FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488075

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488075
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences552458
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACTATACCTGTCTTTAATCCTAACTGGAAAACTCCATCTTTTCCTGAT32130.5815826723479433No Hit
GTTTTATACCTTTCTCTAGGGGCAAATATTTTGTAGAGATGGGAAAAAAT21570.3904369200916631No Hit
TCCATAGGGTATGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTACA21090.3817484768072867No Hit
ATGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTACATTCTCTGGAT21070.38138645833710433No Hit
ACTATACCTGTCTTTAATCCTAACTGGAAAACTCCATCTTTTCCTGATAT19410.3513389253119694No Hit
TTCTAAAACAGTCTTTGATTTTCATTCTGATGTGTTCCTGTGGTAATGAC18420.33341901103794314No Hit
ATTTGTAGGTCCTCTAACAGTAAATGAAAAACGAAGATTAAACTTAGTCA15690.28400348985805257No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCTCCCGTG15640.2830984436825967No Hit
CTCTTCTACTATACCTGTCTTTAATCCTAACTGGAAAACTCCATCTTTTC15590.28219339750714084No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC15100.27332394498767326No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCGTTGACATACT14860.2689797233454851No Hit
CTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAATCCCAACAAA14650.26517852940857045No Hit
GGATAATAAGGTTTTATACCTTTCTCTAGGGGCAAATATTTTGTAGAGAT14580.2639114647629322No Hit
TTATTAACAAATGTGAACAATTTGTAGGTCCTCTAACAGTAAATGAAAAA14230.2575761415347411No Hit
TGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTACATTCTCTGGATA13940.25232687371709706No Hit
CTTTAATCCTAACTGGAAAACTCCATCTTTTCCTGATATTCATTTGCACC13940.25232687371709706No Hit
ATTTTGTAGAGATGGGAAAAAATCTAGCAGGCATGACTAAGTTTAATCTT13910.2517838460118235No Hit
CTCTAACAGTAAATGAAAAACGAAGATTAAACTTAGTCATGCCTGCTAGA13420.242914393492356No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC13390.24237136578708246No Hit
CTTCTACTATACCTGTCTTTAATCCTAACTGGAAAACTCCATCTTTTCCT13180.2385701718501678No Hit
CAATTCGTTGACATACTTTCCAATCAATAGGCCGGTTAACAGGGAGTTTT12980.23494998714834434No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12860.2327778763272502No Hit
GTATGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTACATTCTCTGG12410.22463246074814736No Hit
CTTTATACCTCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA12250.22173631298668858No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11500.2081606203548505No Hit
CATTTAAATCCCAACAAAACAAAAAGATGGGGATATTCCCTACATTTCAT10870.1967570385441065No Hit
CTATTTACATACCCTATGGAAGGCGGGCATCTTATATAAAAGAGAAACTA10870.1967570385441065No Hit
ACTACATTCTCTGGATAATAAGGTTTTATACCTTTCTCTAGGGGCAAATA10850.19639502007392415No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT10490.1898786876106419No Hit
CTGTTAGAGGACCTACAAATTGTTCACATTTGTTAATAATGTCCTGGTGC10170.18408639208772432No Hit
GGGTATGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTACATTCTCT9960.18028519815080968No Hit
CCCTATGGAAGGCGGGCATCTTATATAAAAGAGAAACTACACGTAGCGCC9810.17757005962444206No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA9650.17467391186298326No Hit
CTACAAAATATTTGCCCCTAGAGAAAGGTATAAAACCTTATTATCCAGAG9510.1721397825717068No Hit
GGACATTATTAACAAATGTGAACAATTTGTAGGTCCTCTAACAGTAAATG9430.1706917086909774No Hit
GCTTTACTCTTCTACTATACCTGTCTTTAATCCTAACTGGAAAACTCCAT9420.17051069945588623No Hit
GTTTTAGAAAACTCCCTGTTAACCGGCCTATTGATTGGAAAGTATGTCAA9370.16960565328043037No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTCTGTATGCGTGTATT9260.16761455169442746No Hit
CCTTTATACCTCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA9030.16345133928733044No Hit
TGTTAATAATGTCCTGGTGCAAATGAATATCAGGAAAAGATGGAGTTTTC9020.16327033005223926No Hit
TTCTTGGACGGTCCCTCTCGAATGGGGGAAGAATCATTCCACCACCAATC8920.1614602377013275No Hit
CCTACAAATTGTTCACATTTGTTAATAATGTCCTGGTGCAAATGAATATC8530.15440087753277174No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT8280.14987564665549236No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGAGGGACGTCCTTTGTCTA8210.14860858200985413No Hit
ATGTAAGACCTTGGGCAATATTTGGTGGGCGTTCACGGTGGTCTCCATGC8170.14788454506948945No Hit
GTCCAAGAAAGCCCCATGATGTAGCTCTTGTTCCCAAGAATAAGGTGACC8140.14734151736421594No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAATATTTGGTGGGCGTTC8000.14480738807293947No Hit
TAACAAATGTGAACAATTTGTAGGTCCTCTAACAGTAAATGAAAAACGAA7800.14118720337111598No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGGAAGGCCTTGTAAGTTGGCGA7730.1399201387254778No Hit
GTTAGAGGACCTACAAATTGTTCACATTTGTTAATAATGTCCTGGTGCAA7680.1390150925500219No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGATTTCTTTCCTTCAGTAAG7650.13847206484474836No Hit
CTTTTATATAAGATGCCCGCCTTCCATAGGGTATGTAAATAGTGTCTGGT7620.13792903713947485No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCTCTGTTACCAATTTTCTT7490.1355759170832896No Hit
CATTTGCACCAGGACATTATTAACAAATGTGAACAATTTGTAGGTCCTCT7420.13430885243765137No Hit
GCCTAGCAGCCATGGAAAGGATGTATATTTCCGGGAAAGAACGACAGAAT7380.13358481549728668No Hit
TATCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCG7330.13267976932183081No Hit
GTCTTTAATCCTAACTGGAAAACTCCATCTTTTCCTGATATTCATTTGCA7210.1305076585007367No Hit
GTACGGGACCCTGCCGAACCTGCACGACTCTTGCTCAAGGAACCTCTATG7080.12815453844455144No Hit
GAATAAGGTGACCCACAAAATGAGGCGCTACGTGTAGTTTCTCTTTTATA7050.1276115107392779No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG7020.1270684830340044No Hit
GTTAGTATTCCTTGGACTCACAAGGTGGGAAATTTTACGGGGCTTTACTC6880.12453435374272796No Hit
ATGTAGCTCTTGTTCCCAAGAATAAGGTGACCCACAAAATGAGGCGCTAC6810.12326728909708973No Hit
GATTAAACTTAGTCATGCCTGCTAGATTTTTTCCCATCTCTACAAAATAT6780.1227242613918162No Hit
GTATAGTAGAAGAGTAAAGCCCCGTAAAATTTCCCACCTTGTGAGTCCAA6710.121457196746178No Hit
ATTCCCTACATTTCATGGGTTATGTAATTGGAAGTTGGGGGTCATTACCA6650.12037114133563094No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACC6610.11964710439526624No Hit
ATGTAGGGAATATCCCCATCTTTTTGTTTTGTTGGGATTTAAATGTATAC6460.11693196586889862No Hit
CACGCATACAGAGGCATTAAAGCAGGATATCCACATTGTGTAAAAGGGGC6370.11530288275307807No Hit
GTCTTTGGGTATACATTTAAATCCCAACAAAACAAAAAGATGGGGATATT6320.11439783657762219No Hit
GTCCCGTACTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC6250.11313077193198395No Hit
GGTTAACAGGGAGTTTTCTAAAACAGTCTTTGATTTTCATTCTGATGTGT6240.11294976269689279No Hit
GTAGGGAATATCCCCATCTTTTTGTTTTGTTGGGATTTAAATGTATACCC6230.11276875346180161No Hit
GTATGCGTGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA6160.1115016888161634No Hit
TTTCATGGGTTATGTAATTGGAAGTTGGGGGTCATTACCACAGGAACACA6150.11132067958107222No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC6110.11059664264070754No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6090.11023462417052518No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG6050.10951058723016047No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6010.10878655028979578No Hit
GTATAAAACCTTATTATCCAGAGAATGTAGTTAATCATTACTTCCAAACC6000.1086055410547046No Hit
GTGCAAGGTCCCCAATCCTCGAGAAGATTGACGAGATGAGTGAGGCAGTA5990.10842453181961344No Hit
CAATAGGCCGGTTAACAGGGAGTTTTCTAAAACAGTCTTTGATTTTCATT5990.10842453181961344No Hit
GTGCAGGGTCCCCAATCCTCGAGAAGATTGACGAGATGAGTGAGGCAGTA5960.10788150411433992No Hit
TCCTAGTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGT5950.10770049487924874No Hit
TTGACATACTTTCCAATCAATAGGCCGGTTAACAGGGAGTTTTCTAAAAC5940.10751948564415757No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5930.1073384764090664No Hit
GATTAACTACATTCTCTGGATAATAAGGTTTTATACCTTTCTCTAGGGGC5920.10715746717397522No Hit
CCTCTATGTTTCCCTCATGTTGCTGTTCAAAACCTTCGGACGGAAATTGC5890.1066144394687017No Hit
GAATTATCTGTCCCGATAAGTTTCGCTCCAGACCGGCTGCGAGCAAAACA5860.10607141176342819No Hit
ATGTGGATATCCTGCTTTAATGCCTCTGTATGCGTGTATTCAATCTAAGC5830.10552838405815464No Hit
GCACCAGGACATTATTAACAAATGTGAACAATTTGTAGGTCCTCTAACAG5800.10498535635288112No Hit
GTGGATATCCTGCTTTAATGCCTCTGTATGCGTGTATTCAATCTAAGCAG5750.10408031017742525No Hit
GCTTTAATGCCTCTGTATGCGTGTATTCAATCTAAGCAGGCTTTCACTTT5710.10335627323706056No Hit
TCCCATCAGCGCTGAATCCTGCGGACGACCCGTCTCGGGGTCGCTTGGGG5710.10335627323706056No Hit
TCTCTGGATAATAAGGTTTTATACCTTTCTCTAGGGGCAAATATTTTGTA5700.10317526400196939No Hit
CACCAGGACATTATTAACAAATGTGAACAATTTGTAGGTCCTCTAACAGT5690.10299425476687821No Hit
CATTAAAGCAGGATATCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAA5630.10190819935633115No Hit
TCCACAGGGTATGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTACA5620.10172719012123997No Hit
CTCTAGGGGCAAATATTTTGTAGAGATGGGAAAAAATCTAGCAGGCATGA5550.10046012547560176No Hit
ACATAACCCATGAAATGTAGGGAATATCCCCATCTTTTTGTTTTGTTGGG5550.10046012547560176No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)