FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences552458
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACTATACCTGTCTTTAATCCTAACTGGAAAACTCCATCTTTTCCTGAT34680.6277400272961926No Hit
TCCATAGGGTATGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTACA23200.41994142541152446No Hit
ACTATACCTGTCTTTAATCCTAACTGGAAAACTCCATCTTTTCCTGATAT21960.3974962802602189No Hit
ATGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTACATTCTCTGGAT20300.3674487472350839No Hit
GTTTTATACCTTTCTCTAGGGGCAAATATTTTGTAGAGATGGGAAAAAAT19810.3585792947156164No Hit
TTCTAAAACAGTCTTTGATTTTCATTCTGATGTGTTCCTGTGGTAATGAC19430.3517009437821518No Hit
ATTTGTAGGTCCTCTAACAGTAAATGAAAAACGAAGATTAAACTTAGTCA15990.2894337669107878No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC15830.286537619149329No Hit
TTATTAACAAATGTGAACAATTTGTAGGTCCTCTAACAGTAAATGAAAAA15750.2850895452685996No Hit
CTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAATCCCAACAAA15270.27640110198422324No Hit
TGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTACATTCTCTGGATA15140.274047981928038No Hit
CTCTTCTACTATACCTGTCTTTAATCCTAACTGGAAAACTCCATCTTTTC14620.2646355017032969No Hit
CTTTAATCCTAACTGGAAAACTCCATCTTTTCCTGATATTCATTTGCACC13970.2528699014223706No Hit
GGATAATAAGGTTTTATACCTTTCTCTAGGGGCAAATATTTTGTAGAGAT13830.25033577213109415No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC13350.24164732884671777No Hit
ACTACATTCTCTGGATAATAAGGTTTTATACCTTTCTCTAGGGGCAAATA13330.2412853103765354No Hit
ATTTTGTAGAGATGGGAAAAAATCTAGCAGGCATGACTAAGTTTAATCTT13170.23838916261507662No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12890.23332090403252373No Hit
CAATTCGTTGACATACTTTCCAATCAATAGGCCGGTTAACAGGGAGTTTT12880.23313989479743255No Hit
CTTCTACTATACCTGTCTTTAATCCTAACTGGAAAACTCCATCTTTTCCT12760.23096778397633846No Hit
CTCTAACAGTAAATGAAAAACGAAGATTAAACTTAGTCATGCCTGCTAGA12610.22825264544997087No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12510.22644255309905909No Hit
CTTTATACCTCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA12160.22010722987086803No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCTCCCGTG12150.21992622063577685No Hit
ATGTAAGACCTTGGGCAATATTTGGTGGGCGTTCACGGTGGTCTCCATGC11360.20562649106357409No Hit
CTATTTACATACCCTATGGAAGGCGGGCATCTTATATAAAAGAGAAACTA11150.2018252971266594No Hit
CATTTAAATCCCAACAAAACAAAAAGATGGGGATATTCCCTACATTTCAT11030.1996531863055653No Hit
GTATGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTACATTCTCTGG10940.19802410318974475No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCGTTGACATACT10850.19639502007392415No Hit
CTGTTAGAGGACCTACAAATTGTTCACATTTGTTAATAATGTCCTGGTGC10590.19168877996155365No Hit
CTACAAAATATTTGCCCCTAGAGAAAGGTATAAAACCTTATTATCCAGAG10280.18607749367372722No Hit
CCCTATGGAAGGCGGGCATCTTATATAAAAGAGAAACTACACGTAGCGCC10040.18173327203153905No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAATATTTGGTGGGCGTTC10010.18119024432626554No Hit
CCTACAAATTGTTCACATTTGTTAATAATGTCCTGGTGCAAATGAATATC9790.1772080411542597No Hit
GCTTTACTCTTCTACTATACCTGTCTTTAATCCTAACTGGAAAACTCCAT9790.1772080411542597No Hit
CCTTTATACCTCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA9690.17539794880334794No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA9610.17394987492261857No Hit
TAACAAATGTGAACAATTTGTAGGTCCTCTAACAGTAAATGAAAAACGAA9280.1679765701646098No Hit
TTCTTGGACGGTCCCTCTCGAATGGGGGAAGAATCATTCCACCACCAATC9220.16689051475406275No Hit
GGACATTATTAACAAATGTGAACAATTTGTAGGTCCTCTAACAGTAAATG9070.16417537622769512No Hit
TGTTAATAATGTCCTGGTGCAAATGAATATCAGGAAAAGATGGAGTTTTC8930.16164124693641868No Hit
GGGTATGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTACATTCTCT8660.15675399758895697No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTCTGTATGCGTGTATT8370.15150472977131293No Hit
CACGCATACAGAGGCATTAAAGCAGGATATCCACATTGTGTAAAAGGGGC8310.15041867436076586No Hit
GCCTAGCAGCCATGGAAAGGATGTATATTTCCGGGAAAGAACGACAGAAT8280.14987564665549236No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT8200.14842757277476298No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT7980.14444536960275714No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCTCTGTTACCAATTTTCTT7640.1382910556096572No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGGAAGGCCTTGTAAGTTGGCGA7570.137023990964019No Hit
GTTAGAGGACCTACAAATTGTTCACATTTGTTAATAATGTCCTGGTGCAA7570.137023990964019No Hit
GTAGGGAATATCCCCATCTTTTTGTTTTGTTGGGATTTAAATGTATACCC7570.137023990964019No Hit
GTTTTAGAAAACTCCCTGTTAACCGGCCTATTGATTGGAAAGTATGTCAA7490.1355759170832896No Hit
GTCCAAGAAAGCCCCATGATGTAGCTCTTGTTCCCAAGAATAAGGTGACC7040.12743050150418675No Hit
ATTCCCTACATTTCATGGGTTATGTAATTGGAAGTTGGGGGTCATTACCA7020.1270684830340044No Hit
TATCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCG6940.125620409153275No Hit
CATTTGCACCAGGACATTATTAACAAATGTGAACAATTTGTAGGTCCTCT6940.125620409153275No Hit
ATGTAGGGAATATCCCCATCTTTTTGTTTTGTTGGGATTTAAATGTATAC6930.12543939991818384No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC6880.12453435374272796No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGATTTCTTTCCTTCAGTAAG6790.12290527062690738No Hit
GCCATGGAAAGGATGTATATTTCCGGGAAAGAACGACAGAATTATCTGTC6660.1205521505707221No Hit
GTATAAAACCTTATTATCCAGAGAATGTAGTTAATCATTACTTCCAAACC6660.1205521505707221No Hit
GGGATATTCCCTACATTTCATGGGTTATGTAATTGGAAGTTGGGGGTCAT6650.12037114133563094No Hit
GTGCAAGGTCCCCAATCCTCGAGAAGATTGACGAGATGAGTGAGGCAGTA6640.12019013210053976No Hit
TTTCATGGGTTATGTAATTGGAAGTTGGGGGTCATTACCACAGGAACACA6580.11910407668999272No Hit
TTGACATACTTTCCAATCAATAGGCCGGTTAACAGGGAGTTTTCTAAAAC6550.1185610489847192No Hit
ATCTAGAAGATCTCTTACTGAAGGAAAGAAATCAGAAGGCAAAAACGAGA6490.11747499357417217No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT6480.11729398433908099No Hit
CTTTTATATAAGATGCCCGCCTTCCATAGGGTATGTAAATAGTGTCTGGT6460.11693196586889862No Hit
ATGTAGCTCTTGTTCCCAAGAATAAGGTGACCCACAAAATGAGGCGCTAC6460.11693196586889862No Hit
TCCACAGGGTATGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTACA6450.11675095663380745No Hit
CGTAAAGAGAGGTGCGCCCCGTGGTCGGTTGGAACGGCAGACGGAGAAGG6440.11656994739871628No Hit
ACATAACCCATGAAATGTAGGGAATATCCCCATCTTTTTGTTTTGTTGGG6410.11602691969344275No Hit
TCTCTGGATAATAAGGTTTTATACCTTTCTCTAGGGGCAAATATTTTGTA6360.11512187351798689No Hit
CAATAGGCCGGTTAACAGGGAGTTTTCTAAAACAGTCTTTGATTTTCATT6290.11385480887234868No Hit
TCCTCTAACAGTAAATGAAAAACGAAGATTAAACTTAGTCATGCCTGCTA6260.11331178116707513No Hit
GATTAAACTTAGTCATGCCTGCTAGATTTTTTCCCATCTCTACAAAATAT6240.11294976269689279No Hit
TCCTAGTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGT6160.1115016888161634No Hit
GTGGATATCCTGCTTTAATGCCTCTGTATGCGTGTATTCAATCTAAGCAG6130.11095866111088988No Hit
GAATAAGGTGACCCACAAAATGAGGCGCTACGTGTAGTTTCTCTTTTATA6130.11095866111088988No Hit
GTTAGTATTCCTTGGACTCACAAGGTGGGAAATTTTACGGGGCTTTACTC6100.11041563340561636No Hit
GATTAACTACATTCTCTGGATAATAAGGTTTTATACCTTTCTCTAGGGGC6080.11005361493543402No Hit
GTCCCGTACTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC6070.10987260570034284No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTTGGAACGG6060.10969159646525167No Hit
GTGCAGGGTCCCCAATCCTCGAGAAGATTGACGAGATGAGTGAGGCAGTA6030.10914856875997812No Hit
GTATAGTAGAAGAGTAAAGCCCCGTAAAATTTCCCACCTTGTGAGTCCAA6020.10896755952488696No Hit
ATGTGGATATCCTGCTTTAATGCCTCTGTATGCGTGTATTCAATCTAAGC6000.1086055410547046No Hit
CCCATGAAATGTAGGGAATATCCCCATCTTTTTGTTTTGTTGGGATTTAA5990.10842453181961344No Hit
GTCTTTAATCCTAACTGGAAAACTCCATCTTTTCCTGATATTCATTTGCA5990.10842453181961344No Hit
GAATTATCTGTCCCGATAAGTTTCGCTCCAGACCGGCTGCGAGCAAAACA5960.10788150411433992No Hit
TCCTCAGGCCATGCAGTGGAATTCCACAACATTCCACCAAGCTCTGCAGG5960.10788150411433992No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5890.1066144394687017No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCGG5890.1066144394687017No Hit
CATTAAAGCAGGATATCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAA5850.10589040252833698No Hit
AATGTGAACAATTTGTAGGTCCTCTAACAGTAAATGAAAAACGAAGATTA5820.10534737482306347No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5810.1051663655879723No Hit
CACCAGGACATTATTAACAAATGTGAACAATTTGTAGGTCCTCTAACAGT5790.10480434711778995No Hit
GCACCAGGACATTATTAACAAATGTGAACAATTTGTAGGTCCTCTAACAG5780.10462333788269877No Hit
TAATAAGGTTTTATACCTTTCTCTAGGGGCAAATATTTTGTAGAGATGGG5770.1044423286476076No Hit
GTCGTTGACATTGCAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG5720.10353728247215173No Hit
GTATGCGTGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA5680.10281324553178704No Hit
GGTTAACAGGGAGTTTTCTAAAACAGTCTTTGATTTTCATTCTGATGTGT5680.10281324553178704No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5680.10281324553178704No Hit
GTACAACATCTTGAGTCCCTTTATACCTCTGTTACCAATTTTCTTTTGTC5660.1024512270616047No Hit
ATTAACAAATGTGAACAATTTGTAGGTCCTCTAACAGTAAATGAAAAACG5660.1024512270616047No Hit
TTATACCTCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAATC5660.1024512270616047No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGAGGGACGTCCTTTGTCTA5630.10190819935633115No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5630.10190819935633115No Hit
GTCTTTGGGTATACATTTAAATCCCAACAAAACAAAAAGATGGGGATATT5610.1015461808861488No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5580.10100315318087529No Hit
CCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG5560.10064113471069294No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)