FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488084

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488084
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences581087
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN52640.9058884469967492No Hit
GCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA40020.688709263845173No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT32490.5591245372895969No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT18770.3230153144021463No Hit
CTCTTATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGT18280.31458284215616594No Hit
CTTATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTT17920.3083875564244253No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCCCTAAATTTTAT17900.308043373883773No Hit
CATTTACATCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT16850.2899737904995293No Hit
TAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGT15210.26175082216604395No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT14980.257792722948543No Hit
CATCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACTCACAGT14950.2572764491375646No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGT14860.2557276277046294No Hit
CTACTGTACCTGTCTTTAATCCTAATTGGAAAACACCCACTTTTCCTAAT14620.25159743721680233No Hit
ATCCCAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT14410.24798352053995357No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTGGGATAAAAC14220.2447137864037571No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT14140.24333705624114804No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTGGGATAAAACCTAGCAGG12910.22216982999103402No Hit
CAGTTAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTTTTAT12690.2183838220438592No Hit
CTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA12140.20891880217592204No Hit
GTTAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTTTTATCC11670.20083051247059389No Hit
GTTTTATCCCAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAAC11650.20048632992994164No Hit
ATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTTGGA11320.19480731800917936No Hit
GTACCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC11130.1915375838729829No Hit
CTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTG11100.19102131006200448No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCGTTGACACACT10750.18499811560058993No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10540.1813841989237412No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACTAGACACTATT10400.17897492113917537No Hit
CTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA9880.1700261750822166No Hit
GAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTTTTATCCCAATGTTA9470.16297043299884525No Hit
GTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGG9460.16279834172851915No Hit
CTCTAAAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA9390.16159370283623622No Hit
GATTATGCCTGCTAGGTTTTATCCCAATGTTACCAAATATTTGCCATTGG9030.15539841710449553No Hit
GTTTGTAGGCCCACTCACAGTTAATGAGAAAAGAAGATTGCAATTGATTA8740.15040777026503777No Hit
ATATTTGCCATTGGATAAGGGTATTAAACCTTATTATCCAGAACATCTAG8430.14507294088492773No Hit
AATATACATTTACATCAAGACATTATCAAAAAATGTGAACAGTTTGTAGG8210.14128693293775288No Hit
ATCCAGAACATCTAGTTAATCATTACTTCCAAACTAGACACTATTTACAC8080.13904974642351317No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA8030.13818929007188252No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7980.13732883372025187No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGTATGATGTGTT7830.13474746466535992No Hit
GTGTTGTTTCTCTCTTATATAATATACCCGCCTGCCATAGAGTGTGTAAA7770.13371491704340313No Hit
CTCACAGTTAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTT7540.12975681782590215No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACT7530.129584726555576No Hit
CCTACAAACTGTTCACATTTTTTGATAATGTCTTGATGTAAATGTATATT7450.12820799639296698No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT7440.12803590512264085No Hit
GCTTTATTCCTCTACTGTACCTGTCTTTAATCCTAATTGGAAAACACCCA7370.12683126623035793No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTGGGATAAAA7300.125626627338075No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGAACACATCATACAAAAA7230.12442198844579211No Hit
GGCATAATCAATTGCAATCTTCTTTTCTCATTAACTGTGAGTGGGCCTAC6930.1192592503360082No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT6930.1192592503360082No Hit
ACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTATTATCCAGAACA6920.11908715906568208No Hit
AATTTAGGGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATA6850.11788252017339916No Hit
CTCTACAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA6840.11771042890307304No Hit
TTCCTCTACTGTACCTGTCTTTAATCCTAATTGGAAAACACCCACTTTTC6710.11547324238883334No Hit
AGTCTGTACCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT6580.11323605587459365No Hit
ATAACATCCAATGACATAGCCCATAAAATTTAGGGAGTAACCCCATCTTT6560.11289187333394139No Hit
ACCCATAACATCCAATGACATAGCCCATAAAATTTAGGGAGTAACCCCAT6520.11220350825263688No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG6470.11134305190100623No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6250.10755704395383137No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCCCTAA6120.10531985743959167No Hit
CTTTAATCCTAATTGGAAAACACCCACTTTTCCTAATATACATTTACATC6010.10342685346600422No Hit
TTGTAGGCCCACTCACAGTTAATGAGAAAAGAAGATTGCAATTGATTATG5990.10308267092535198No Hit
GGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCCCTAAA5990.10308267092535198No Hit
ATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAG5980.10291057965502584No Hit
GGCAAATATTTGGTAACATTGGGATAAAACCTAGCAGGCATAATCAATTG5900.1015338494924168No Hit
GTTATGGGTCATTGCCACAAGAACACATCATACAAAAAATCAAAGAATGT5850.10067339314078616No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)