FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488076

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488076
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414340
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24510.5915431771009316No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA16490.3979823333494232No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT15800.38132934305160016No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC10860.2621035864266062No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT10520.2538977651204325No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA10420.25148428826567554No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG10340.24955350678186997No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT9770.2357966887097553No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT9720.2345899502823768No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTTAT9490.22903895351643577No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA9480.2287976058309601No Hit
ACCTCGCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGACATTCATT9470.22855625814548441No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA9420.2273495197181059No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT9410.2271081720326302No Hit
CCCTTACAGTAAATGAAAACAGGAGATTAAAATTAATTATGCCTGCTAGG8330.201042622001255No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG7630.18414828401795627No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGAAAACTTCCTGTAA7590.18318289327605347No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGTGGGTCACCATATT7270.1754597673408312No Hit
GTTTACAGGAAGTTTTCTAAAACACATTTTGATTTTTTGTACAATATGTT7060.1703914659458416No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATAAA6880.16604720760727906No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC6830.16484046917990056No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT6810.16435777380894917No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG6460.1559106048172998No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC6370.15373847564801854No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG6260.15108365110778588No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTCTGGA6210.1498769126804074No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC6060.14625669739827196No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC6010.14504995897089348No Hit
CAATAGGCCTGTTTACAGGAAGTTTTCTAAAACACATTTTGATTTTTTGT5990.14456726359994207No Hit
GGGTTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAATTTGGAAGAT5920.1428778298016122No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA5910.1426364821161365No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC5880.1419124390597094No Hit
GGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGA5850.1411883960032823No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATACGCATGTATACAAGCAAAAC5820.14046435294685525No Hit
TAGATAAAGGGATCAAACCTTATTATCCAGAGCATGTAGTTAATCATTAC5810.14022300526137954No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG5650.1363614422937684No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCGACTTATAAGTCC5640.1361200946082927No Hit
GTGGATATCCTGCTTTAATGCCTTTATACGCATGTATACAAGCAAAACAG5620.1356373992373413No Hit
GTTCAGATACTGTTTACTTAGAAAGGACTTATAAGTCGGCGAGAAAGTAA5560.13418931312448715No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA5510.13298257469710864No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA5490.13249987932615728No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACGATGTCCTCCTGC5450.13153448858425448No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5340.12887966404402182No Hit
GGGTAGAGGTTCAGATACTGTTTACTTAGAAAGGACTTATAAGTCGGCGA5290.12767292561664334No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG5280.12743157793116763No Hit
GAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACA5240.12646618718926486No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5220.12598349181831345No Hit
AAGCAAGGTACCGTAGAAGAATAAAGCCCCGTAAAGTTTCCCACCTTATG5130.1238113626490322No Hit
ACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGACATTCATT5120.12357001496355649No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT5110.1233286672780808No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT5100.12308731959260512No Hit
GTATACATGCGTATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA5060.12212192885070232No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA5000.12067384273784815No Hit
GTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG4910.11850171356856688No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4900.11826036588309119No Hit
GCACATCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGAC4810.11608823671380993No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC4780.11536419365738282No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA4730.11415745523000434No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4690.11319206448810155No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC4690.11319206448810155No Hit
CCTTTATACGCATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCGAC4660.11246802143167448No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT4570.11029589226239321No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA4540.1095718492059661No Hit
ATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAACCTTAT4540.1095718492059661No Hit
ACTACATGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATA4500.10860645846406333No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4420.10667567698025776No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA4390.10595163392383067No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG4270.10305546169812232No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT4250.10257276632717093No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCCGTCCCGATGAGTTT4250.10257276632717093No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA4240.10233141864169522No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT4220.10184872327074383No Hit
CCCAATTACATATCCCATAAAGTTAAGGGAATATCCCCATCTTTTTGTTT4220.10184872327074383No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA4210.10160737558526814No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA4180.10088333252884106No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)