FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369967
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN60711.6409571664499807No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA13720.37084388607632573No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT10420.2816467414661307No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTGCCTCAA9730.26299642941127177No Hit
GTGTCAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC9630.26029348563520527No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA9150.24731935551008605No Hit
CCTCCTGCTTACAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA9140.24704906113247938No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAGAAGTA8630.2332640478745402No Hit
CATTTGAACCCTAATAAAACTAAACGTTGGGGATACTCCCTTAACTTCAT8470.2289393378328337No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTGAGTACAATA8320.22488492216873399No Hit
GTATACATGCATATAAAGGCATTAGGGCAGGATAGCCACATTGTGTAAAA7640.20650490449148168No Hit
ATGTTAATATGGGCCTAAAGATCAGACAACTATTGTGGTTTCACATTTCC7470.2019099000721686No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT7180.19407136312157572No Hit
GTTAAGGGAGTATCCCCAACGTTTAGTTTTATTAGGGTTCAAATGTATAC6800.18380017677252294No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTATTCG6660.18001605548602984No Hit
ATTTTGTGGTCTGTAAGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA6600.1783942892203899No Hit
GTACAATATGTTCTTGAGGCAAAGTACCCCAACTTCCAATTACATATCCC6480.17515075668911012No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6270.16947457475937042No Hit
GTAAAACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGCGA6170.16677163098330391No Hit
CCTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCT6160.16650133660569727No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG6150.16623104222809063No Hit
GGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTGAGTACAAT5960.16109544905356424No Hit
GTGTATACATGCATATAAAGGCATTAGGGCAGGATAGCCACATTGTGTAA5910.15974397716553096No Hit
CAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTGAGT5890.15920338841031767No Hit
CTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAAC5880.15893309403271103No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC5730.15487867836861124No Hit
TGTATACACTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTT5730.15487867836861124No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5570.15055396832690482No Hit
AGTTAAGGGAGTATCCCCAACGTTTAGTTTTATTAGGGTTCAAATGTATA5530.1494727908164782No Hit
TAATAAAACTAAACGTTGGGGATACTCCCTTAACTTCATGGGATATGTAA5370.14514808077477181No Hit
GCCTAGTCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5320.14379660888673854No Hit
ATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTGAGTACAATATGTT5190.14028278197785207No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA5190.14028278197785207No Hit
GTATACATTTGAACCCTAATAAAACTAAACGTTGGGGATACTCCCTTAAC5170.13974219322263878No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5130.13866101571221218No Hit
CATGTATACACTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5130.13866101571221218No Hit
GTCCCGTGCTGGTAGTTGATGTTCATTAAGATTCCCGAGATTGAGATCTT5080.13730954382417893No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5070.13703924944657228No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACTAAACGTTGGGGATACT4960.1340660112928991No Hit
CTTTTACACAATGTGGCTATCCTGCCCTAATGCCTTTATATGCATGTATA4950.13379571691529243No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTCC4930.13325512816007914No Hit
ATGTAATTGGAAGTTGGGGTACTTTGCCTCAAGAACATATTGTACTCAAA4890.13217395064965254No Hit
CCTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA4820.13028189000640597No Hit
ATATTGTACTCAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGA4810.13001159562879933No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA4800.12974130125119268No Hit
GTTAATATGGGCCTAAAGATCAGACAACTATTGTGGTTTCACATTTCCTG4780.12920071249597936No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG4780.12920071249597936No Hit
GGCCTAAAGATCAGACAACTATTGTGGTTTCACATTTCCTGTCTTACTTT4750.12838982936315943No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT4720.12757894623033947No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4700.12703835747512618No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGGCGATGGCC4690.1267680630975195No Hit
CTCCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTGCCTC4590.124065119321453No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4510.12190276430059978No Hit
GTCGATGAGATCTCGAATAGAAGGAAAAAAGTCAGAAGGCAAAAAAGAGA4430.11974040927974657No Hit
CAATATGTTCTTGAGGCAAAGTACCCCAACTTCCAATTACATATCCCATG4420.11947011490213992No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4390.11865923176931996No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4370.11811864301410667No Hit
ATTGTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA4340.11730775988128672No Hit
GGATGGGGCTTGGCTATTGGCCATCGCCGCATGCGTGGAACCTTTGTGGC4330.11703746550368005No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG4280.1156859936156468No Hit
GAATTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAGATCAGACA4260.11514540486043351No Hit
CCACAAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG4250.11487511048282684No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA4240.11460481610522019No Hit
GGGAATTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAGATCAGA4130.11163157795154703No Hit
ATATGTTCTTGAGGCAAAGTACCCCAACTTCCAATTACATATCCCATGAA4130.11163157795154703No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT4080.11028010606351378No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG4010.10838804542026721No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAGAGAATTGTGGGTCTTTTGG4000.10811775104266057No Hit
GCTTAGAGTGTATACATGCATATAAAGGCATTAGGGCAGGATAGCCACAT3940.10649598477702066No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCTTACAGACCACAAAATGCC3940.10649598477702066No Hit
CTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGT3910.1056851016442007No Hit
GGATATGTAATTGGAAGTTGGGGTACTTTGCCTCAAGAACATATTGTACT3900.10541480726659405No Hit
GGGTATACATTTGAACCCTAATAAAACTAAACGTTGGGGATACTCCCTTA3870.10460392413377409No Hit
CTTTATACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA3840.10379304100095413No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT3830.10352274662334748No Hit
CTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTC3820.10325245224574084No Hit
TACATATCCCATGAAGTTAAGGGAGTATCCCCAACGTTTAGTTTTATTAG3820.10325245224574084No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGATGAGATCTCGAA3810.10298215786813418No Hit
GCTATGTCAATGTTAATATGGGCCTAAAGATCAGACAACTATTGTGGTTT3810.10298215786813418No Hit
CTTTAGGCCCATATTAACATTGACATAGCTGACTACTAATTCCCTGGATG3790.10244156911292089No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA3730.10081980284728097No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC3700.100008919714461No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)