCTTTGCTGCCCCTTTTACACAATGTGGCTATCCTGCCTTAAT9010.15102651093559333No Hit
GTACAAAACCTTCGGACGGAAACTGCACTTGTATTCCCATCCCATCATCC8880.14884743808080672No Hit
ATATGTTCTTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA8850.1483445751143175No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC8820.1478417121478283No Hit
CTAATAAAACTAAACGTTGGGGATACTCCCTTAACTTCATGGGATATGTA8770.1470036072036796No Hit
CTTTTGGGCTTTGCTGCCCCTTTTACACAATGTGGCTATCCTGCCTTAAT8750.14666836522602014No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC8720.14616550225953093No Hit
ATCATATTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT8620.14448929237123354No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG8550.14331594544942539No Hit
GGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG8470.1419749775387875No Hit
CTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTG8230.1379520738068738No Hit
CCCAAAAGACCCACAATTCTCTGACATACTTTCCAATCAATAGGTCTATT8090.1356053799632575No Hit
GCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGCAGAGACCTGACC8060.13510251699676828No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA8040.1347672750191088No Hit
CAGTAAACCCTGTTCCGACTATTGCCTCACCCATATCGTCAATCTTCTCG7990.1339291700749601No Hit
CCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGACTCAAGATG7980.1337615490861304No Hit
CTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTGCC7810.13091199227602482No Hit
CATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA7630.12789481447708956No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCAC7610.1275595724994301No Hit
GTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTC7560.1267214675552814No Hit
AACATATTGTACTAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAAT7540.12638622557762194No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7540.12638622557762194No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG7520.12605098359996245No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG7380.12370428975634613No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC7370.12353666876751639No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG7360.12336904777868665No Hit
CCTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA7270.12186045887921902No Hit
GATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGT7220.12102235393507033No Hit
AATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGAC7190.12051949096858112No Hit
CCCATATCGTCAATCTTCTCGAGGACTGGGGACCCTGCACCGAACATGGA7060.11834041811379455No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6990.11716707119198638No Hit
CATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGG6980.11699945020315665No Hit
GTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA6940.11632896624783769No Hit
GGATGATGGGATGGGAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTAC6840.11465275635954032No Hit
CTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAAGAACATA6700.11230606251592398No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT6610.11079747361645637No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA6570.11012698966113742No Hit
CTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACA6490.1087860217504995No Hit
GTTTTCGAAAACATTGCTTGAGTTTTAGTACAATATGTTCTTGCGGTAAA6450.10811553779518056No Hit
ATCCTGGGCTTTCGCAAGATTCCTATGGGAGTGGGCCTCAGTCCGTTTCT6390.10710981186220214No Hit
ATCAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTA6300.1056012229627345No Hit
ATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATACAATCTAAGC6240.10459549702975608No Hit
AGAACATATTGTACTAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAA6220.1042602550520966No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG6180.10358977109677764No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCT6140.10291928714145872No Hit
GCCTTAATGCCTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTT6090.10208118219731002No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT6040.10124307725316133No Hit
ATTCCTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAG6020.10090783527550186No Hit
GTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAGTAT6000.10057259329784238No Hit
AACTAAACGTTGGGGATACTCCCTTAACTTCATGGGATATGTAATTGGAA6000.10057259329784238No Hit
GTTTTATCATATTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTG5980.10023735132018291No Hit
GCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTACCAAGGTATGTTGC5970.10006973033135316No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)