FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences542285
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACCGCATGTTCAGG37590.6931779414883318No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAC30700.5661229796140406No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTGAAATTGATTATGCCTGCTAGG27180.5012124620817466No Hit
GGATATTATCAACAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG22100.40753478337036797No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20310.3745263099661617No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTCAATCTCCTTTTTTCA18760.34594355366643004No Hit
CTCTAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA16950.31256627050351754No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC16410.3026084070184497No Hit
GTTCAGGATAGTAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA16210.29892030943138753No Hit
ATTATCAACAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAA15100.27845136782319263No Hit
GAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACCGCATGTTCA15020.27697612878836775No Hit
TCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACCGCA14480.2670182653032999No Hit
CTCCAAAAGACACCAAATACTCTAGAACAGTTTCTCTTCCAAAAGTAAGA14240.2625925481988253No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC14060.2592732603704694No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTT13110.24175479683192416No Hit
GTAGTAAGCTATGTCAATGTTAACATGGGCCTAAAAATCAGACAACTACT12780.2356694358132716No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC12570.23179693334685636No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA12070.22257668937920097No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAATATGTT11890.21925740155084505No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTGTTGATAATATCCTCTTTC11530.21261882589413317No Hit
GGATAGTAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT11460.21132799173866143No Hit
GGAACATATTGTACAAAAATTGAAACAATGTTTTCGGAAACTTCCTATAA11230.2070866795135399No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCTCTATTACCAATTTTCTT11030.20339858192647778No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC10820.19952607946006254No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGT10790.1989728648220032No Hit
GGATAGAACCTAGCAGGCATAATCAATTTCAATCTCCTTTTTTCATTAAC10580.19510036235558792No Hit
GTGTAAAAGGGGCAGCAAAGCCCAGAAGACCCACAATTCTTTGACATACT10330.19049024037176024No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT9750.17979475736928No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9530.17573785002351164No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9240.1703901085222715No Hit
CTTTTACACAATGTGGTTACCCAGCATTAATGCCTTTATATGCATGTATT9070.16725522557326866No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG9040.16670201093520937No Hit
ATGTTAACATGGGCCTAAAAATCAGACAACTACTGTGGTTTCACATTTCC9030.16651760605585625No Hit
GTTGATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCC8860.16338272310685342No Hit
ATCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAA8780.16190748407202857No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA8670.15987903039914436No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT8500.15674414745014154No Hit
ATCCTGAACATGCGGTTAATCATTACTTCCAAACTAGACATTATTTACAT8490.15655974257078842No Hit
CTTACAGTTAATGAAAAAAGGAGATTGAAATTGATTATGCCTGCTAGGTT8410.15508450353596356No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTGCCAATTACATATCCCATGAA8410.15508450353596356No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA8290.15287164498372627No Hit
CCCTTAACTTCATGGGATATGTAATTGGCAGTTGGGGTACCTTGCCACAG8260.15231843034566694No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC8220.15158081082825453No Hit
ATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTTGGA8190.15102759619019518No Hit
GAATTAGTAGTAAGCTATGTCAATGTTAACATGGGCCTAAAAATCAGACA8130.14992116691407653No Hit
ATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACCGCATGTTCAGGAT8070.14881473763795788No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAAT7900.14567985468895506No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT7680.1416229473431867No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG7510.13848806439418387No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC7430.13701282535935902No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7240.13350913265164996No Hit
TTACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTA6980.12871460578846916No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC6910.12742377163299742No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA6840.12613293747752566No Hit
GTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAAC6840.12613293747752566No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT6780.125026508201407No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCT6760.12465769844270079No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAATTGATGAATCTGGCCAC6560.12096960085563864No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTATAGGAAGTTTCCGAAAAC6530.1204163862175793No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTCTGG6460.11912555206210755No Hit
CTTCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACCG6440.11875674230340134No Hit
TTACTATCCTGAACATGCGGTTAATCATTACTTCCAAACTAGACATTATT6420.11838793254469514No Hit
ATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCCAGTC6270.1156218593543985No Hit
GTTTGGAAGTAATGATTAACCGCATGTTCAGGATAGTAAGGTTTAATGCC6240.1150686447163392No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA6230.11488423983698609No Hit
GTATCGGGAGGCCTTAGAATCTCCGGAACATTGTTCACCTCACCATACAG6160.11359340568151434No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACA6140.11322459592280812No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA6110.1126713812847488No Hit
GTACAACATCTTGAATCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC6070.11193376176733635No Hit
CATATAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAAGGGGCAGCA6040.11138054712927704No Hit
GCCTTCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAAC6030.11119614224992393No Hit
GCTTACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC6020.11101173737057082No Hit
CAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTGAAATTGAT5980.1102741178531584No Hit
ATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTTGGAAG5960.10990530809445218No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCGTCTATTCG5940.10953649833574597No Hit
GTAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTTAGGAT5820.10732363978350867No Hit
GTGTAGTTTCTCTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAA5810.10713923490415557No Hit
CAATAGGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGT5800.10695483002480247No Hit
GCCTTTATCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAG5780.10658602026609623No Hit
CTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCT5780.10658602026609623No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGCGAGAGGACAACAGAGT5760.10621721050739003No Hit
CCAAAAGACACCAAATACTCTAGAACAGTTTCTCTTCCAAAAGTAAGACA5740.10584840074868382No Hit
GATAAAGGCATTAAACCTTACTATCCTGAACATGCGGTTAATCATTACTT5570.10271351779968098No Hit
GCATTAATGCCTTTATATGCATGTATTCAAGCTAAGCAGGCTTTCACTTT5520.10179149340291543No Hit
GTTTGGGACTCTACCGTCCCCTTCTGCGTCTGCCGTTCCGACCGCCCACG5510.10160708852356233No Hit
CCCTTAGATAAAGGCATTAAACCTTACTATCCTGAACATGCGGTTAATCA5490.10123827876485612No Hit
GTTCTAGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCCCGC5480.10105387388550302No Hit
GGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG5470.10086946900614992No Hit
ATTTATGCCTACAGCCTCCCAGTACAAAGACCTTTAACCTAATCTCCTCC5460.10068506412679679No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)