FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488089

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488089
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448130
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA29310.6540512797625688No Hit
ATCTCAATGTCAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG24370.5438154107067146No Hit
CAATTACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTTTG20450.4563407939660366No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA14520.3240131211925111No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT12930.2885323455247361No Hit
CAATGACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTTTG12840.28652399973222054No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG12440.27759801843215137No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12430.27737486889964963No Hit
TAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT12050.26889518666458395No Hit
CTCTTATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT11010.24568763528440407No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT10710.23899314930935217No Hit
CATCATACAGAAAATCAAAGAATGCTTTAGAAAACTTCCTGTTAACAGGC10610.2367616539843349No Hit
CATTTAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT10600.23653850445183316No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAACAAC10430.23274496239930378No Hit
ATGTAATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA10420.232521812866802No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC10180.22716622408676052No Hit
CTCTATGGAAGGCGGGTGTATTATATAAGAGAGAAACTACACATAGCGCC10160.22671992502175708No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC10080.22493472876174325No Hit
CTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAA10080.22493472876174325No Hit
ATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA9990.22292638296922768No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG9950.2220337848392208No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT9680.21600874746167406No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT9570.21355410260415503No Hit
ATGTAATTGGATGTTATGGGTCATTGCCACAAGATCACATCAGACAGAAA9470.21132260727913776No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT9380.2093142614866222No Hit
GCGTTCTATCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGT9210.20552071943409278No Hit
GATCCTCATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTTTTGCC8730.19480954187400976No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT8660.19324749514649767No Hit
ATCTAAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG8520.19012340169147346No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC7950.1774038783388749No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7930.17695757927387143No Hit
CTTTAGGGCATGGACATTGATCTTTATAAAGAATTTGGAGCTACTGTGGA7420.1655769531162832No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7080.15798986901122442No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT6980.15575837368620712No Hit
CTCTCTCGTCCCCTTCTCCGTCTGCCGTTTCGACCGACCACGGGGCGCAC6710.14973333630866045No Hit
GAATACATGCATACAAAGGCATTAAAGCGGGATAACCACATTGTGTAAAA6700.14951018677615868No Hit
CCAACATCCAGGGACCTAGTAGTCAGTTATGTCAACACTAATATGGGCCT6650.14839443911365005No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6410.14303885033360855No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6390.1425925512686051No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT6380.14236940173610338No Hit
GCCTACCCCGCTGTCTCCACCTTTGAGAGACACTCATCCTCAGGCCATGC6330.14125365407359472No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT6110.13634436435855668No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAGCATTCTTTGATTTTCTGT6000.13388971950103765No Hit
ATGTCAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTAT5970.13322027090353245No Hit
GATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAGTTGGAGGAC5960.13299712137103073No Hit
CTCTATAACTGTTTCTCTTCCAAAAGTTAGACAAGAAATGTGAAACCACA5900.13165822417602036No Hit
ATATAAGAGAGAAACTACACATAGCGCCTCATTTTGTGGGTCACCATATT5830.13009617744850824No Hit
CTTATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT5800.12942672885100306No Hit
GTTTTGTACAGCAACAGGAGGGATACATAGAGGTTCCTTGAGCAGGAGTC5690.12697208399348403No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5660.12630263539597886No Hit
GTACAAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC5660.12630263539597886No Hit
ATACGCTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCT5620.1254100372659719No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5580.12451743913596501No Hit
CCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACACCAAGACA5550.12384799053845982No Hit
CTGTCTGATGTGATCTTGTGGCAATGACCCATAACATCCAATTACATAGC5430.12117019614843907No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT5350.11938499988842523No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG5300.11826925222591658No Hit
GTTATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5290.11804610269341485No Hit
GCCTAGCAGCCATGGATACGATGTATATTTGCGGGAGAGAACAACAGAGT5290.11804610269341485No Hit
CTCACAGTCAATGAGAAAAGAAGACTGAAATTGATTATGCCTGCTAGGTT5220.11648405596590275No Hit
GATCTTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTTTTGCC5160.11514515877089238No Hit
CTTTTACACAATGTGGTTATCCCGCTTTAATGCCTTTGTATGCATGTATT5130.11447571017338719No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT5100.113806261575882No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACA4990.11135161671836297No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA4960.11068216812085778No Hit
GTGAAACACTCATCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACC4950.11045901858835606No Hit
ACAGTAGTCTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGGTCTATA4940.11023586905585432No Hit
GGATATTGGATCTTCCAAATTACCACCCACCCAGGTAGCTAGAGTCATTA4920.10978956999085088No Hit
CATTTGGATAAAACCTAGCAGGCATAATCAATTTCAGTCTTCTTTTCTCA4900.1093432709258474No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCAGACAGAAA4870.10867382232834222No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC4850.10822752326333876No Hit
CTCTCGTCCCCTTCTCCGTCTGCCGTTTCGACCGACCACGGGGCGCACCT4850.10822752326333876No Hit
GTGTAGTTTCTCTCTTATATAATACACCCGCCTTCCATAGAGTGTGTAAA4810.10733492513333184No Hit
CAATTAGGGTTAAAGACAGGTACAGTAGAAGAATAAAGCCCCGTAAAGTT4810.10733492513333184No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA4800.10711177560083013No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG4790.10688862606832839No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC4770.10644232700332493No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAA4680.10443398121080937No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4680.10443398121080937No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4670.10421083167830762No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4630.10331823354830072No Hit
GTTCCGGCAACTATTGTGGTTTCACATTTCTTGTCTAACTTTTGGAAGAG4620.10309508401579899No Hit
ATCCCGATTGGGACTTCAATCCCAACAAGGACACCTGGCCAGACGCCAAC4540.10130988775578514No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACT4540.10130988775578514No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)