FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences598750
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGTTT36370.6074321503131525No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27400.45762004175365345No Hit
GGATAATAAGGTTTAATCCCTTTGTCTAAAGGCAAATATTTTGTGCTGTT26660.44526096033402923No Hit
ATATGATCCTGTGGCAATGTACCCCAACTTCCAATTACATAACCCATGAA19870.33185803757828813No Hit
TTCTTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACCCCTTCTTTTC19770.33018789144050104No Hit
GCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGTAAATGAAAAAAG18920.31599164926931106No Hit
CCTATTAGCAATGTCTTCATGTAAATGAATTTTAGGAAAAGAAGGGGTTT17210.2874321503131524No Hit
CTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGT17070.28509394572025054No Hit
TTATTATCCTGATCATGTAGTTAATCATTACTTTCAAACCCGACATTATT16490.2754070981210856No Hit
GTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACATGATCAGG14850.24801670146137786No Hit
GTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA14810.24734864300626305No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCCCTGAATTTCAT14760.2465135699373695No Hit
CTCCGAAAGAGACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGG14250.23799582463465552No Hit
GGATCATATTGTACAAAAAATCAAACACTGTTTTAGAAAACTTCCTGTCA13410.22396659707724426No Hit
CATGAAGACATTGCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGT12890.21528183716075155No Hit
TCCAAAGAGTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACA12660.21144050104384132No Hit
GTCTAAAGGCAAATATTTTGTGCTGTTAGGATAAAACCTAGCAGGCATAA12660.21144050104384132No Hit
CTTTAATCCTGAATGGCAAACCCCTTCTTTTCCTAAAATTCATTTACATG11540.1927348643006263No Hit
GTGTAAATGGAGCGGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT11030.18421711899791232No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA10270.17152400835073067No Hit
TTCTAAAACAGTGTTTGATTTTTTGTACAATATGATCCTGTGGCAATGTA9430.1574947807933194No Hit
GTGTAGTTTCCCTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAA9380.1566597077244259No Hit
GTTTTATCCTAACAGCACAAAATATTTGCCTTTAGACAAAGGGATTAAAC9210.1538204592901879No Hit
CTCAAGGACAGTTTCTCTTCCAAAAGTAAGGCAAGAAATATGAAACCACA9190.1534864300626305No Hit
CCATTTACACAATGTGGTTACCCTGCATTAATGCCTTTGTATGCATGTAT9110.15215031315240082No Hit
GCTTTATTCTTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACCCCTT8950.14947807933194154No Hit
GTATACATGCATACAAAGGCATTAATGCAGGGTAACCACATTGTGTAAAT8790.14680584551148226No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTCACTGGG8640.14430062630480167No Hit
GTCCCTATCTTTAATCCTGAATGGCAAACCCCTTCTTTTCCTAAAATTCA8400.14029227557411272No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8330.1391231732776618No Hit
CTCATAAGGTGGGAAATTTCACTGGGCTTTATTCTTCTACTGTCCCTATC8070.13478079331941545No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCCGTCCG8050.13444676409185805No Hit
TCCTTGGACTCATAAGGTGGGAAATTTCACTGGGCTTTATTCTTCTACTG7970.13311064718162838No Hit
CCTTTAGACAAAGGGATTAAACCTTATTATCCTGATCATGTAGTTAATCA7940.13260960334029226No Hit
GGATAAAACCTAGCAGGCATAATTAATTTCAGTCTTCTTTTTTCATTTAC7850.13110647181628393No Hit
GTCTTCATGTAAATGAATTTTAGGAAAAGAAGGGGTTTGCCATTCAGGAT7840.13093945720250522No Hit
ATATAGAGCTGAGGCTGTATCTAGTAGATCCCGGACGGAAGGAAAGAAGT7740.12926931106471817No Hit
CTGTAAATGAAAAAAGAAGACTGAAATTAATTATGCCTGCTAGGTTTTAT7670.12810020876826722No Hit
GATCAGGATAATAAGGTTTAATCCCTTTGTCTAAAGGCAAATATTTTGTG7630.1274321503131524No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG7580.12659707724425887No Hit
CTCTCACTGTAAATGAAAAAAGAAGACTGAAATTAATTATGCCTGCTAGG7520.12559498956158663No Hit
CTACATGATCAGGATAATAAGGTTTAATCCCTTTGTCTAAAGGCAAATAT7410.12375782881002088No Hit
GGGTAAAGGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA7320.12225469728601253No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTCACTGGGCTTTATTC7300.12192066805845513No Hit
TTGTAGGCCCTCTCACTGTAAATGAAAAAAGAAGACTGAAATTAATTATG7150.11941544885177453No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6980.11657620041753654No Hit
GGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA6970.11640918580375782No Hit
ATACTAACATGGGCCTAAAGATCAGGCAATTATTGTGGTTTCATATTTCT6970.11640918580375782No Hit
CTTCCAAAGAGTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTA6750.1127348643006263No Hit
GAAATTAATTATGCCTGCTAGGTTTTATCCTAACAGCACAAAATATTTGC6690.11173277661795408No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6680.11156576200417535No Hit
CTGACATACTTTCCAATCAATAGGTCGATTGACAGGAAGTTTTCTAAAAC6540.10922755741127348No Hit
GGCCTAAAGATCAGGCAATTATTGTGGTTTCATATTTCTTGCCTTACTTT6420.10722338204592903No Hit
GCACAAAATATTTGCCTTTAGACAAAGGGATTAAACCTTATTATCCTGAT6310.10538622129436326No Hit
GTTAGGATAAAACCTAGCAGGCATAATTAATTTCAGTCTTCTTTTTTCAT6300.10521920668058456No Hit
ATTTTGTGCTGTTAGGATAAAACCTAGCAGGCATAATTAATTTCAGTCTT6270.10471816283924844No Hit
GAATTAATGACTCTAGCTACCTGGGTGGGTAATAATTTGGAAGATCCAGC6150.10271398747390398No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAGAGATGGGGTTATT6140.10254697286012526No Hit
TATCCTAACAGCACAAAATATTTGCCTTTAGACAAAGGGATTAAACCTTA6120.10221294363256786No Hit
ATATTTGCCTTTAGACAAAGGGATTAAACCTTATTATCCTGATCATGTAG6110.10204592901878916No Hit
CTATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATA6080.10154488517745303No Hit
GTATTAACATAATTGACTACTAGATCCCTGGATGCTGGATCTTCCAAATT6070.10137787056367431No Hit
CTTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACCCCTTCTTTTCCT6060.10121085594989561No Hit
TGTTAATACTAACATGGGCCTAAAGATCAGGCAATTATTGTGGTTTCATA6010.10037578288100209No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)