FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences542285
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACCGCATGTTCAGG33660.6207068239025605No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAC31740.5853010870667638No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTGAAATTGATTATGCCTGCTAGG28380.5233410476041196No Hit
GGATATTATCAACAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG24220.44662861779322677No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20340.37507952460422106No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTCAATCTCCTTTTTTCA17980.3315599730768876No Hit
ATTATCAACAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAA16410.3026084070184497No Hit
TCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACCGCA15510.28601196787667005No Hit
CTCTAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA15160.2795577970993112No Hit
GAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACCGCATGTTCA14540.26812469457941857No Hit
CTCCAAAAGACACCAAATACTCTAGAACAGTTTCTCTTCCAAAAGTAAGA14410.26572743114782815No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC14140.26074849940529427No Hit
GTTCAGGATAGTAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA14130.2605640945259412No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC14080.2596420701291756No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTT13760.25374111398987614No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTGTTGATAATATCCTCTTTC13190.24323003586674904No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC12960.23898872364162757No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC12960.23898872364162757No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA11950.22036383082696367No Hit
GTGTAAAAGGGGCAGCAAAGCCCAGAAGACCCACAATTCTTTGACATACT11780.21722894787796085No Hit
GTAGTAAGCTATGTCAATGTTAACATGGGCCTAAAAATCAGACAACTACT11670.21520049420507667No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCTCTATTACCAATTTTCTT11420.21059037222124896No Hit
GGAACATATTGTACAAAAATTGAAACAATGTTTTCGGAAACTTCCTATAA11230.2070866795135399No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG11140.20542703559936196No Hit
GGATAGTAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT10660.19657560139041277No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10330.19049024037176024No Hit
GGATAGAACCTAGCAGGCATAATCAATTTCAATCTCCTTTTTTCATTAAC10290.18975262085434783No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGT10140.18698654766405118No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAATATGTT10040.18514249887052012No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT9670.17831951833445514No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT9480.17481582562674608No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC9380.17297177683321502No Hit
ATGTTAACATGGGCCTAAAAATCAGACAACTACTGTGGTTTCACATTTCC9100.167808440211328No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA9010.16614879629715001No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTGCCAATTACATATCCCATGAA8940.16485796214167828No Hit
CTTTTACACAATGTGGTTACCCAGCATTAATGCCTTTATATGCATGTATT8880.16375153286555963No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC8790.16209188895138166No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8770.16172307919267542No Hit
GTTGATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCC8720.16080105479590992No Hit
CCCTTAACTTCATGGGATATGTAATTGGCAGTTGGGGTACCTTGCCACAG8590.15840379136431948No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC8520.15711295720884774No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA8480.15637533769143533No Hit
CTTACAGTTAATGAAAAAAGGAGATTGAAATTGATTATGCCTGCTAGGTT8270.15250283522502003No Hit
ATCCTGAACATGCGGTTAATCATTACTTCCAAACTAGACATTATTTACAT8020.14789271324119235No Hit
ATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTTGGA8010.14770830836183926No Hit
GAATTAGTAGTAAGCTATGTCAATGTTAACATGGGCCTAAAAATCAGACA7920.1460486644476613No Hit
ATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACCGCATGTTCAGGAT7890.14549544980960197No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC7740.14272937661930535No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG7700.1419917571018929No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT7660.1412541375844805No Hit
ATCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAA7520.13867246927353696No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7460.13756603999741832No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGCGAGAGGACAACAGAGT7340.13535318144518102No Hit
CATATAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAAGGGGCAGCA7330.13516877656582793No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAATTGATGAATCTGGCCAC7190.13258710825488443No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT7130.13148067897876578No Hit
TTACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTA7040.1298210350645878No Hit
CTTCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACCG7000.12908341554717537No Hit
CAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTGAAATTGAT6970.12853020090911607No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA6920.1276081765123505No Hit
GCCTTCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAAC6900.12723936675364428No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCGTCTATTCG6870.12668615211558498No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAAT6870.12668615211558498No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA6740.12428888868399458No Hit
CCAAAAGACACCAAATACTCTAGAACAGTTTCTCTTCCAAAAGTAAGACA6690.12336686428722904No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCT6620.12207603013175729No Hit
ATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTTGGAAG6570.12115400573499174No Hit
TTACTATCCTGAACATGCGGTTAATCATTACTTCCAAACTAGACATTATT6560.12096960085563864No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTATAGGAAGTTTCCGAAAAC6530.1204163862175793No Hit
ATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCCAGTC6480.11949436182081379No Hit
CTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCT6460.11912555206210755No Hit
GTATCGGGAGGCCTTAGAATCTCCGGAACATTGTTCACCTCACCATACAG6440.11875674230340134No Hit
GTAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTTAGGAT6310.11635947887181095No Hit
CCCTTAGATAAAGGCATTAAACCTTACTATCCTGAACATGCGGTTAATCA6250.1152530495956923No Hit
GCTTACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC6150.11340900080216122No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA6070.11193376176733635No Hit
GTGTAGTTTCTCTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAA6040.11138054712927704No Hit
GCCAATTACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTT6030.11119614224992393No Hit
GTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAAC6010.11082733249121772No Hit
CAATAGGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGT5980.1102741178531584No Hit
ATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTGAAATTGATTA5960.10990530809445218No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACA5910.10898328369768664No Hit
ATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAATTGATGAATCTG5910.10898328369768664No Hit
GTACAACATCTTGAATCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC5830.10750804466286178No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5830.10750804466286178No Hit
CAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTGAAATT5800.10695483002480247No Hit
GAATATGGTGACCCACAAAATGAGGCACTGCGTGTAGTTTCTCTCTTATA5770.10640161538674313No Hit
GTAATTGGCAGTTGGGGTACCTTGCCACAGGAACATATTGTACAAAAATT5730.10566399586933072No Hit
TCACCATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAATTGATGA5650.10418875683450583No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG5580.10289792267903408No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACTGCCGTG5560.10252911292032788No Hit
GATTATGCCTGCTAGGTTCTATCCTAACCTTACCAAATATTTGCCCTTAG5560.10252911292032788No Hit
GCATTAATGCCTTTATATGCATGTATTCAAGCTAAGCAGGCTTTCACTTT5550.10234470804097477No Hit
GTTTGGAAGTAATGATTAACCGCATGTTCAGGATAGTAAGGTTTAATGCC5550.10234470804097477No Hit
ATTTATGCCTACAGCCTCCCAGTACAAAGACCTTTAACCTAATCTCCTCC5540.10216030316162167No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTCTGG5490.10123827876485612No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)