FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414340
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24550.5925085678428343No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT15730.3796399092532703No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA15600.37650238934208624No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT10940.26403436791041174No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG10440.2519669836366269No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC10310.24882946372544287No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA9900.23893420862093934No Hit
ACCTCGCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGACATTCATT9890.2386928609354636No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT9260.22348795675049474No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA9170.22131582758121351No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA8650.20876574793647729No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT8590.20731766182362313No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT8210.19814644977554666No Hit
CCCTTACAGTAAATGAAAACAGGAGATTAAAATTAATTATGCCTGCTAGG8170.19718105903364386No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTTAT7950.19187140995317856No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATAAA7590.18318289327605347No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG7410.17883863493749094No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT6930.16725394603465754No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGAAAACTTCCTGTAA6860.16556451223632765No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGTGGGTCACCATATT6830.16484046917990056No Hit
GTTTACAGGAAGTTTTCTAAAACACATTTTGATTTTTTGTACAATATGTT6480.1563933001882512No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC6460.1559106048172998No Hit
GGGTTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAATTTGGAAGAT6340.15301443259159145No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC6220.15011826036588308No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG6210.1498769126804074No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG6130.14794613119660183No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA5920.1428778298016122No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATACGCATGTATACAAGCAAAAC5880.1419124390597094No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA5850.1411883960032823No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC5820.14046435294685525No Hit
GGGTAGAGGTTCAGATACTGTTTACTTAGAAAGGACTTATAAGTCGGCGA5790.13974030989042815No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTCTGGA5780.13949896220495248No Hit
GTATACATGCGTATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA5700.13756818072114688No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACGATGTCCTCCTGC5670.1368441376647198No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5600.13515470386638992No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC5580.13467200849543853No Hit
CAATAGGCCTGTTTACAGGAAGTTTTCTAAAACACATTTTGATTTTTTGT5490.13249987932615728No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG5480.13225853164068158No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCGACTTATAAGTCC5470.13201718395520587No Hit
AGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTACGTCC5460.13177583626973016No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5440.1312931408987788No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC5440.1312931408987788No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC5410.13056909784235168No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA5340.12887966404402182No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA5300.12791427330211902No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT5250.12670753487474054No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG5160.12453540570545928No Hit
GTGGATATCCTGCTTTAATGCCTTTATACGCATGTATACAAGCAAAACAG5150.1242940580199836No Hit
TAGATAAAGGGATCAAACCTTATTATCCAGAGCATGTAGTTAATCATTAC5140.1240527103345079No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT5080.12260462422165372No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5070.12236327653617801No Hit
ACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGACATTCATT4960.11970845199594535No Hit
GTTCAGATACTGTTTACTTAGAAAGGACTTATAAGTCGGCGAGAAAGTAA4860.1172949751411884No Hit
GTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG4810.11608823671380993No Hit
GGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGA4790.11560554134285853No Hit
AAGCAAGGTACCGTAGAAGAATAAAGCCCCGTAAAGTTTCCCACCTTATG4760.11488149828643145No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA4720.11391610754452863No Hit
GAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACA4680.11295071680262586No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4640.11198532606072308No Hit
ACCTTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATT4590.1107785876333446No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT4570.11029589226239321No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT4560.11005454457691752No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4560.11005454457691752No Hit
ATTTGGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA4530.10933050152049041No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT4530.10933050152049041No Hit
TCCCTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG4320.1042622001255008No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGATTA4290.1035381570690737No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG4290.1035381570690737No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC4250.10257276632717093No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA4250.10257276632717093No Hit
CCACAAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG4190.10112468021431674No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC4180.10088333252884106No Hit
CCTTTATACGCATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCGAC4160.10040063715788966No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA4150.10015928947241395No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)