FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences599075
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26840.44802403705712973No Hit
CTACTATACCTGTCTTTAATCCTAACTGGAAAACTCCATCTTTTCCTGAT23890.3987814547427283No Hit
ACTATACCTGTCTTTAATCCTAACTGGAAAACTCCATCTTTTCCTGATAT14980.2500521637524517No Hit
GTTTTATACCTTTCTCTAGGGGTAAATATTTCGTAGAGATAGGAAAAAAT14900.24871677168968828No Hit
CCCTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTTTTGCCTT14260.23803363518758086No Hit
GTGTAAAAGGGGCAGCAAAGCCCAACAGACCCACAATTCTTTGACATACT13840.23102282685807285No Hit
TTCTAAAACAGTCTTTGATTTTCATTCTGATGTGTTCCTGTGGTAATGAC12950.21616659015982975No Hit
CTCTTCTACTATACCTGTCTTTAATCCTAACTGGAAAACTCCATCTTTTC12730.21249426198723032No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTAAG12690.21182656595584862No Hit
ATGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTACATTCTCTGGAT12680.21165964194800316No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA12330.2058173016734132No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC12110.20214497350081376No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTCTGTATGCGTGTATT11910.1988064933439052No Hit
GTTTTAGAAAACTCCCTGTTAATCGGCCTATTGATTGGAAAGTATGTCAA11720.19563493719484204No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCTCCCGTG11710.1954680131869966No Hit
CTTTAATCCTAACTGGAAAACTCCATCTTTTCCTGATATTCATTTGCACC11710.1954680131869966No Hit
CTATTTACATACCCTATGGAAGGCGGGCATCTTATATAAAAGGGAAACTA11520.19229645703793347No Hit
GTCTTACACAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT11230.18745566081041606No Hit
TCCATAGGGTATGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTACA10970.18311563660643493No Hit
ATTTGTAGGTCCTCTAACAGTAAATGAAAAACGAAGATTAAACTTAGTCA10260.17126403204940951No Hit
CTTCTACTATACCTGTCTTTAATCCTAACTGGAAAACTCCATCTTTTCCT10230.17076326002587322No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9950.16608938780620122No Hit
CTGTTAGAGGACCTACAAATTGTTCACATTTGTTAATAATGTCCTGGTGC9820.16391937570421067No Hit
GGATAATAAGGTTTTATACCTTTCTCTAGGGGTAAATATTTCGTAGAGAT9800.1635855276885198No Hit
CTCTAGGGGTAAATATTTCGTAGAGATAGGAAAAAATCTAGCAGGCATGA9640.16091474356299296No Hit
GTCCAAGAAAGCCCCATGATGTAGCTCTTGTTCCCAAGAATAAGGTGAAC9620.1605808955473021No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGAGGGACGTCCTTTGTCTA9490.1584108834453115No Hit
GTGTAAGACCTTGGGCAAGATTTGGTGGGCGTTCACGGTGGTCTCCATGC9400.15690856737470268No Hit
GTACGGGACCCTGCCGAACCTGCACGACTCTTGCTCAAGGAACCTCTATG9070.15140007511580353No Hit
CCTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA8600.1435546467470684No Hit
TTATTAACAAATGTGAACAATTTGTAGGTCCTCTAACAGTAAATGAAAAA8540.14255310269999583No Hit
CTCTAACAGTAAATGAAAAACGAAGATTAAACTTAGTCATGCCTGCTAGA8540.14255310269999583No Hit
CTTTAGGACATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGGA8530.1423861786921504No Hit
TGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTACATTCTCTGGATA8490.14171848266076867No Hit
ACTACATTCTCTGGATAATAAGGTTTTATACCTTTCTCTAGGGGTAAATA8480.14155155865292327No Hit
GTTCAAAACCTTCGGACGGAAATTGCACTTGTATTCCCATCCCATCATCA8420.1405500146058507No Hit
CTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA8370.13971539456662355No Hit
GCTTTACTCTTCTACTATACCTGTCTTTAATCCTAACTGGAAAACTCCAT8200.13687768643325127No Hit
CCTACAAATTGTTCACATTTGTTAATAATGTCCTGGTGCAAATGAATATC8000.1335392062763427No Hit
TTGACATACTTTCCAATCAATAGGCCGATTAACAGGGAGTTTTCTAAAAC7900.1318699661978884No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG7830.1307014981429704No Hit
TATCCACATTGTGTAAAAGGGGCAGCAAAGCCCAACAGACCCACAATTCT7720.1288653340566707No Hit
ATGTGGATATCCTGCTTTAATGCCTCTGTATGCGTGTATTCAATCTAAGC7680.128197638025289No Hit
TTCTTGGACGGTCCCTCTCGAATGGGGGAAGAATCATTCCACCACCAATC7570.12636147393898928No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCAAAGCCCAACAGACCCACAAT7480.12485915786838042No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC7430.12402453782915326No Hit
GATGTGTTCCTGTGGTAATGACCCCCAACTTCCAATTACATAACCCATGA7430.12402453782915326No Hit
GTATGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTACATTCTCTGG7410.12369068981346243No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCT7400.123523765805617No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7400.123523765805617No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGG7380.12318991778992613No Hit
GCTTTAATGCCTCTGTATGCGTGTATTCAATCTAAGCAGGCTTTCACTTT7350.12268914576638984No Hit
CATTTAAATCCCAACAAAACAAAAAGATGGGGATATTCCCTAAATTTCAT7340.12252222175854442No Hit
GCCTAGCAGCCATGGAAATGATGTATATTTTCGGGAGAGAACGACAGAAT7240.12085298168009014No Hit
CTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTTTTGCCTTCT7230.1206860576722447No Hit
CCCTATGGAAGGCGGGCATCTTATATAAAAGGGAAACTACACGTAGCGCC7150.11935066560948128No Hit
ATGACTAACTACATTCTCTGGATAATAAGGTTTTATACCTTTCTCTAGGG7130.11901681759379043No Hit
GTGGATATCCTGCTTTAATGCCTCTGTATGCGTGTATTCAATCTAAGCAG7100.11851604557025415No Hit
GGTCCATGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG7010.1170137294996453No Hit
GGACATTATTAACAAATGTGAACAATTTGTAGGTCCTCTAACAGTAAATG7000.11684680549179985No Hit
ATGTAGCTCTTGTTCCCAAGAATAAGGTGAACCAAAAAATGAGGCGCTAC6990.11667988148395443No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCTCTATTACCAATTTTCTT6960.11617910946041814No Hit
GTATGCGTGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA6850.11434294537411842No Hit
GTTAGAGGACCTACAAATTGTTCACATTTGTTAATAATGTCCTGGTGCAA6800.11350832533489129No Hit
ACATTGACCCTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTT6670.11133831323290072No Hit
ATCTAGAAGATCTCTTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA6570.10966907315444643No Hit
GTATGGTGAGGTGTACAATGCTCAGGAGATTCTAAGGCATCCCGATACAG6560.109502149146601No Hit
TGTTAATAATGTCCTGGTGCAAATGAATATCAGGAAAAGATGGAGTTTTC6430.10733213704461045No Hit
GTCCAAGAGTCCTCTTGTGTAAGACCTTGGGCAAGATTTGGTGGGCGTTC6170.1029921128406293No Hit
GTCCCGTACTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC6150.10265826482493845No Hit
GTATAGTAGAAGAGTAAAGCCCCGTAAAATTTCCCACCTTGTGAGTCCAA6150.10265826482493845No Hit
GAATAAGGTGAACCAAAAAATGAGGCGCTACGTGTAGTTTCCCTTTTATA6090.10165672077786587No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC6050.10098902474648416No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG6030.1006551767307933No Hit
TAACAAATGTGAACAATTTGTAGGTCCTCTAACAGTAAATGAAAAACGAA6010.10032132871510245No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)